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2011 Vol.  13 No.  10
Published: 2011-10-15
CLINICAL RESEARCH
EXPERIMENTAL RESEARCH
CLINICAL EXPERIENCE
CASE REPORT
771 HUANG Zhi-Heng, SUN Yi, CHEN Chao
Progress in encephalopathy of prematurity
The preterm birth has been increasing for the last decade. With the development of neonatal intensive care techniques, the survival rate of preterm infants is increased markedly. However, the brain of preterm infants is so vulnerable to injury that preterm brain injury has become an enormous public health problem. Hypoxia-ischemia and infection/inflammation are two main perinatal risk factors causing premyelinating oligodendrocyte and cortical neuron injury. Encephalopathy of prematurity is characterized by diffuse white matter injury and neuronal/axonal disruption, leading to neurological disabilities such as cognitive impairment and cerebral palsy. The advancement in imaging techniques, especially magnetic resonance imaging, provides more information for preterm brain injury and brain development, which contributes to the diagnosis and follow-up of the preterm infants. This article reviews the progress in encephalopathy of prematurity in order to open a new window to prophylaxis and management of this disease.
2011 Vol. 13 (10): 771-775 [Abstract] ( 7484 ) [HTML 1KB] [PDF 1007KB] ( 2789 )
832 LENG Qian, CUI Rui-Qin, LU Biao
Effects of multi-glycosides of tripterygium wilfordii on histological structures and c-kit expression in testes of pubertal rats Hot!
OBJECTIVE: To study the short- and long-term effects of multi-glycosides of tripterygium wilfordii (GTWon) the histological structures of testes in pubertal rats and possible mechanisms. METHODS: Forty-eight 5-week-old male Sprague-Dawley rats were randomly intragastrically administered with low-does GTW（6 g/kg daily）and high-does GTW (12 mg/kg daily) or 1% sodium carboxymethylcellulose (6 mL/kg, control group) for four weeks. The testes were sampled for detecting histological structures and c-kit expression by immunohistochemistry 24 hrs and four weeks after drug discontinuance. RESULTS: The number of spermatogenic cells and the expression of c-kit in testes were reduced in the two GTW treatment groups 24 hrs and 4 weeks after drug discontinuance compared with those in the control group(P<0.05). Four weeks after drug discontinuance atrophy and interstitial edema of seminiferous epitheliumin in testes were observed, and the testis weight and the expression of c-kit in testes were reduced in the high-does GTW group compared with those in the control group (P<0.01). There were no significant differences in the parameters observed between the low-dose GTW and the control group 4 weeks after drug discontinuance. CONCLUSIONS: GTW has adverse effects on testes in a dose-dependent manner in puberty rats. Low-dose GTW may cause reversible short-term injuries to testis tissues. The damage of the interstitial tissue of testes induced by high-dose GTW may be one of the causes of long-term injuries of testes.
2011 Vol. 13 (10): 832-836 [Abstract] ( 4940 ) [HTML 1KB] [PDF 1441KB] ( 1723 )
CLINICAL RESEARCH
776 ZHONG Ying, LI Juan, WEI Ke-Lun
Measurement of umbilical activin A level in preterm infants
OBJECTIVE: To evaluate the clinical significance of umbilical activin A in preterm infants. METHODS: Forty-one preterm infants (gestation 28 to 36 weeks) were enrolled. Fetal membranes, umbilical cords and blood samples from umbilical vein were obtained. Umbilical activin A level was measured using ELISA. The histological examinations of fetal membranes and umbilical cords were performed. RESULTS: The umbilical level of activin A averaged 2069 pg/mL in the 41 preterm infants. The umbilical activin A level in the 5 infants with intrauterine infection was higher than in those without intrauterine infection (2510 pg/mL vs 1975 pg/mL; P<0.01). Umbilical activin A level at cutoff of 2490 pg/mL showed a sensitivity of 80.0% and a specificity of 90.6% as a marker of intrauterine infection. There were no significant differences in the umbilical activin A level between the infants with and without respiratory distress syndrome. Umbilical activin A level was positively correlated with the duration of postnatal oxygen therapy (r=0.326, P<0.05). CONCLUSIONS: Umbilical activin A may serve a marker of intrauterine infection in preterm infants. The umbilical activin A level is correlated with the duration of postnatal oxygen therapy.
2011 Vol. 13 (10): 776-779 [Abstract] ( 4732 ) [HTML 1KB] [PDF 999KB] ( 1743 )
780 XU Fa-Lin, ZHUANG Fang-Li, BAI Qiong-Dan, DUAN Jia-Jia
Perinatal conditions of preterm infants with different severities of respiratory distress syndrome
OBJECTIVE: To understand the risk factors for respiratory distress syndrome (RDS) by comparing the perinatal conditions of preterm infants with different severities of RDS. METHODS: A total of 667 preterm infants with RDS were classified into 4 groups according to the chest X-ray severity: grade I (217 cases), grade II (225 cases), grade III (126 cases) and grade IV (99 cases). The perinatal conditions of the preterm infants were reviewed retrospectively. RESULTS: There were no significant differences in the gender, the percentage of twins, the percentage of the younger one in twins, maternal age, the percentage of using antenatal corticosteroids, the percentage of premature rupture of membranes, the percentage of placental abruption, the delivery mode and the fertilization mode in preterm infants with different severities of RDS. With the increasing severity of RDS, the birth weight and the gestational age decreased, and the percentage of the infants with Apgar score ≤7 or maternal pregnancy-induced hypertension increased (P<0.05). CONCLUSIONS: The severity of RDS is related to gestational age, birth weight and perinatal asphyxia in preterm infants.
2011 Vol. 13 (10): 780-782 [Abstract] ( 5534 ) [HTML 1KB] [PDF 907KB] ( 1792 )
783 LIN Xin-Zhu, ZHENG Zhi, LIN Ya-Yin, LAI Ji-Dong, LI Ya-Dan
Nasal synchronized intermittent positive pressure ventilation for the treatment of apnea in preterm infants
OBJECTIVE: To compare the efficacy of nasal synchronized intermittent positive pressure ventilation (NSIPPV) and nasal continuous positive airway pressure ventilation (NCPAP) for the treatment of apnea in preterm infants. METHODS: Eighty preterm infants with apnea from August 2010 to January 2011 were randomly administered with NSIPPV and NCPAP (n=40 each).The blood gas results before and 2 hrs after ventilation, time of using ventilator, therapeutic efficacy and complications were compared between the two groups. RESULTS: There were no significant differences in the blood gas results between the two groups before ventilation. The blood gas results (pH, PO2, PCO2) in the NSIPPV group were better than those in the NCPAP group 2 hrs after ventilation. The time of using ventilator in the NSIPPV group was shorter than that in the NCPAP group (50±9 h vs 91±11 h; P0.05). The proportion of ventilator weaning within 3 days in the NSIPPV group (23/40) was higher than that in the NCPAP group (14/40) (P<0.05). The incidence of complications in the NSIPPV group was not different from that in the NCPAP group (22% vs 25%). CONCLUSIONS: NSIPPV appears to be superior to NCPAP for the treatment of apnea in preterm infants.
2011 Vol. 13 (10): 783-786 [Abstract] ( 5139 ) [HTML 1KB] [PDF 918KB] ( 1683 )
787 XIA Li-Na, WANG Zhi-Qiang, WANG Zong-Min, ZHANG Pu
Expression of c-kit and Cx43 in neonates with spontaneous gastric perforation
OBJECTIVE: To study the clinical significance of interstitial cell of Cajal (ICC) in spontaneous neonatal gastric perforation by examining the expression of c-kit and Cx43 in neonates with this disorder. METHODS: The gastric specimens of 19 cases of neonatal gastric perforation from 2001 to 2010 and 8 cases of accidental death without digestive tract malformations (control) were collected. Immunohistochemical staining was employed to examine the expression of c-kit and Cx43 (immunomarkers of ICCs) in gastric tissues. RESULTS: The muscular layer of the stomach wall became thinner or deficient in the gastric perforation group. C-kit and Cx43 positive cells in gastric tissues decreased significantly in the gastric perforation group compared with those in the control group (P＜0.01). CONCLUSIONS: The development of spontaneous neonatal gastric perforation is associated with the decreased quantity of ICCs and damaged gap junction structure of the stomach wall.
2011 Vol. 13 (10): 787-789 [Abstract] ( 4951 ) [HTML 1KB] [PDF 1049KB] ( 1752 )
790 CHAO Shuang, ZENG Chao-Mei, LIU Jie
Thrombocytopenia in pregnancy and neonatal outcomes
OBJECTIVE: To study the relationship between thrombocytopenia in pregnancy associated with various causes and neonatal outcomes. METHODS: Medical records of 140 pregnant women with thrombocytopenia in pregnancy and the neonatal outcomes from January 2009 to December 2010 were reviewed retrospectively. The pregnant women were classified into four groups according to the causes of thrombocytopenia: gestational thrombocytopenia (GT; n=94), pregnancy with immune thrombocytopenic purpura (ITP; n=30), pregnancy with other hematological disease (aplastic anemia or myelodysplastic syndrome; n=12), and other causes (n=4): pregnancy induced hypertension syndrome, pregnancy with systemic lupus erythematosus, and pregnancy with alcoholic cirrhosis. The neonatal outcomes in the four groups were compared. RESULTS: The premature birth rates in the GT and the ITP groups were 11.3% and 16.7%, respectively. There was no significant difference between the two groups. The premature birth rate in the other hematological disease group was 53.8%, which was significantly higher than that in the GT (P<0.01) and the ITP groups (P<0.05). Congenital passive immune thrombocytopenia was found in 2 neonates (2%) in the GT group and in 4 neonates (13%) in the ITP group (P<0.05). In addition, other diseases were also observed in neonates in the ITP group, including 1 case (3%) of ITP and 1 case (3%) of Evans syndrome. Intracranial hemorrhage occurred in one neonate (8%) in the other hematological disease group. Neonatal lupus syndrome was found in 1 case (25%) in the other causes group. CONCLUSIONS: Thrombocytopenia in pregnancy associated with different causes may result in different neonatal outcomes.
2011 Vol. 13 (10): 790-793 [Abstract] ( 5929 ) [HTML 1KB] [PDF 918KB] ( 2705 )
794 LIANG Zhen-Zhen, ZHU Peng, GAO Rong, LU Ying, HUANG Wen, TAO Fang-Biao
Impacts of gestational weight gain on offspring weight and obesity: a 2-year follow up study
OBJECTIVE: To evaluate the impacts of maternal weight gain during pregnancy on offspring weight and obesity from birth to 24 months of age. METHODS: The information on maternal pre-pregnancy body mass index (BMI), gestational weight gain and demographic characteristics were collected from 317 pregnant women. The information on offspring weight, BMI and breastfeeding data was obtained from various follow-up examinations from 0 to 24 months of age. RESULTS: The logistic regression analysis showed that excessive gestational weight gain resulted in an increased risk of obesity in children at age of 6 months (adjusted RR=3.56, 95% CI:1.31-8.35) and 9 months (adjusted RR=2.87, 95% CI: 1.04-3.28) after adjustment for potential confounding factors. The linear regression model showed that there were significant correlations between gestational weight gain and Z score of weight in offsprings at birth (β=0.032, 95% CI: 0.008-0.057), 3 months (β=0.037, 95% CI: 0.013-0.062), 6 months (β=0.043, 95% CI: 0.017-0.068), 9 months (β=0.038, 95% CI: 0.013-0.063) and 12 months (β=0.034, 95% CI: 0.009-0.059), but not at 18 months and 24 months. CONCLUSIONS: Excessive gestational weight gain may affect offspring weight and increase the risk of obesity in children from birth to 12 months of age. During their second year of life, this effect will temporarily disappear.
2011 Vol. 13 (10): 794-798 [Abstract] ( 5351 ) [HTML 1KB] [PDF 931KB] ( 1494 )
799 XING Jun-Wei, LIU Ya-Hong, Bilal Haider SHAMSI, LIU Xiao-Hong, TAN Lu, XU Man
PMP22 mutation of an infant-onset Charcot-Marie-Tooth disease family
OBJECTIVE: To study the mutation of PMP22 gene of an early-onset family with Charcot-Marie-Tooth disease (CMT) and the genetic features of the disease. METHODS: Two patients with CMT, fifteen unaffected members in the family and 20 healthy controls were enrolled. STR-PCR and gene scanning were used to detect PMP22 duplication mutation. RESULTS: The mutations of PMP22 were found in the two patients and other five unaffected members in the family. The mutations were located in the STR locus D17S921 in 5 cases and in the STR locus D17S4A in 2 cases. The other members in the family and 20 healthy controls did not show the mutations of PMP22. CONCLUSIONS: The gene causing CMT in the family is found in the 17p11.2-p12 region containing PMP22 gene duplication mutation, resulting in the subtype CMT1A.
2011 Vol. 13 (10): 799-803 [Abstract] ( 5857 ) [HTML 1KB] [PDF 1262KB] ( 2000 )
804 WANG Li-Bo, MA Hong-Wei, WANG Lin, TIAN Xiao-Bo, HU Man, REN Shuang, TAN Ying-Hua
Relationship between gene mutations and intelligence in children with Duchenne muscular dystrophy
OBJECTIVE: To study the level of intelligence in children with Duchenne muscular dystrophy (DMD), and the relationship between the level of intelligence and gene mutations. METHODS: One hundred and two children with DMD between January 2009 and March 2011 were enrolled. DMD gene detection was performed through the multiplex ligation-dependent probe amplification (MLPA) in 84 cases. The level and the structure of intelligence were evaluated by Chinese Wechsler Intelligence Scale for Children (C-WISC) in 50 children with DMD (≥6 years old; DMD group) and in 50 age-and gender-matched healthy children (control group). RESULTS: The average intelligence quotient (IQ) was 84±21 in 102 children with DMD. Thirty patients (29.4%) had the full intelligence quotient (FIQ) less than 70. The FIQ, verbal intelligence quotient (VIQ), performance intelligence quotient (PIQ) and the scores of 11 sub-tests of intelligence in the DMD group were significantly lower than those in the control group (P<0.01). The IQ in patients with gene mutations at exon 56-79 was the lowest (59.3±11.9), followed by in patients with gene mutations at exon 45-55 (88.6±1.9), at exon 1-29 (97.5±9.6) and at exon 30-44 (102.8±3.8) (P<0.01). CONCLUSIONS: The FIQ, VIQ and PIQ in children with DMD are lower than those in healthy children. There is association between mental retardation and gene mutations.
2011 Vol. 13 (10): 804-807 [Abstract] ( 5588 ) [HTML 1KB] [PDF 952KB] ( 1718 )
808 PANG Huan-Xiang, QIAO Hong-Mei, CHENG Huan-Ji, ZHANG Yun-Feng, LIU Xiao-Jun, LI Jun-Zhao
Levels of TNF-α, IL-6 and IL-10 in bronchoalveolar lavage fluid in children with Mycoplasma pneumoniae pneumonia
OBJECTIVE: To study the levels and roles of cytokines TNF-α, IL-6 and IL-10 in bronchoalveolar lavage fluid (BALF) in children with Mycoplasma pneumoniae pneumonia (MPP). METHODS: The levels of TNF-α, IL-6 and IL-10 in BALF were measured using ELISA in children with MPP at acute stage (n=45) and at remission stage (n=30). Twenty children without lung lesions severed as the control group. RESULTS: The TNF-α, IL-6 and IL-10 levels in BALF were higher in children with MPP at acute stage than those in the control group (P<0.05). The levels of TNF-α and IL-6 in BALF at remission stage were reduced to the levels similar to the control group and were significantly lower than those at the acute stage in children with MPP. However, the levels of IL-10 in BALF remained at higher levels at remission stage in children with MPP. CONCLUSIONS: The levels of TNF-α, IL-6 and IL-10 in BALF increase in children with MPP at acute stage, suggesting that the cytokines may be involved in the pathogenesis of MPP.
2011 Vol. 13 (10): 808-810 [Abstract] ( 5912 ) [HTML 1KB] [PDF 908KB] ( 1935 )
811 ZHANG Hai-Yan, LU Min, LIU He-Lin, GAO Qiong, LIU Yi-Hong
Correlation of the stress reaction and temperament in children with bronchial asthma
OBJECTIVE: To study the correlation between the temperament dimension and the stress reaction in children with bronchial asthma. METHODS: Between February 2008 and May 2010, two hundred and twenty children with bronchial asthma and aged from 3-7 years were enrolled (asthma group). Eighty children without asthma, respiratory tract infections, serious body diseases and mental disorders who were age- and gender-matched to the asthma group served as the control group. The Carry temperament rating scales were filled by the children′s parents from the asthma and control groups. The serum levels of cortisol at 8am and 4pm and nerve growth factor (NGF) were measured by immunochemiluminescent assays. RESULTS: The scores of the rhythmicity and persistence in temperament dimensions in the asthma group were significantly lower than those in the control group; in contrast, the scores of phobotaxis, response intensity, emotion entity, adaptability and attention dispersion in the asthma group were significantly higher than those in the control group (P<0.05). The levels of serum cortisol at 8am and 4pm were lower, while the serum level of NGF was higher in the asthma group than those in the control group (P<0.05). The scores of response intensity, emotion entity and adaptability in temperament dimensions were positively correlated with serum NGF level (r=0.348, 0.468, 0.195, respectively; P<0.05), and inversely correlated with serum cortisol level at 8 am (r=-0.297, -0.462, -0.152, respectively; P<0.05) in the asthma group. CONCLUSIONS: The temperament dimension is associated with the stress reaction in children with bronchial asthma. This provides a basis for the treatment of bronchial asthma by neuropsychological adjustment.
2011 Vol. 13 (10): 811-813 [Abstract] ( 5084 ) [HTML 1KB] [PDF 904KB] ( 1399 )
814 PAN Qiong, DING Mei-Fang, ZHANG Shu, NING Ying, LIU Hong-Wei, WEI Hong, YUE Hong-Ni
Measurement of plasma mast cell carboxypeptidase and chymase levels in children with allergic diseases
OBJECTIVE: To evaluate the roles of plasma mast cell carboxypeptidase and chymase in the diagnosis of allergic diseases by measuring the contents of both in children. METHODS: A total of 59 children with allergic diseases and 53 healthy children were recruited into the study. Plasma levels of mast cell carboxypeptidase and chymase were measured using ELISA. RESULTS: The plasma levels of mast cell carboxypeptidase and chymase in children with allergic children were 1.089±0.752 ng/mL and 0.905(0.375-2.318) ng/mL, respectively, which were significantly higher than those in healthy children ［0.593±0.380 ng/mL and 0.454 (0.097-1.077) ng/mL respectively; P<0.05］.There was a significantly positive correlation between plasma mast cell carboxypeptidase and chymase levels in children with allergic diseases (r=0.684, P<0.01). CONCLUSIONS: Plasma levels of mast cell carboxypeptidase and chymase increase in children with allergic diseases, suggesting that mast cell carboxypeptidase and chymase may serve as the indexes for the diagnosis of allergic diseases.
2011 Vol. 13 (10): 814-816 [Abstract] ( 4995 ) [HTML 1KB] [PDF 903KB] ( 1348 )
817 WANG Jian-Jun, HUANG Yue, WU Chun, XU Yu-Xia, WEI Yu-Shu, ZHAO Yao
Levels of serum acylation-stimulating protein and C3 in children with primary nephrotic syndrome
OBJECTIVE: To study serum acylation-stimulating protein (ASP) and complement 3 (C3) levels and their relationship with blood lipids in children with primary nephrotic syndrome (PNS). METHODS: Blood samples were obtained from 35 PNS children with proteinuria, 25 children with PNS at remission and 35 body mass index-matched healthy children (control group). Fasting serum ASP, C3, albumin and blood lipids were measured. RESULTS: The serum ASP levels in the proteinuria group (101±22 nmol/L) were significantly higher than those in the remission group (32±8 nmol/L) and the control group (33± 8 nmol/L) (P0.05). CONCLUSIONS: The increased serum ASP level may be associated with a complemental mechanism against hyperlipidemia in children with PNS.
2011 Vol. 13 (10): 817-819 [Abstract] ( 4988 ) [HTML 1KB] [PDF 1005KB] ( 1324 )
820 CAI Hai-Fang, LAN Ju-Hong, QIAN Li-Jun
Application of clinical pathways in children with Rotavirus enteritis
OBJECTIVE: Clinical pathways are standardized, multidisciplinary, integrated management plans. This study aimed to evaluate the efficacy of clinical pathways in children with Rotavirus enteritis. METHODS: Seventy-one children with Rotavirus enteritis were treated according to the clinical pathways. Seventy-five children with Rotavirus enteritis who received routine therapy and nursing interventions served as the control group. The clinical efficacies were compared between the two groups. RESULTS: The average hospitalization duration was shortened, the hospitalization costs were reduced and the parents′ satisfaction rate increased in the observed group compared with the control group (P<0.05). CONCLUSIONS: The use of clinical pathways may decrease the hospitalization duration and costs and improve the quality of nursing care and the parents′ satisfaction rate in children with Rotavirus enteritis.
2011 Vol. 13 (10): 820-822 [Abstract] ( 4841 ) [HTML 1KB] [PDF 896KB] ( 1920 )
EXPERIMENTAL RESEARCH
823 GAO Jin-Zhi, ZHENG Rui-Dan, WANG Cheng-Bin, YING Yan-Qin, LUO Xiao-Ping
Effects of PI3K inhibitor LY294002 on the differentiation of mouse preadipocytes and the expression of C/EBPα and PPARγ
OBJECTIVE: This study examined the effects of PI3K inhibitor LY294002 on the differentiation of mouse preadipocytes and the expression of CCAAT enhancer binding protein α (C/EBPα) and peroxisome proliferation activated receptor γ (PPARγ), in order to study the possible roles of insulin receptor substrate (IRSs)/PI3K signal pathway in the differentiation of preadipocytes. METHODS: The mouse 3T3-L1 cells were cultured normally and divided into experimental and control groups. 3T3-L1 cells in the experimental group were treated with PI3K inhibitor LY294002 (25 μmol/L) and those in the control group were treated with DMSO culture medium. 3-isobutyl-1-methylxanthine (IBMX) (0.5 mmol/L), dexamethasone (10-6 mol/L) and insulin (5 μg/mL) were used to induce the differentiation of 3T3-L1 preadipocytes in both groups. Before culture, and 2, 4 and 8 days after culture, the cells were collected to detect the expression of C/EBPα and PPARγ by real-time PCR and Western blot assays. The lipid droplets of 3T3-L1 preadipocytes were observed by oil-red O staining. RESULTS: PI3K inhibitor LY294002 did not affect the expression of C/EBPα and PPARγ in un-induced 3T3-L1 preadipocytes (P>0.05), but decreased the expression of C/EBPα and PPARγ during the in vitro induced differentiation of 3T3-L1 preadipocytes compared with the control group (P<0.05 or 0.01). The lipid droplets count was greatly reduced by LY294002. CONCLUSIONS: PI3K inhibitor LY294002 can inhibit the differentiation of mouse 3T3-LI preadipocytes and the expression of C/EBPα and PPARγ in the differentiation of 3T3-LI preadipoeytes, suggesting that IRSs/PI3K signal pathway may play an important role in the differentiation of 3T3-L1 preadipocytes by regulating the expression of C/EBPα and PPARγ.
2011 Vol. 13 (10): 823-826 [Abstract] ( 6321 ) [HTML 1KB] [PDF 1021KB] ( 1894 )
827 LI Wen-Kai, LI Yun, ZHONG Li-Li
Effects of all-trans retinoic acid on airway responsiveness and airway remodeling in rats with asthma
OBJECTIVE: To study the effects of all-trans retinoic acid (ATRA) on airway responsiveness, airway remodeling and expression of matrix metalloproteinase-9 (MMP-9) protein in rats with asthma. METHODS: Forty rats were randomly divided into five groups: asthma model, normal saline (control), ATRA treatment, cotton oil treatment and budesonide treatment (n=8 each). Asthma was induced by ovalbumin sensitization and challenge in the asthma model, and the ATRA, cotton oil or budesonide treatment groups. ATRA (50 μg/kg), cotton oil (1 mL) or budesonide (0.32 mg/kg) was administered before ovalbumin challenge in the three treatment groups. Airway responsiveness was assessed. The lung tissues were sampled to detect airway remodeling and the expression of MMP-9 protein by immunohistochemistry. RESULTS: The expression of MMP-9 in lung tissues in the ATRA treatment group was significantly higher than that in the control group, but the airway responsiveness in the ATRA treatment group was not significantly different from that in the control group. The airway responsiveness and the expression of MMP-9 in lung tissues were significantly reduced in the ATRA treatment group compared with the asthma model group. The airway remodeling was significantly improved in the ATRA treatment group compared with the asthma model group. CONCLUSIONS: ATRA may alleviate airway hyperresponsiveness and airway remodeling possibly through decreasing the protein expression of MMP-9 in rats with asthma.
2011 Vol. 13 (10): 827-831 [Abstract] ( 4510 ) [HTML 1KB] [PDF 1668KB] ( 1173 )
837 ZHAO Hai-Yan, CHEN Hong-Wu, QIAN Xin-Hua, HU Xu-Chu
Preparation of polyclonal antibody of recombinant human thioredoxin-1 and its protective effects on neonatal rats with endotoxemia
OBJECTIVE: To clone the gene human thioredoxin 1 (hTrx-1) expressing its protein in the E.coli expression system and to obtain its polyclonal antibody, and to study the protective effects of hTrx-1 on neonatal rats with endotoxemia. METHODS: DNA encoding hTrx-1 from fetal liver cells was isolated by RT-PCR. The hTrx-1 was cloned to the prokaryotic expression plasmid PET-28a to induce its protein expression in the E.coli expression system. The purified hTrx-1 was injected into rats to prepare polyclonal antibody. Newborn Sprague-Dawley rats were randomly assigned to three groups: control, lipopolysaccharide (LPS) and hTrx-1 (n=12 each). The control and the LPS groups were intraperitoneally injected with normal saline and LPS (5 mg/kg), respectively. The hTrx-1 group received an intraperitoneal injection of hTrx-1 (10 mg/kg) 30 minutes before LPS injection. The mortality rate 24 hrs after injection was compared between the three groups. RESULTS: The prokaryotic expression plasmid PET-28a-hTrx-1 was constructed. The hTrx-1 protein was expressed and purified. The polyclonal antibody of hTrx-1 with the titer of 1∶51200 was prepared. The mortality rate of the control, LPS and hTrx-1 groups was 0, 67% and 17%, respectively (χ2=14.400, P<0.01). CONCLUSIONS: The polyclonal antibody of hTrx-1 is prepared successfully. The hTrx-1 protein has protective effects on neonatal rats with endotoxiamia.
2011 Vol. 13 (10): 837-841 [Abstract] ( 4970 ) [HTML 1KB] [PDF 1037KB] ( 1439 )
CLINICAL EXPERIENCE
842 WU Hua-Ping, ZHONG Jian-Min, CHEN Yong, YU Xiong-Ying
Value of electroencephalogram monitoring in the treatment of status epilepticus in children
No abstract available
2011 Vol. 13 (10): 842-844 [Abstract] ( 4445 ) [HTML 1KB] [PDF 907KB] ( 1235 )
845 YOU Chu-Ming, FU Wan-Hai, QIN Xiao-Fei, ZHANG Yu, YU Qiu-Min
Clinical trail of high frequency oscillatory ventilation in the treatment of respiratory distress syndrome in newborns who did not respond to conventional mechanical ventilation
No abstract available
2011 Vol. 13 (10): 845-847 [Abstract] ( 4668 ) [HTML 1KB] [PDF 903KB] ( 1643 )
CASE REPORT
848 LI Yu-Mei, LI Nan, XU Wei, YAN Chao-Ying
Systemic mastocytosis in a neonate
No abstract available
2011 Vol. 13 (10): 848-849 [Abstract] ( 3904 ) [HTML 1KB] [PDF 990KB] ( 1472 )
850 ZHONG Yan-Lan, DANG Xi-Qiang
Hypereosinophilic syndrome involving nephritic syndrome: a case report
No abstract available
2011 Vol. 13 (10): 850-851 [Abstract] ( 4518 ) [HTML 1KB] [PDF 885KB] ( 1457 )
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