Serum vitamin K2 level and its association with bone metabolism markers in 1 732 children

DU Chang-Xiu; LI Na

doi:10.7499/j.issn.1008-8830.2205090

PDF(574 KB)

Chinese Journal of Contemporary Pediatrics ›› 2022, Vol. 24 ›› Issue (10) : 1130-1135. DOI: 10.7499/j.issn.1008-8830.2205090

CLINICAL RESEARCH

Serum vitamin K₂ level and its association with bone metabolism markers in 1 732 children

DU Chang-Xiu, LI Na

Author information +

History +

Abstract

Objective To study the level of serum vitamin K₂ (VitK₂) and its association with bone metabolism markers osteocalcin (OC), type I procollagen amino-terminal peptide (PINP), and type I collagen carboxy-terminal peptide (CTX) in children. Methods A prospective analysis was performed on 1 732 children who underwent routine physical examination from October 2020 to October 2021. The serum levels of VitK₂ and 25-hydroxy vitamin D [25(OH)D] were measured. According to age, they were divided into four groups: <1 year, 1-3 years group, >3-6 years group, and >6-14 years. A total of 309 children with 25(OH)D≥50 nmol/L were screened out, and serum levels of OC, PINP, and CTX were measured to investigate the correlation of the serum levels of OC, PINP, and CTX with serum VitK₂ levels in different age groups. Results The prevalence rate of serum VitK₂ deficiency was 52.31% (906/1 732). The VitK₂ deficiency group had higher prevalence rates of overweight/obesity and growth pain (≥3 years of age) than the normal VitK₂ group (P<0.05). There were differences in the prevalence rate of serum VitK₂ deficiency (P<0.0083) and the serum level of VitK₂ (P<0.05) between the 1-3 years group and the >6-14 years group. The <1 year group had a higher serum level of CTX and a lower serum level of PINP than the >3-6 years group and the >6-14 years group (P<0.05). The <1 year group had a lower serum level of OC than the >6-14 years group (P<0.05). Serum VitK₂ level was positively correlated with OC level (r_s=0.347, P<0.01), and CTX level was negatively correlated with PINP level (r_s=-0.317, P<0.01). Conclusions Serum VitK₂ deficiency may be associated with overweight/obesity. Serum VitK₂ may affect the level of OC and even bone health.

Key words

Vitamin K₂ / Osteocalcin / Type I procollagen amino-terminal peptide / Type I collagen carboxy-terminal peptide / Child

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

DU Chang-Xiu, LI Na. Serum vitamin K₂ level and its association with bone metabolism markers in 1 732 children[J]. Chinese Journal of Contemporary Pediatrics. 2022, 24(10): 1130-1135 https://doi.org/10.7499/j.issn.1008-8830.2205090

References

1 Zhang Z, Liu L, Liu C, et al. New aspects of microbial vitamin K2 production by expanding the product spectrum[J]. Microb Cell Fact, 2021, 20(1): 84. PMID: 33849534. PMCID: PMC8042841. DOI: 10.1186/s12934-021-01574-7.
2 Sato T, Inaba N, Yamashita T. MK-7 and its effects on bone quality and strength[J]. Nutrients, 2020, 12(4): 965. PMID: 32244313. PMCID: PMC7230802. DOI: 10.3390/nu12040965.
3 Nazifova-Tasinova NF, Atanasov AA, Pasheva MG, et al. Circulating uncarboxylated matrix Gla protein in patients with atrial fibrillation or heart failure with preserved ejection fraction[J]. Arch Physiol Biochem, 2020. Epub ahead of print. PMID: 32620059. DOI: 10.1080/13813455.2020.1786130.
4 Miura M, Satoh Y. Significance of bone turnover marker measurement in the treatment of osteoporosis[J]. Yakugaku Zasshi, 2019, 139(1): 27-33. PMID: 30606924. DOI: 10.1248/yakushi.18-00154-3.
5 van Summeren MJ, Braam LA, Lilien MR, et al. The effect of menaquinone-7 (vitamin K2) supplementation on osteocalcin carboxylation in healthy prepubertal children[J]. Br J Nutr, 2009, 102(8): 1171-1178. PMID: 19450370. DOI: 10.1017/S0007114509382100.
6 Weaver CM, Gordon CM, Janz KF, et al. The National Osteoporosis Foundation's position statement on peak bone mass development and lifestyle factors: a systematic review and implementation recommendations[J]. Osteoporos Int, 2016, 27(4): 1281-1386. PMID: 26856587. PMCID: PMC4791473. DOI: 10.1007/s00198-015-3440-3.
7 陈淑玲, 赵瑾珠, 郝燕. 维生素K与儿童骨健康的研究进展[J]. 中国儿童保健杂志, 2021, 29(7): 742-745. DOI: 10.11852/zgetbjzz2020-1085.
8 胡雪松, 覃佳强, 郭彬, 等. 维生素K治疗儿童废用性骨质疏松的疗效观察[J]. 西部医学, 2018, 30(5): 704-706. DOI: 10.3969/j.issn.1672-3511.2018.05.019.
9 李辉, 季成叶, 宗心南, 等. 中国0～18岁儿童、青少年体块指数的生长曲线[J]. 中华儿科杂志, 2009, 47(7): 493-498. PMID: 19951508. DOI: 10.3760/cma.j.issn.0578-1310.2009.07.004.
10 Ross AC, Manson JE, Abrams SA, et al. The 2011 report on dietary reference intakes for calcium and vitamin D from the Institute of Medicine: what clinicians need to know[J]. J Clin Endocrinol Metab, 2011, 96(1): 53-58. PMID: 21118827. PMCID: PMC3046611. DOI: 10.1210/jc.2010-2704.
11 Mandatori D, Pelusi L, Schiavone V, et al. The dual role of vitamin K2 in "bone-vascular crosstalk": opposite effects on bone loss and vascular calcification[J]. Nutrients, 2021, 13(4): 1222. PMID: 33917175. PMCID: PMC8067793. DOI: 10.3390/nu13041222.
12 Duan F, Mei C, Yang L, et al. Vitamin K2 promotes PI3K/AKT/HIF-1α-mediated glycolysis that leads to AMPK-dependent autophagic cell death in bladder cancer cells[J]. Sci Rep, 2020, 10(1): 7714. PMID: 32382009. PMCID: PMC7206016. DOI: 10.1038/s41598-020-64880-x.
13 Sogabe N, Maruyama R, Baba O, et al. Effects of long-term vitamin K1 (phylloquinone) or vitamin K2 (menaquinone-4) supplementation on body composition and serum parameters in rats[J]. Bone, 2011, 48(5): 1036-1042. PMID: 21295170. DOI: 10.1016/j.bone.2011.01.020.
14 Klapkova E, Cepova J, Dunovska K, et al. Determination of vitamins K1, MK-4, and MK-7 in human serum of postmenopausal women by HPLC with fluorescence detection[J]. J Clin Lab Anal, 2018, 32(5): e22381. PMID: 29333616. PMCID: PMC6816853. DOI: 10.1002/jcla.22381.
15 周鹏丽, 陈杰鹏, 段丽丽, 等. 维生素K2的临床检测方法及其评价指标[J]. 中国微生态学杂志, 2021, 33(3): 365-368. DOI: 10.13381/j.cnki.cjm.202103024.
16 Caluwé R, Verbeke F, De Vriese AS. Evaluation of vitamin K status and rationale for vitamin K supplementation in dialysis patients[J]. Nephrol Dial Transplant, 2020, 35(1): 23-33. PMID: 30590803. DOI: 10.1093/ndt/gfy373.
17 Zhang Y, Bala V, Mao Z, et al. A concise review of quantification methods for determination of vitamin K in various biological matrices[J]. J Pharm Biomed Anal, 2019, 169: 133-141. PMID: 30861405. PMCID: PMC6496949. DOI: 10.1016/j.jpba.2019.03.006.
18 Marles RJ, Roe AL, Oketch-Rabah HA. US Pharmacopeial Convention safety evaluation of menaquinone-7, a form of vitamin K[J]. Nutr Rev, 2017, 75(7): 553-578. PMID: 28838081. DOI: 10.1093/nutrit/nux022.
19 Ozdemir MA, Yilmaz K, Abdulrezzak U, et al. The efficacy of vitamin K2 and calcitriol combination on thalassemic osteopathy[J]. J Pediatr Hematol Oncol, 2013, 35(8): 623-627. PMID: 24136015. DOI: 10.1097/MPH.0000000000000040.
20 周建烈, 徐峰, 吴斯婷, 等. 《美国药典委员会对MK-7(维生素K2)安全性评价》的解读[J]. 沈阳药科大学学报, 2019, 36(4): 361-368. DOI: 10.14066/j.cnki.cn21-1349/r.2019.04.014.
21 洪维, 朱汉民, 程群, 等. 血清维生素D水平与骨代谢状态的相关性: 附1389例观察[J]. 中华骨质疏松和骨矿盐疾病杂志, 2011, 4(4): 224-231. DOI: 10.3969/j.issn.1674-2591.2011.04.002.
22 Thiering E, Brüske I, Kratzsch J, et al. Associations between serum 25-hydroxyvitamin D and bone turnover markers in a population based sample of German children[J]. Sci Rep, 2015, 5: 18138. PMID: 26667774. PMCID: PMC4678865. DOI: 10.1038/srep18138.
23 金轶. 不同年龄段小儿骨骼维生素K营养状况的评价与分析[J]. 中国全科医学, 2013, 16(24): 2880-2882. DOI: 10.3969/j.issn.1007-9572.2013.08.107.
24 康丽娟, 李宝强, 徐传伟. 维生素K对营养性维生素D缺乏性佝偻病患儿骨钙素羧化率的影响[J]. 儿科药学杂志, 2018, 24(10): 19-21. DOI: 10.13407/j.cnki.jpp.1672-108X.2018.10.006.
25 Nicolaidou P, Stavrinadis I, Loukou I, et al. The effect of vitamin K supplementation on biochemical markers of bone formation in children and adolescents with cystic fibrosis[J]. Eur J Pediatr, 2006, 165(8): 540-545. PMID: 16622660. DOI: 10.1007/s00431-006-0132-1.