Clinical significance of neutrophil chemokines in intestinal tissue and related receptor expression in peripheral blood in neonatal necrotizing enterocolitis

Fan-Yue QIN; Hui-Fang DONG; Ya-Xuan LIU; Qing YAN; Li-Na HE; Zhe FU; Hui-Juan WANG; Fa-Lin XU

doi:10.7499/j.issn.1008-8830.2506017

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Chinese Journal of Contemporary Pediatrics ›› 2026, Vol. 28 ›› Issue (2) : 213-219. DOI: 10.7499/j.issn.1008-8830.2506017

CLINICAL RESEARCH

Clinical significance of neutrophil chemokines in intestinal tissue and related receptor expression in peripheral blood in neonatal necrotizing enterocolitis

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Abstract

Objective To analyze changes in neutrophil infiltration and chemokines in intestinal tissue from neonates with necrotizing enterocolitis (NEC), and to assess the expression of related receptors in peripheral blood, in order to explore their significance in NEC. Methods Clinical data, peripheral blood, and intestinal tissue from neonates with NEC and intestinal atresia who underwent surgery at the Third Affiliated Hospital of Zhengzhou University from June 2022 to September 2023 were prospectively collected and classified into an NEC group and an intestinal atresia group. Pathology results, chemokine levels in intestinal tissue, and neutrophil chemokine receptors in peripheral blood were compared between the two groups. Results In the NEC group, marked accumulation of neutrophils was observed in intestinal tissue. Compared with the intestinal atresia group, intestinal tissue concentrations of CCL2, CCL3, CCL4, CCL5, CXCL1, CXCL8, and interleukin-1 receptor antagonist were higher (all P<0.05). In peripheral blood leukocyte subsets, the percentages of cells co-expressing CXCR2/CCR1 and CD66/CXCR2 were higher in the NEC group (both P<0.05). Conclusions In NEC, chemokine concentrations in intestinal tissue are broadly elevated, and peripheral blood leukocytes express neutrophil chemokine receptors, which together may promote the migration and infiltration of neutrophils from peripheral blood into intestinal tissue.

Key words

Necrotizing enterocolitis / Neutrophil / Chemokine / Luminex / Flow cytometry / Neonate

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Fan-Yue QIN , Hui-Fang DONG , Ya-Xuan LIU , et al . Clinical significance of neutrophil chemokines in intestinal tissue and related receptor expression in peripheral blood in neonatal necrotizing enterocolitis[J]. Chinese Journal of Contemporary Pediatrics. 2026, 28(2): 213-219 https://doi.org/10.7499/j.issn.1008-8830.2506017

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