胶体金法与化学发光法检测晨尿促性腺激素在评估儿童青春发育状态中的应用

doi:10.7499/j.issn.1008-8830.2409066

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Abstract Objective To explore the application of the colloidal gold method and chemiluminescence method in detecting gonadotropin (Gn) in morning urine for assessing pubertal development status in children. Methods A total of 132 children diagnosed with central precocious puberty (CPP), early and fast puberty (EFP), and premature thelarche (PT) at Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from November 2021 to December 2022 were included, along with 685 healthy children who underwent routine health examinations at the hospital's pediatric health care department during the same period. All 132 patients underwent a gonadotropin-releasing hormone (GnRH) stimulation test. Both patients and healthy children had their urinary Gn levels measured using the colloidal gold method and chemiluminescence method, including levels of luteinizing hormone (LH) and follicle-stimulating hormone (FSH). The correlation between serum Gn and urinary Gn detected by the two methods, as well as the correlation between Tanner stages of healthy children and urinary Gn, was analyzed. Results Urine Gn levels detected by both the colloidal gold method and chemiluminescence method showed a positive correlation with serum LH baseline values, LH peak values, baseline LH/FSH ratios, and peak LH/FSH ratios (P<0.05). In healthy children, urinary LH levels detected by the chemiluminescence method gradually increased from Tanner stage Ⅰ to Ⅳ (P<0.05), while urinary FSH levels were lower in Tanner stage I than in stages Ⅱ, Ⅲ, and IV (P<0.05). Urinary LH levels detected by the colloidal gold method were lower in Tanner stage I compared to stages Ⅱ, Ⅲ, and IV, with the highest levels observed in Tanner stage Ⅳ (P<0.05). Additionally, urinary FSH levels in Tanner stage Ⅲ were higher than in stages Ⅰ and Ⅱ (P<0.05). The area under the receiver operating characteristic curve for evaluating Tanner stages I and II in healthy children using urinary LH and FSH levels by the chemiluminescence method and urinary LH levels by the colloidal gold method were 0.730, 0.699, and 0.783, respectively. Conclusions The colloidal gold method and chemiluminescence method for detecting Gn in morning urine show good correlation with serum Gn levels. As a non-invasive and convenient detection method, the colloidal gold method can serve as a useful tool for screening the onset of pubertal development in children.

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	Articles by authors
	ZHAO Xue-Qi
	LU Wen-Li
	LI Wen-Ying
	WANG Jun-Qi
	DONG Zhi-Ya
	XIAO Yuan
	ZHANG Xiao-Fei
	JIANG Li
	MA Xiao-Yu

Key words： Central precocious puberty Colloidal gold method Chemiluminescence method Gonadotropin Pubertal development Child

Received: 10 September 2024

Corresponding Authors: Email：179788825@qq.com E-mail: 179788825@qq.com

Cite this article:

ZHAO Xue-Qi,LU Wen-Li,LI Wen-Ying et al. Application of colloidal gold method and chemiluminescence method for detecting gonadotropins in morning urine to assess pubertal development status in children[J]. CJCP, 2025, 27(2): 199-204.

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http://www.zgddek.com/EN/10.7499/j.issn.1008-8830.2409066 OR http://www.zgddek.com/EN/Y2025/V27/I2/199

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