Clinical practice of whole-genome sequencing in the rapid diagnosis of critically ill neonates

XIAO Fei-Fan; LU Yu-Lan; WU Bing-Bing; DONG Xin-Ran; CHENG Guo-Qiang; HU Li-Yuan; ZHOU Wen-Hao; PENG Xiao-Min; YANG Lin; WANG Hui-Jun

doi:10.7499/j.issn.1008-8830.2210096

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Chinese Journal of Contemporary Pediatrics ›› 2023, Vol. 25 ›› Issue (2) : 135-139. DOI: 10.7499/j.issn.1008-8830.2210096

CLINICAL RESEARCH

Clinical practice of whole-genome sequencing in the rapid diagnosis of critically ill neonates

XIAO Fei-Fan, LU Yu-Lan, WU Bing-Bing, DONG Xin-Ran, CHENG Guo-Qiang, HU Li-Yuan, ZHOU Wen-Hao, PENG Xiao-Min, YANG Lin, WANG Hui-Jun

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Abstract

Objective To explore the application of whole-genome sequencing (WGS) in the rapid clinical diagnosis of critically ill neonates. Methods The critically ill neonates who admitted to the neonatal intensive care unit of Children's Hospital of Fudan University and underwent WGS from August to September, 2019 were enrolled in this prospective study. The genetic testing results and clinical outcome were analyzed with reference to the sequencing data and clinical features of the neonates. Results A total of 15 neonates were tested, among whom there were 9 boys and 6 girls. The main reason for hospitalization included abnormal breathing in 7 neonates, poor response in 2 neonates, feeding difficulty in 2 neonates, fever in 1 neonate, hypothermia in 1 neonate, preterm birth in 1 neonate, and convulsion in 1 neonate. The mean turn-around time was 4.5 days for WGS. Finally a genetic diagnosis was obtained for 3 neonates, with a positive diagnostic rate of 20% (3/15). Among the 3 neonates, 2 neonates were withdrawn from the treatment due to severe conditions and 1 neonate died on the day when the sample was sent for genetic testing, whose etiology could be explained by the results of genetic testing. Conclusions WGS technique can provide a timely and effective diagnosis for critically ill neonates suspected of genetic diseases and provide genetic evidence for clinical treatment of critically ill cases.

Key words

Critical illness / Genetic disease / Whole-genome sequencing / Neonate

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XIAO Fei-Fan, LU Yu-Lan, WU Bing-Bing, DONG Xin-Ran, CHENG Guo-Qiang, HU Li-Yuan, ZHOU Wen-Hao, PENG Xiao-Min, YANG Lin, WANG Hui-Jun. Clinical practice of whole-genome sequencing in the rapid diagnosis of critically ill neonates[J]. Chinese Journal of Contemporary Pediatrics. 2023, 25(2): 135-139 https://doi.org/10.7499/j.issn.1008-8830.2210096

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