Clinical value of metagenomic next-generation sequencing in complicated infectious diseases

HAN Si-Yu

doi:10.7499/j.issn.1008-8830.2110064

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Chinese Journal of Contemporary Pediatrics ›› 2022, Vol. 24 ›› Issue (2) : 210-215. DOI: 10.7499/j.issn.1008-8830.2110064

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Clinical value of metagenomic next-generation sequencing in complicated infectious diseases

HAN Si-Yu

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Abstract

Infectious diseases are commonly seen in clinical practice, and pathogen diagnosis is the key link in diagnosis and treatment; however, conventional pathogen detection methods cannot meet clinical needs due to time-consuming operation and low positive rate. As a new pathogen detection method, metagenomic next-generation sequencing (mNGS) has a wide detection range and can detect bacteria, viruses, fungi, parasites, rare pathogens, and even unknown pathogens. The technique of mNGS is unbiased and can rapidly, efficiently, and accurately obtain all nucleic acid information in test samples, analyze pathogens, and guide clinical diagnosis and treatment, thereby playing an important role in complicated infectious diseases. This article reviews the diagnostic advantages and clinical value of mNGS in bacterial, fungal, viral, and parasitic infections.

Key words

Infectious disease / Metagenomic next-generation sequencing / Pathogen detection / Child

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HAN Si-Yu. Clinical value of metagenomic next-generation sequencing in complicated infectious diseases[J]. Chinese Journal of Contemporary Pediatrics. 2022, 24(2): 210-215 https://doi.org/10.7499/j.issn.1008-8830.2110064

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