Expression of soluble factor-related apoptosis ligand in peripheral blood and microRNA-147b in monocytes in children with sepsis and their association with prognosis

ZHANG Jun; LIN Xiao-Fei; WU Yun-Duo; ZHU Hong-Li; LIU Juan

doi:10.7499/j.issn.1008-8830.2408152

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Chinese Journal of Contemporary Pediatrics ›› 2025, Vol. 27 ›› Issue (1) : 82-87. DOI: 10.7499/j.issn.1008-8830.2408152

CLINICAL RESEARCH

Expression of soluble factor-related apoptosis ligand in peripheral blood and microRNA-147b in monocytes in children with sepsis and their association with prognosis

ZHANG Jun, LIN Xiao-Fei, WU Yun-Duo, ZHU Hong-Li, LIU Juan

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Abstract

Objective To investigate the expression of soluble factor-related apoptosis ligand (sFasL) in peripheral blood and microRNA-147b (miR-147b) in monocytes in children with sepsis and their value in assessing prognosis. Methods A prospective study was conducted on 124 children with sepsis (sepsis group), 60 children with common infections (infection group), and 60 healthy children undergoing physical examinations (healthy control group). The independent risk factors for poor prognosis in children with sepsis were analyzed, and the value of serum sFasL and monocyte miR-147b in predicting poor prognosis in children with sepsis was assessed. Results The serum level of sFasL and the relative expression of miR-147b in monocytes were highest in the sepsis group, followed by the infection group and the healthy control group (P<0.05). The multivariate logistic regression analysis showed that the serum level of sFasL and the relative expression of miR-147b in monocytes were closely associated with the poor prognosis of children with sepsis (P<0.05). The receiver operating characteristic curve analysis showed that the combination of serum sFasL level and relative expression of miR-147b in monocytes had a larger area under the curve compared to each indicator alone in predicting the prognosis of children with sepsis (P<0.05). Conclusions There are significant increases in the level of sFasL in peripheral blood and the relative expression of miR-147b in monocytes in children with sepsis. The combined use of these two indicators has relatively high clinical value in assessing the prognosis of children with sepsis.

Key words

Sepsis / Soluble factor-related apoptosis ligand / Monocyte miR-147b / Prognosis / Child

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ZHANG Jun, LIN Xiao-Fei, WU Yun-Duo, ZHU Hong-Li, LIU Juan. Expression of soluble factor-related apoptosis ligand in peripheral blood and microRNA-147b in monocytes in children with sepsis and their association with prognosis[J]. Chinese Journal of Contemporary Pediatrics. 2025, 27(1): 82-87 https://doi.org/10.7499/j.issn.1008-8830.2408152

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