Different concentrations of adapalene induce differentiation and apoptosis of SH-SY5Y cells

LIU Na-Na; ZHANG Jun-Jiao; ZHANG Fan; WU Cong-Ying; JIANG Yu-Wu

doi:10.7499/j.issn.1008-8830.2310100

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Chinese Journal of Contemporary Pediatrics ›› 2024, Vol. 26 ›› Issue (3) : 282-288. DOI: 10.7499/j.issn.1008-8830.2310100

EXPERIMENTAL RESEARCH

Different concentrations of adapalene induce differentiation and apoptosis of SH-SY5Y cells

LIU Na-Na^1,2, ZHANG Jun-Jiao^1,2, ZHANG Fan^1,2, WU Cong-Ying³, JIANG Yu-Wu^1,2

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Abstract

Objective To investigate the effects of different concentrations of adapalene on the morphology and functions of neuroblastoma cell line SH-SY5Y, as well as its role in inducing cell differentiation and apoptosis. Methods SH-SY5Y cells were divided into control group, low concentration (0.1 μM and 1 μM) adapalene groups, and high concentration (10 μM) adapalene group. Time-lapse microscopy was used to observe the morphological changes of SH-SY5Y cells. Immunofluorescence staining was performed to detect the expression of neuronal specific marker βIII-tubulin and mature neuronal marker neurofilament heavy polypeptide (NFH). Multi-electrode array was used to record the electrophysiological features of SH-SY5Y cells. Cell apoptosis was evaluated using a cell apoptosis detection kit. Results Low concentrations of adapalene promoted the formation of neurite outgrowth in SH-SY5Y cells, with the neurites interconnected to form a network. Spontaneous discharge activity was observed in SH-SY5Y cells treated with low concentrations of adapalene. Compared to the control group, the expression of βIII-tubulin and NFH increased in the 1 μM adapalene group, while the level of cell apoptosis increased in the high concentration adapalene group (P<0.05). Conclusions Low concentrations of adapalene can induce differentiation of SH-SY5Y cells into mature functional neurons, while high concentrations of adapalene can induce apoptosis in SH-SY5Y cells.

Key words

Adapalene / Differentiation / Apoptosis / SH-SY5Y cell

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LIU Na-Na, ZHANG Jun-Jiao, ZHANG Fan, WU Cong-Ying, JIANG Yu-Wu. Different concentrations of adapalene induce differentiation and apoptosis of SH-SY5Y cells[J]. Chinese Journal of Contemporary Pediatrics. 2024, 26(3): 282-288 https://doi.org/10.7499/j.issn.1008-8830.2310100

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