CJCP
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2011 Vol.  13 No.  8
Published: 2011-08-15
CLINICAL RESEARCH
EXPERIMENTAL RESEARCH
CLINICAL EXPERIENCE
CASE REPORT
CLINICAL RESEARCH
611 ZHOU Xi-Hui, HUI Zhi-Yan, SHI Rui-Ming, SONG Hong-Xia, ZHANG Wei, LIU Li
Site-directed mutagenesis and protein expression of KCNQ2 gene associated with neonatal convulsions
OBJECTIVE: To study the protocol of construction of a KCNQ2-c.812G>T mutant and it′s eukaryotic expression vector, the c.812G>T (p.G271V) mutation which was detected in a Chinese pedigree of benign familial infantile convulsions, and to examine the expression of mutant protein in human embyonic kidney (HEK) 293 cells. METHODS: A KCNQ2 mutation c.812G>T was engineered on KCNQ2 cDNAs cloned into pcDNA3.0 by sequence overlap extension PCR and restriction enzymes. HEK293 cells were co-transfected with pRK5-GFP and KCNQ2 plasmid (the wild type or mutant) using lipofectamine and then subjected to confocal microscopy. The transfected cells were immunostained to visualize the intracellular expression of the mutant molecules. RESULTS: Direct sequence analysis revealed a G to T transition at position 812. The c.812G>T mutation was correctly combined to eukaryotic expressive vector pcDNA3.0 and expressed in HEK293 cells. Immunostaining of transfected cells showed the expression of both the wild type and mutant molecules on the plasma membrane, which suggested that the c.812G>T mutation at the pore forming region of KCNQ2 channel did not impair normal protein expression in HEK293 cells. CONCLUSIONS: Successful construction of mutant KCNQ2 eukaryotic expression vector and expression of KCNQ2 protein in HEK293 cells provide a basis for further study on the functional effects of convulsion-causing KCNQ2 mutations and for understanding the molecular pathogenesis of epilepsy.
2011 Vol. 13 (8): 611-616 [Abstract] ( 5379 ) [HTML 1KB] [PDF 1614KB] ( 1474 )
617 LI Xiao, CHEN Yi-Ji, LI Lu-Quan
Relationship between viral burden in urine and hearing loss in neonates with cytomegalovirus infection
OBJECTIVE: To determine the relationship between viral burden in urine and hearing loss in neonates with cytomegalovirus (CMV) infection. METHODS: Twenty-two neonates with CMV infection between April 2006 and January 2010 were enrolled. Their viral burden in urine and hearing loss information were studied. The receiver operating characteristic curve (ROC) was constructed and the cutoff was determined based on their medical information. The hearing levels were evaluated by brain stem auditory evoked potential (BAEP) during the age of 3 to 6 months in 20 patients. RESULTS: The viral burden in urine in neonates with abnormal BAEP was higher than that in neonates with normal BAEP (5.06±1.50 vs 3.73±0.86, P<0.05). Hearing loss was predicted with a sensitivity of 0.545 and a specificity of 1.0 by using ROC at the cutoff point of 5.1 which were defined after logarithmic conversion at 1.27×105 copies/mL of CMV burden in urine. The incidence of hearing loss during the age of 3 to 6 months was strikingly higher in high viral burden group than that in low viral load group (P<0.05). CONCLUSIONS: The viral burden in urine can predict the possibility of hearing loss in neonates with CMV infection. Hearing loss is likely to be developed when viral burden in urine ≥1.27×105 copies/mL in neonates with CMV infection.
2011 Vol. 13 (8): 617-620 [Abstract] ( 4776 ) [HTML 1KB] [PDF 946KB] ( 1321 )
621 MAO Jian, LI Juan, CHEN Dan, ZHANG Jing, DU Ya-Nan, WANG Ying-Jie, LI Xin, WANG Rui, CHEN Li-Ying, WANG Xiao-Ming
Value of MRI in the diagnosis of cerebral abscess caused by Candida albicans in premature infants
OBJECTIVE: To explore the value of serial magnetic resonance imaging (MRI) in the diagnosis of cerebral abscess caused by Candida albicans in premature infants. METHODS: The clinical data of 8 premature infants with central nervous system invasive fungal infection (IFI) were retrospectively studied. The infants underwent serial cerebral MRI scans (T1WI, T2WI and DWI). RESULTS: Candida albicans was found as pathogen in all of the 8 infants. Seven infants presented with cerebral abscess and 4 infants had concurrent meningitis. Widespread involvements were found on MRI, particular in white matter area of subcortex, centrum semiovale and periventricle. The MR imaging findings in 4 infants within 11 days after IFI showed diffusive and multiple miliary nodes and hyperintense signals on DWI, but obvious changes were not found on T1WI and T2WI. The most striking hyperintense signals on T1WI and hypointense signals on T2WI appeared between 2 and 4 weeks after IFI, and some nodes of rim-like hyperintensity and marked contrast enhancement were also noted on T1WI. Smaller and smaller changes of the miliary foci were seen on T1WI and T2WI 4 weeks later. Delayed myelination and thinner corpus callous were shown in 2 patients at three months. CONCLUSIONS: MRI-DWI and serial MRIs are helpful in the early diagnosis of candida cerebral abscess and the evaluation of treatment outcome in premature infants.
2011 Vol. 13 (8): 621-626 [Abstract] ( 5167 ) [HTML 1KB] [PDF 1165KB] ( 1671 )
627 TANG Zhen, ZHOU Ying, LI Ming-Xia
Clinical features of feeding intolerance in preterm infants
OBJECTIVE: To study the clinical features of feeding intolerance in preterm infants in order to provide clinical evidence for preventing feeding intolerance. METHODS: A total of 716 preterm infants hospitalized in the First Affiliated Hospital of Xinjiang Medical University between January 2007 and December 2009 were enrolled. The clinical data of the infants were retrospectively studied. RESULTS: Feeding intolerance occurred in 197 (27.5%) out of 716 infants, with an incidence of 76.4% in the very low birth weight infants. Simple gastric retention (47.2%) was found as the most common clinical manifestation. Feeding intolerance usually occurred within 3 days after feeding. Compared with the preterm infants with feeding success, the preterm infants with feeding intolerance showed lower gestational age and birth weight, more delayed feeding and higher rate of asphyxia and respiratory diseases (P<0.05). There were no significant differences in the gender, maternal age, ethnic group, delivery way and the incidence of fetal distress between the two groups. With increasing gestational age, increasing birth weight, early feeding, the incidence of feeding intolerance in preterm infants was reduced. CONCLUSIONS: The low gestational age, low birth weight, delayed feeding and complications may be associated with the development of feeding intolerance in preterm infants.
2011 Vol. 13 (8): 627-630 [Abstract] ( 6984 ) [HTML 1KB] [PDF 972KB] ( 1920 )
631 HAN Tong-Xin, LI Cai-Feng, WANG Jiang, KUANG Wei-Ying, ZHOU Yi-Fang
Efficacy of thalidomide for treatment of juvenile idiopathic arthritis
OBJECTIVE: To evaluate the efficacy of thalidomide in the treatment of juvenile idiopathic arthritis (JIA). METHODS: Twelve children with JIA who did not respond to conventional treatment were administered with thalidomide (2 mg/kg daily). The symptoms, signs, and laboratory test results were compared before and after treatment. The thalidomide-related side effects were observed. RESULTS: The average dosage of prednisone was reduced from 1.92±0.16 mg/kg?d to 0.49±0.42 mg/kg?d in the 12 patients 6 months after thalidomide treatment (P<0.01). Four patients did not need prednisone treatment any more. White blood cell count, erythrocyte sedimentation rate (ESR), C reactive protein (CRP) and serum ferritin (SF) significantly decreased after treatment in all of 12 patients (P<0.01). Hemoglobin level increased to normal in 8 patients after treatment (P<0.01). The number of affected joints decreased from 5 before treatment to zero to 2 after treatment in patients with polyarticular JIA (P<0.01). Signs of hip involvement and Schober′s sign turned negative in enthesitis-related cases. No thalidomide-related side effects were observed. CONCLUSIONS: Thalidomide is effective in the treatment of JIA in children who do not respond to conventional treatment.
2011 Vol. 13 (8): 631-634 [Abstract] ( 5467 ) [HTML 1KB] [PDF 910KB] ( 1387 )
635 JIA Lei, ZHAO Cheng-Song, ZHANG Li, LI Shuang, ZHANG Dai-Tao, LIU Bai-Wei, WANG Quan-Yi, LI Xin-Yu
Comparisons of epidemiological and clinical characteristics in children with hand-foot-mouth disease caused by Enterovirus 71 and Coxackievirus A16
OBJECTIVE: To compare the differences of epidemiological and clinical characteristics in children with hand-foot-mouth disease (HFMD) caused by Coxsackievirus A16 (CA16) and Enterovirus 71 (EV71). METHODS: The samples of vesicle fluid and throat swabs of 108 children with HFMD were collected and detected for enterovirus by RT-PCR. The clinical data of children with EV71 and CA16 infection were retrospectively reviewed and compared. RESULTS: The total positive rate of enterovirus was 97.2% (105/108). Of the 105 cases, 56 cases were positive for EV71 (51.9%), 39 cases were positive for CA16 (36.1%), 2 cases were positive for other enterovirus (1.9%), and 8 cases were co-infected by EV71 and CA16 (7.4%). There were no significant differences in age and sex between EV71 and CV16 infected cases. The univariate analysis showed that the incidences of herpes of mouth, erythra of knees, and nose running in children infected by CA16 were higher than in those infected by EV71. The multivariate logistic regression analysis showed that the HFMD children who had erythra of knees had higher probability of CA16 infection. CONCLUSIONS: EV71 should be considered as the pathogen in children with HFMD who have no herpes of mouth, erythra of knees, and nose running.
2011 Vol. 13 (8): 635-637 [Abstract] ( 6609 ) [HTML 1KB] [PDF 903KB] ( 1849 )
638 CHEN Jing, MA Bin, LIN Li-Xing, XUE Yi-Ming
Treatment of Kawasaki disease by different doses of immunoglobulin: a Mata analysis of therapeutic effects
OBJECTIVE: To assess the therapeutic effects and safety of different doses of immunoglobulin in the treatment of Kawasaki disease. METHODS: The papers related to the treatment of Kawasaki disease were electronically searched in the databases of PubMed, EMBASE, Cochrane Library, CNKI, VIP and Wanfang. Randomized clinical trials (RCT) on the treatment of Kawasaki disease with different doses of immunoglobulin were included and assessed for quality. A Mata analysis was performed by RevMan 5.0. RESULTS: Twenty-eight RCTs involved 2596 cases were included. The results of Meta analysis showed that there were no significant differences in the incidences of coronary artery injuries at various phases, adverse effects and fever disappearance time between the immunoglobulin treatment groups at the doses of 1 g/(kg?d) for 1-2 days and 2 g/(kg?d) for single use. The fever disappearance time in the immunoglobulin treatment group at the dose of 1 g/(kg?d) for 1-2 days was significantly shorter than that in the immunoglobulin treatment group at the dose of 400 mg/(kg?d) for 4-5 days, but there were no significant differences in the incidences of coronary artery injuries at the acute phase and 6 months after treatment and adverse effects between the two groups. The incidence of coronary artery injuries at the acute phase and 6 months was lower and the fever disappearance time was shorter in the immunoglobulin treatment group at the dose of 2 g/(kg?d) for single use than those in the immunoglobulin treatment group at the dose of 400 mg/(kg?d) for 4-5 days, but there were no significant differences in the incidences of coronary artery injuries at the subacute phase and 12 months after treatment and adverse effects between the two groups. CONCLUSIONS: There are similar therapeutic effects for Kawasaki disease between the immunoglobulin treatment groups at the doses of 1 g/(kg?d) for 1-2 days and 2 g/(kg?d) for single use. The fever disappearance time in the two groups is shorter than that in the treatment group at the dose of 400 mg /(kg?d) for 4-5 days.
2011 Vol. 13 (8): 638-643 [Abstract] ( 6103 ) [HTML 1KB] [PDF 944KB] ( 1804 )
644 ZHANG Wei-Li, ZHAO Juan, LI Wei
Influencing factors of mother-infant vertical transmission of hepatitis B virus
OBJECTIVE: To study the factors influencing mother-infant vertical transmission of hepatitis B virus (HBV). METHODS: A total of 635 pregnant women with chronic hepatitis B or chronic asymptomatic HBV carriers were enrolled. The rate of HBV infection was compared between the infants born from the pregnant women of different HBV-DNA load, different ways of delivery and different liver functions at birth and 3 months after birth. The newborn infants were routinely injected with hepatitis B immunoglobulin (200 IU) and hepatitis B vaccine (10 μg) within 12 hrs of birth. The newborns presenting HBV infection within 24 hrs of birth by serum test were re-injected with hepatitis B immunoglobulin (200 IU) 14 days after birth. RESULTS: The rate of HBV infection in infants with maternal HBV-DNA load >105 copies/mL was higher than in those with maternal HBV-DNA load ≤105copies/mL at birth (14.4% vs 4.1%; P<0.01) and 3 months after birth (4.7% vs 0; P<0.01).The rate of HBV infection at 3 months was lower than at birth in both groups. The rate of HBV infection in infants born by natural labor was higher than in those born by caesarean birth at birth (P<0.05), however, by 3 months after birth, the rate of HBV infection between the two groups was similar. The rate of HBV infection was higher in infants born to chronic asymptomatic HBV carrier mothers than that in infants born to chronic hepatitis B mothers at birth (P<0.01), but there were no significant differences in the two groups 3 months later. CONCLUSIONS: The maternal HBV-DNA load is correlated with the rate of HBV infection of infants. It might thus be an effective way to reduce the rate of HBV infection in infants by decreasing maternal HBV-DNA load. With the administration of hepatitis B immunoglobulin and hepatitis B vaccine, the delivery way and the liver function of pregnant women may not to be factors influencing mother-infant HBV vertical transmission.
2011 Vol. 13 (8): 644-646 [Abstract] ( 4938 ) [HTML 1KB] [PDF 908KB] ( 1677 )
647 PAN Xiao-Li, ZHANG Nan-Nan, YE Hong-Lian, ZHAO Yue-Fei, GAO Hong
Studies of electromyography and genetics in children with Charcot-Marie-Tooth disease type 1
OBJECTIVE: To study the electromyographic and genetic characteristics in children with Charcot-Marie-Tooth disease type 1 (CMT1). METHODS: Routine electromyography and nerve conduction were performed in 24 children with CMT1. Polymerase chain reaction (PCR) combined with restriction enzyme digestion was used to detect gene duplication on chromosome 17p11.2-12. Ten healthy children served as the control group. RESULTS: The peripheral nerve conduction velocity slowed or disappeared in all of the 24 patients (100%). The lesions of the sensory nerves were more severe than the motor nerves, and the lesions of the lower limbs were more severe than the upper limbs. Of 72 muscles detected, 40 (56%) showed neurogenic lesions. The older the patients, the more severe the muscle lesions. Specific junction fragments (1760 bp) were identified in 13 (54%) out of 24 patients, but were not identified in the healthy controls. CONCLUSIONS: The electromyographic changes are characterized by peripheral nerve conduction velocities slowing and neurogenic lesions of muscles in children with CMT1. The PCR combined with restriction enzyme digestion may be a simple and accurate method for gene diagnosis of CMT1.
2011 Vol. 13 (8): 647-650 [Abstract] ( 4550 ) [HTML 1KB] [PDF 934KB] ( 1182 )
651 WANG Fang, YANG Yao, YANG Xiao, WANG Chun-Zhi, HE Xi-Yu
Chromosome analysis in 92 children with congenital mental retardation
OBJECTIVE: To explore the chromosome karyotypes in children with mental retardation. METHODS: The peripheral blood lymphocytes from 92 chidren with congenital mental retardation were cultured and analysed by the G-band technique. RESULTS: Of the 92 cases, 43 cases (47%) showed chromosome abnormalities. Autosomal abnormalities were found in 35 cases (38%) and sex chromosome abnormalities were found in 8 cases (9%). A novel abnormal karyotype 45,XX,psu dic（11;9）（p15;p24）was found in a child. CONCLUSIONS: Chromosome abnormalities may be important cytogenetic factors for congenital mental retardation. Cytogenetic chromosome karyotypic analysis appears to be an important method for genetic screening of congenital mental retardation.
2011 Vol. 13 (8): 651-653 [Abstract] ( 4483 ) [HTML 1KB] [PDF 928KB] ( 1611 )
654 MA Qi-Ling, WANG Bo, FU Guo-Hua, CHEN Guang-Fu, CHEN Zhen-Yan
Heart rate variability in children with beta-thalassemia major
OBJECTIVE: To study the diagnostic value of heart rate variability (HRV) in heart dysfunction in children with beta-thalassemia major (β-TM)by examining the changes of HRV in β-TM children. METHODS: A 24 hours Holter monitoring electrocardiogram (Holter) was performed in 21 children with β-TM and 15 healthy children (control group). The time domain and frequency domain indexes of HRV in the two groups were compared. The correlation between serum ferritin levels and HRV was evaluated. RESULTS: The time domain indexes SDNN, rMSSD and PNN50 and the frequency domain indexes very low frequency (VLF), low frequency (LF) and high frequency (HF) in the β-TM group were significantly lower than in the control group (P<0.05). There was no correlation between serum ferritin level and HRV in children with β-TM. CONCLUSIONS: The autonomic nerve dysfunction exists in children with β-TM. HRV analysis is useful in the prediction of early cardiac dysfunction in children with β-TM.
2011 Vol. 13 (8): 654-656 [Abstract] ( 4993 ) [HTML 1KB] [PDF 901KB] ( 1481 )
657 ZHAO Min, FENG Chen, WANG Jian-Wen, LIU Ying, TANG Suo-Qin
Childhood rhabdomyosarcoma: a retrospective review of 23 cases
OBJECTIVE: To study the clinical characteristics, treatment and outcome of childhood rhabdomyosarcoma. METHODS: The clinical data of 23 children with rhabdomyosarcoma from January, 1998 to October, 2008 were retrospectively reviewed. RESULTS: Of the 23 cases, 15 were male and 8 were female, with a mean age of 5 years old (7 months to 12 years old). Based on the American IRS staging system, 2 cases were in stage I, 4 cases in stage II, 8 cases in stage III, and 9 were in stage IV. The primary sites were found in head and neck (14 cases), extremities (4 cases), bladder (2 cases), kidney (1 case), postperitoneum (1 case) and bile duct (1 case). All of the children were confirmed with rhabdomyosarcoma by biopsy and immunohistochemistry. The clinical manifestations were related to the tumor tissues-induced space occupying, compression and erosion and were aspecific. The patients in different IRS stages were given different treatment regimens. The chemotherapy regimens VDCA, VAC or VadrC were used before 2002. After 2002, the Children′s Oncology Group (COG) protocol was employed. The two-year survival rate was 63% in 19 patients who received a combination of surgery, chemotherapy and radiotherapy, but none of 4 patients who received a surgery alone or a combination of surgery and chemotherapy or radiotherapy survived more than two years. CONCLUSIONS: The clinical manifestations of childhood rhabdomyosarcoma are not specific. A combination therapy including surgery, chemotherapy and radiation is effective to the improvement of the survival rate in children with rhabdomyosarcoma.
2011 Vol. 13 (8): 657-660 [Abstract] ( 5546 ) [HTML 1KB] [PDF 910KB] ( 2032 )
EXPERIMENTAL RESEARCH
661 JING Ke, SUN Mei
Relationship between the regulation of intestinal NF-κB and TNF-α by glutamine and the protective effects of glutamine against intestinal injury
OBJECTIVE: To study whether the protective effects of glutamine against intestinal injury are associated with the regulation of intestinal secretion of NF-κB and TNF-α by glutamine. METHODS: Twenty-four 10-day-old Wistar rat pups were randomly intraperitoneally injected with normal sodium of 1 mL/kg (control group; n=8), LPS of 5 mg/kg (endotoxemia group; n=8) or LPS of 5 mg/kg plus glutamine of 10 mL/kg (glutamine group; n=8). The rats were sacrificed 3 hrs after injection. A segment of distal ileum was dissected. The pathologic changes of the small intestine were observed under an optical microscope (hematoxylin-eosin staining). The expression of intestinal NF-κB mRNA and protein were detected by RT-PCR and Western blot respectively. The TNF-α level in intestinal tissues was measured using ELISA. RESULTS: The structure of the small intestine in the control group remained normal. The inflammation cells infiltration and the edema of interstitial substance and epithelials were observed in the endotoxemia and glutamine groups. The pathologic changes in the glutamine group were significantly alleviated when compared with the endotoxemia group. The expression of NF-κB mRNA and protein in the glutamine group was significantly lower than that in the endotoxemia group (P<0.01). The secretion of TNF-α in the glutamine group was significantly lower than that in the endotoxemia group (P<0.01). CONCLUSIONS: The protective effects of glutamine against intestinal injury may be associated with the down-regulation of intestinal NF-κB mRNA and protein expression and the reduction of the secretion of TNF-α.
2011 Vol. 13 (8): 661-664 [Abstract] ( 4558 ) [HTML 1KB] [PDF 1275KB] ( 1327 )
665 XU Jun, HUANG Rong, YANG Yu-Jia, JIN Shi-Jie, ZHANG Jin-Feng
Effects of baicalin on apoptosis in rats with autoimmune encephalomyelitis
OBJECTIVE: To study the therapeutic efficacy of baicalin and its effect on apoptosis of inflammatory cells in spinal cords in Wistar rats with autoimmune encephalomyelitis (EAE). METHODS: Forty-four rats were randomly divided into four groups: normal control group (control, n=10), EAE group (n=12), and two intervention groups with dexamethasone (DXM) or baicalin. Seven days after immunization, the two intervention groups were injected intraperitoneally with DXM (1 mg/kg) and baicalin (200 mg/kg) for 1 week, respectively. The spinal cords were removed 14 days after immunization, and stained with hematoxylin and eosin. MBP expression in spinal cords was detected by immunohistochemistry. The apoptosis of inflammatory cells in spinal cords was detected by TUNEL. RESULTS: The weight gain rate in the untreated EAE and the DXM or baicalin intervention groups were significantly lower than that in the control group (P<0.05). The weight gain rate in the baicalin intervention group was significantly higher than that in the untreated EAE and the DXM intervention groups (P<0.05). The scores of neurological function in the two intervention groups were significantly higher than that in the untreated EAE group (P<0.05). DXM or baicalin treatment significantly increased the MBP expression compared with the untreated EAE group (P<0.05). The apoptosis of inflammatory cells increased more in the DXM and the baicalin intervention groups compared with the untreated EAE groups (P<0.05). CONCLUSIONS: Baicalin has protective effects against EAE in rats. It can promote the apoptosis of inflammatory cells in spinal cords.
2011 Vol. 13 (8): 665-668 [Abstract] ( 4941 ) [HTML 1KB] [PDF 988KB] ( 1056 )
669 YANG Min, CHEN Chun-Yuan, CAI Zi-Li, CHEN Bai-Lin, CHENG Liang, LI Hui
Expression of matrix metalloproteinase-9 in myocardium of mice with viral myocarditis
OBJECTIVE: To investigate the dynamic changes of expression of matrix metalloproteinases-9 in myocardium of mice with viral myocarditis (VMC) and its significance in the pathogenesis of viral myocarditis. METHODS: VMC model was prepared by an injection of CVB3 in BALB/C mice. The mice receiving an injection of culture solution without virus were used as the control group. Cardiac tissues were obtained 7, 14, 21 and 28 days after injection and made into paraffin sections. Myocardial histopathologic changes were observed by hematoxylin-eosin staining and Masson staining. The expression of MMP-9, type I collagen and type III collagen in cardiac tissues were quantified by SABC immunohistochemical method. RESULTS: The expression of MMP-9 in the VMC model group was observed on the 7th day, reached a peak on the 14th day, and was significantly higher than that in the control group at all time points (P<0.05). Compared with the control group, the expression of type I collagen in the VMC model group was up-regulated on the 21st day and reached a peak on the 28th day (P<0.05). The expression of type III collagen in the VMC model group was significantly higher than that in the control group on the 28th day (P<0.05). The expression of MMP-9 was positively correlated with myocardial histopathologic scores (r=0.832, P<0.05) and negatively correlated with type I collagen expression (r=-0.791, P<0.05). CONCLUSIONS: MMP-9 is over-expressed at the early stage in VMC mice, and participates in the pathological process of VMC through mediating the degradation metabolism of type I collagen. It may be an important factor that leads to myocardial collagen remodeling and myocardial fibrosis.
2011 Vol. 13 (8): 669-673 [Abstract] ( 4764 ) [HTML 1KB] [PDF 1530KB] ( 1144 )
674 JIA Guo-Cun, TANG You-Cai, LI Feng-Yi, LIAO Qing-Kui
Deferoxamine induces apoptosis of K562 cells
OBJECTIVE: To study the molecular mechanism of apoptosis of leukemic cells (K562 cells) induced by iron chelating agent deferoxamine (DFO). METHODS: The exponentially growing K562 cells were used (1×106/mL) in this study. The K562 cells were treated with different concentrations of DFO (10, 50 and 100 mmol/L), DFO+FeCl3 (10 μmol/L each) or normal saline (blank control). The cellular labile iron pool was measured with a fluorimetric assay using the metalsensitive probe calcein-AM. The viable count and cell viability were determined by typanblue assay. Cell apoptosis was determined by morphological study and flow cytometry assay. Caspase-3 activity in K562 cells was detected by colorimetry. RESULTS: After DFO treatment, the cellular labile iron pool and the viability of K562 cells were reduced and the cell apoptosis increased in a time- and dose-dependent manner compared with the blank control group. The apoptosis rate of K562 cells in the DFO+FeCl3 treatment group was not significantly different from that in the blank control group. The caspase-3 activity in K562 cells increased significantly 24 hrs after 50 and 100 μmmol DFO treatment when compared with the blank control group (P<0.01). There was a negative correlation between cellular labile iron pool and caspase-3 activity of K562 cells (r=-0.894, P<0.05). CONCLUSIONS: DFO induces apoptosis of leukemic cells possibly through decreasing cellular labile iron pool and increasing caspase-3 activity of the cells.
2011 Vol. 13 (8): 674-676 [Abstract] ( 5365 ) [HTML 1KB] [PDF 978KB] ( 1587 )
677 XIAO Zhao-Hua, ZHOU Jian-Hua, WU Heng-Sheng
Effect of myriocin on the expression of cyclinD1 in high glucose-induced hypertrophy mesangial cells
OBJECTIVE: Myriocin (ISP-1) is a new type of immune inhibitor extracted from cordyceps sinensis. This study was to observe the effects of ISP-1 on the expression of cell cycle regulatory protein D1 (cyclinD1) in high glucose-induced hypertrophy rat glomerular mesangial cells (GMCs). METHODS: Rat GMCs were cultured in vitro and divided into three groups: high glucose (450 mg/dL D-glucose), normal glucose (100 mg/dL D-glucose, control) and ISP-1 (450 mg/dL D-glucose plus 100 μg/mL ISP-1). The protein expression of cyclinD1 was detected by flow cytometry. RESULTS: The expression of cyclinD1 in GMCs in the high glucose group increased significantly in a time-dependent manner compared with that in the control group. ISP-1 treatment significantly inhibited the up-regulated expression of cyclinD1 induced by high concentration glucose, and the expression of cyclinD1 was restored to the level of the control group 48 and 72 hrs after ISP-1 treatment. CONCLUSIONS: High concentration of glucose can up-regulate the expression of cyclinD1 in GMCs. ISP-1 may inhibit the up-regulated expression of cyclinD1, which might contribute to the protective effect of ISP-1 against GMC hypertrophy induced by high glucose.
2011 Vol. 13 (8): 677-679 [Abstract] ( 5144 ) [HTML 1KB] [PDF 970KB] ( 1131 )
680 XU Hui-Zhuo, LIU Shuang-Zhen, XIONG Si-Qi, XIA Xiao-Bo
HIF-1α siRNA reduces retinal neovascularization in a mouse model of retinopathy of prematurity Hot!
OBJECTIVE: To study the inhibition effect of HIF-1α specific siRNA expression vector pSUPERH1-siHIF-1α on retinal neovascularization in a mouse model of retinopathy of prematurity (ROP). METHODS: The mouse model of ROP was prepared by the method Smith described. Forty-eight ROP mice were randomly divided into two groups: an experimental group that was intravitreously injected with pSUPERH1-siHIF-1α and a control group that was injected with pSUPER retro vector. The levels of HIF-1α and vascular endothelia growth factor (VEGF) in the retina were examined by Western blot. The retinal neovascularization was evaluated by angiography using FITC Dextran and quantitated histologically. RESULTS: The levels of HIF-1α and VEGF in the retina in the experimental group were reduced 90% and 65% respectively compared with those in the control group. Meanwhile, the number of retinal neovascular endothelial nucleus outbreaking the inner limit membrane in the experimental group was significantly reduced compared with that in the control group. CONCLUSIONS: The development of retinal neovascularization of ROP can be markedly inhibited by RNA interference targeting HIF-1α.
2011 Vol. 13 (8): 680-683 [Abstract] ( 5521 ) [HTML 1KB] [PDF 1095KB] ( 1478 )
CLINICAL EXPERIENCE
684 LI Dan, YANG Xiang-Hong, CHANG Xiao-Ying
Clinical and pathological analysis of mesenchymal hamartoma of liver
No abstract available
2011 Vol. 13 (8): 684-685 [Abstract] ( 3603 ) [HTML 1KB] [PDF 1044KB] ( 1303 )
686 QIU Hui-Xian, ZHANG Yuan-Hai, CHEN Qi, WU Rong-Zhou, XIANG Ru-Lian
Clinical analysis of congenital heart disease complicated by other malformations
No abstract available
2011 Vol. 13 (8): 686-687 [Abstract] ( 4072 ) [HTML 1KB] [PDF 891KB] ( 988 )
CASE REPORT
688 LU Shan, CHEN Xiao-Feng, WANG Xue-Mei
A case report of incessant atrial tachycardia complicated by other malformations
No abstract available
2011 Vol. 13 (8): 688-689 [Abstract] ( 3458 ) [HTML 1KB] [PDF 942KB] ( 978 )
690 WANG Guo-Hua, YAN Chao-Ying, BAO Wan-Guo
Hand-foot-mouth disease complicated by brainstem encephalitis and cerebral edema in a neonate
No abstract available
2011 Vol. 13 (8): 690-691 [Abstract] ( 3851 ) [HTML 1KB] [PDF 880KB] ( 1390 )
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