Abstract OBJECTIVE: To study the effects of ganglioside 1 (GM1) and nerve growth factor (NGF) on neural stem cells (NSCs) proliferation in vitro. METHODS: NSCs were isolated and cultured in vitro. NSCs were cultured in the medium containing epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) or without the two agents. Different concentrations of GM1 and NGF were added into the two different medium. MTT and cell ball counting methods were used to ascertain the proliferation of NSCs. Immunohistochemical technology was used to observe the effect of GM1 and NGF on the proliferation of NSCs. RESULTS: High concentrations of GM1 (100 ng/L and 200 ng/L) promoted significantly the proliferation of NSCs in the medium containing EGF and bFGF (P<0.05). In the differentiation medium containing serum but no EGF and bFGF, NSCs proliferation increased with increasing concentration of GM1; the proportion of neurons and gliacytes increased with increasing concentration of NGF. CONCLUSIONS: High concentration of GM1 can promote NSCs proliferation and NGF can promote NSCs differentiation.［Chin J Contemp Pediatr, 2009, 11 (10):841-845］
WANG Qian,WANG Zhi-Ping,XU Qin et al. Effects of ganglioside 1 and nerve growth factor on the proliferation of neural stem cells in vitro[J]. 中国当代儿科杂志, 2009, 11(10): 841-845.
WANG Qian,WANG Zhi-Ping,XU Qin et al. Effects of ganglioside 1 and nerve growth factor on the proliferation of neural stem cells in vitro[J]. CJCP, 2009, 11(10): 841-845.
［1］Ishibashi S, Sakaguchi M, Hiroiwa T, Yamasaki M, Kanemura Y, Shizuko I, et al. Human neural stem/progenitor cells, expanded in long-term neurosphere culture, promote functional recovery after focal ischemia in Mongolian gerbils［J］. J Neurosci Res, 2004, 78 (2):215-223.
［2］Nishino H, Hida H, Takei N, Kumazaki M, Nakajima K, Baba H. Mesencephalic neural stem (progenitor) cells develop to dopaminergic neurons more strongly in dopamine-depleted striatum than in intactstriatum［J］. Exp Neurol, 2000, 164(1):209-214.
［3］Doering LC, Snyder EY. Cholinergic expression by a neural stem cell line grafted to the adult medial septum/diagonal band complex ［J］. J Neurosci Res, 2000, 61(6):597-604.
3.0.CO;2-L target="_blank">
［4］Schwartz M, Spirman N. Sprouting from chicken embryo dorsal root ganglia induced by nerve growth factor is specifically inhibited by affinity purified antianglioside antibodides［J］. Proc Natl Acad Sci USA, 1982, 79 (19):6080-6083.
［5］Cuello AC, Garofalo L, Kenigsberg RL, Maysinger D. Gangliosides potentiate in vivo and in vitro effects of nerve growth factor on central cholinergic neurons［J］. Proc Natl Acad Sci USA, 1989, 86 (6):2056-2060.
［6］Mckay R. Stem cells in the central nervous system［J］. Science, 1997, 276(5309):66-71.
［7］Freed CR, Breeze BE, Rosenberg NL, Schneck SA. Embryonic dopamine cell implants as treatment for the second phase of Parkinson’s disease. Replacing failed nerve terminals［J］. Adv Neurol, 1993, 60(8):721-728.
［8］Fiorio Pla A, Maric D, Brazer SC, Giacobini P, Liu X, Chang YH, et al. Canonical transient receptor potential 1 plays a role in basic fibroblast growth factor (bFGF)/FGF receptor-1-induced Ca2+ entry and embryonic rat neural stem cell proliferation［J］. J Neurosci, 2005, 25(10):2687-2701.
［9］Türeyen K, Vemuganti R, Bowen KK, Sailor KA, Dempsey RJ. EGF and FGF-2 infusion increases post-ischemic neural progenitor cell proliferation in the adult rat brain［J］.Neurosurgery, 2005, 57(6):1254-1263.
［10］Petruska JC, Mendell LM. The many functions of nerve growth factor: multiple actions on nociceptors［J］. Neurosci Lett, 2004, 361(1-3):168-171.
［14］Neto JC, Vasconcelos BC, Sobral AP, da Silva VA Jr, Noqueira RV. Clinical and histopathologic study of the use of gangliosides for nerve regeneration in rats after axonotmesis［J］. J Oral Maxillofac Surg, 2007, 65(5):870-874.
［16］Duchemin AM, Neff NH, Hadjiconstantinou M. GM1 increases the content and mRNA of NGF in the brain aged rats［J］. Neuroreport, 1997, 8(17):3823-3827.
［17］Baumgartner WA, Redmond JM, Zehr KJ, Brock MV, Tseng EE, Blue ME, et al. The role of the monosialoganglioside, GM1 as a neuroprotectant in an experimental model of cardiopulmonary bypass and hypothermic circulatory arrest［J］. Ann N Y Acad Sci, 1998, 845:382-390.
