Association between ADRB2 regulatory region polymorphisms and susceptibility to childhood asthma

CAI Ming-Xuan; WEI Bing; LIAO Shi-E; FU Jin-Yue; LIU Ya-Jun; LI Ling-Xue

doi:10.7499/j.issn.1008-8830.2108054

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Chinese Journal of Contemporary Pediatrics ›› 2021, Vol. 23 ›› Issue (11) : 1132-1140. DOI: 10.7499/j.issn.1008-8830.2108054

CLINICAL RESEARCH

Association between ADRB2 regulatory region polymorphisms and susceptibility to childhood asthma

CAI Ming-Xuan, WEI Bing, LIAO Shi-E, FU Jin-Yue, LIU Ya-Jun, LI Ling-Xue

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Abstract

Objective To study the association of β2-drenergic receptor (ADRB2) regulatory region single nucleotides polymorphism (SNP)/haplotypes at rs11168070, rs17108803, rs2053044, rs12654778, rs11959427, and rs2895795 loci with childhood asthma. Methods A total of 143 children with asthma who attended the hospital from October 2016 to October 2020 were enrolled as the asthma group, among whom 61 children had mild symptoms (mild group) and 82 children had moderate-to-severe symptoms (moderate-to-severe group). A total of 137 healthy children were enrolled as the control group. Peripheral venous blood samples were collected from the two groups. The SNaPshot SNP technique was used to analyze the SNP and haplotypes of the ADRB2 regulatory region at rs11168070, rs17108803, rs2053044, rs12654778, rs11959427, and rs2895795 loci in all children. The asthma group and the control group were compared in terms of the association of ADRB2 regulatory region SNP and haplotypes at the above six loci with susceptibility to asthma and severity of asthma. Results Polymorphisms were observed in the ADRB2 regulation region at the above six loci in both the asthma group and the control group, with significant differences between the two groups in the distribution of genotype and allele frequencies at rs2895795 (-1429T /A), rs2053044(-1023G/A), and rs12654778 (-654G/A) loci (P<0.05). Linkage disequilibrium of SNP was observed at the six loci of the ADRB2 regulatory region.The haplotypes of TATGCT, TATGGC, and AGTGCT were associated with susceptibility to childhood asthma, among which TATGCT and TATGGC were risk factors for childhood asthma (OR=1.792 and 1.946 respectively, P<0.05), while AGTGCT was a protective factor (OR=0.523, P<0.05). Conclusions SNP/haplotype of the ADRB2 regulatory region is associated with the susceptibility to childhood asthma. The haplotypes of TATGCT and TATGGC formed by such SNP/haplotype are risk factors for childhood asthma, while AGTGCT is a protective factor.

Key words

Asthma / ADRB2 gene / Susceptibility / Single nucleotide polymorphism / Haplotype / Child

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CAI Ming-Xuan, WEI Bing, LIAO Shi-E, FU Jin-Yue, LIU Ya-Jun, LI Ling-Xue. Association between ADRB2 regulatory region polymorphisms and susceptibility to childhood asthma[J]. Chinese Journal of Contemporary Pediatrics. 2021, 23(11): 1132-1140 https://doi.org/10.7499/j.issn.1008-8830.2108054

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