Effects of recombinant human erythropoietin on serum levels of neuron-specific enolase, S-100β protein and myelin basic protein in rats following status epilepticus

JIANG  Chun-Ming; DU  Jian-Min; LIU  Zhan-Li; CHEN  Li-Qiong; FENG  Mei; YANG  Yi-Hua; WANG  Jie-Hua; HUANG  Xian-Mei

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Chinese Journal of Contemporary Pediatrics ›› 2011, Vol. 13 ›› Issue (1) : 50-52.

Effects of recombinant human erythropoietin on serum levels of neuron-specific enolase, S-100β protein and myelin basic protein in rats following status epilepticus

JIANG Chun-Ming, DU Jian-Min, LIU Zhan-Li, CHEN Li-Qiong, FENG Mei, YANG Yi-Hua, WANG Jie-Hua, HUANG Xian-Mei

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Abstract

OBJECTIVE: This study examined the effect of recombinant human erythropoietin (r-HuEPO) on the serum levels of neuron-specific enolase (NSE), S-100β protein and myelin basic protein (MBP) in young rats 24 hrs after lithium-pilocarpine-induced status epilepticus (SE) in order to study the potential role of r-HuEPO in epileptic brain damage. METHODS: Forty 19-21-day-old male Sprague-Dawley (SD) rats were randomly divided into four groups (n=10): normal control group, SE, r-HuEPO pretreated-SE and r-HuEPO. SE was induced by lithium-pilocarpine. R-HuEPO (500 IU/kg) was intraperitoneally injected in the r-HuEPO pretreated-SE and r-HuEPO groups 4 hrs before SE. Serum levels of NSE, S-100β and MBP were determined 24 hrs after the SE event. RESULTS: Serum levels of NSE, S-100β and MBP in the SE group increased significantly compared with those in the normal control and the r-HuEPO groups (P＜0.05). The r-HuEPO pretreated-SE group showed significantly decreased serum levels of NSE, S-100β and MBP compared with the SE group (P＜0.05). CONCLUSIONS: r-HuEPO may reduce the expression of NSE, S-100β and MBP and thus might provide an early protective effect against epileptic brain injury.

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Status epilepticus / Neuron-specific enolase / S-100β protein / Erythropoietin / Rats

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JIANG Chun-Ming, DU Jian-Min, LIU Zhan-Li, CHEN Li-Qiong, FENG Mei, YANG Yi-Hua, WANG Jie-Hua, HUANG Xian-Mei. Effects of recombinant human erythropoietin on serum levels of neuron-specific enolase, S-100β protein and myelin basic protein in rats following status epilepticus[J]. Chinese Journal of Contemporary Pediatrics. 2011, 13(1): 50-52

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