Association between HLA-DRB1 gene polymorphisms and genetic susceptibility of early-onset severe preeclampsia

LAI Chun-Chi; ZHANG Lu-Lu; SUN Meng-Ya; SUN Jun-Fang; JIANG Hong

doi:10.7499/j.issn.1008-8830.2303100

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Chinese Journal of Contemporary Pediatrics ›› 2023, Vol. 25 ›› Issue (10) : 1022-1027. DOI: 10.7499/j.issn.1008-8830.2303100

CLINICAL RESEARCH

Association between HLA-DRB1 gene polymorphisms and genetic susceptibility of early-onset severe preeclampsia

LAI Chun-Chi, ZHANG Lu-Lu, SUN Meng-Ya, SUN Jun-Fang, JIANG Hong

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Abstract

Objective To explore the correlation between the single nucleotide polymorphisms (SNP) of rs3135388, rs114293611 and rs142804168 of HLA-DRB1 gene and early-onset severe preeclampsia (sPE). Methods Blood samples were collected from 102 early-onset sPE mothers and their neonates (sPE group), as well as 120 normotensive mothers and their neonates (control group). Sanger sequencing was performed to compare the genotype distribution, allele frequencies, and differences in genotype distribution after maternal-infant compatibility between the two groups. Results Statistically significant differences in genotype distribution at rs114293611 of HLA-DRB1 gene were observed between sPE and control groups in both mothers and neonates (P<0.05). The frequency of the T allele at rs114293611 was higher in the sPE group of neonates than that in the control group (P<0.05), while no significant difference was found between the two groups of mothers (P>0.05). The maternal-infant genotype compatibility analysis showed significant differences in genotype distribution between sPE and control groups (P<0.05). There were no significant differences in genotype distribution and allele frequencies at rs3135388 and rs142804168 of HLA-DRB1 gene between the two groups of mothers and neonates (P>0.05). Conclusions The SNP at rs114293611 of HLA-DRB1 gene may be associated with the development of early-onset sPE in mothers. Maternal-infant genotype compatibility abnormality at rs114293611 of HLA-DRB1 gene may be a predisposition factor for the development of sPE.

Key words

Preeclampsia / HLA-DRB1 gene / Single nucleotide polymorphism / Pregnant women / Neonate

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LAI Chun-Chi, ZHANG Lu-Lu, SUN Meng-Ya, SUN Jun-Fang, JIANG Hong. Association between HLA-DRB1 gene polymorphisms and genetic susceptibility of early-onset severe preeclampsia[J]. Chinese Journal of Contemporary Pediatrics. 2023, 25(10): 1022-1027 https://doi.org/10.7499/j.issn.1008-8830.2303100

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