FLT3 mutations in children with acute myeloid leukemia: a single center study

RUAN Min; WANG Ya-Qin; ZHANG Li; LIU Tian-Feng; LIU Fang; LIU Xiao-Ming; ZHANG Jia-Yuan; ZOU Yao; CHEN Yu-Mei; ZHU Xiao-Fan

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Chinese Journal of Contemporary Pediatrics ›› 2011, Vol. 13 ›› Issue (11) : 863-866.

CLINICAL RESEARCH

FLT3 mutations in children with acute myeloid leukemia: a single center study

RUAN Min, WANG Ya-Qin, ZHANG Li, LIU Tian-Feng, LIU Fang, LIU Xiao-Ming, ZHANG Jia-Yuan, ZOU Yao, CHEN Yu-Mei, ZHU Xiao-Fan

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Abstract

OBJECTIVE: To study the clinical significance of FMS-like tyrosine kinase 3 (FLT3) mutations including internal tandem duplication (ITD) mutation and point mutation of tyrosine kinase domain (TKD) in children with acute myeloid leukemia (AML). METHODS: Bone marrow samples from 116 children with newly-diagnosed AML were obtained．Gene mutations of FLT3/ITD and FLT3/TKD were detected by RT-PCR. The relationship of FLT3 gene mutations with the clinical characteristics and the therapeutic efficacy was observed. RESULTS: FLT3/ITD and FLT3/TKD mutations were detected in 9 cases (7.8%) and 13 cases (11.2%) respectively out of the 116 children. FLT3/ITD mutations were observed in 3 cases of AML-M3 (3/9; 33.3%) and in 3 cases of AML-M5 (3/9; 33.3%). FLT3/TKD mutations were the most common in AML-M3 patients (10/13; 76.9%). The patients with FLT3/ITD mutations had a significantly higher peripheral WBC count and marrow blast percentage compared with the patients without FLT3/ITD mutations at diagnosis (P<0.01). The 3-year overall survival rate in patients with FLT3/ITD mutations was significantly lower than that in patients without FLT3/ITD mutations (38.9% vs 64.3%; P<0.05).ConclusionsFLT3/TKD mutations are common in children with AML-M3. The AML children with FLT3/ITD mutations present a high peripheral WBC count and a high marrow blast percentage at diagnosis and have an unfavorable outcome.

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Acute myeloid leukemia / FLT3 / Gene mutation / Child

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RUAN Min, WANG Ya-Qin, ZHANG Li, LIU Tian-Feng, LIU Fang, LIU Xiao-Ming, ZHANG Jia-Yuan, ZOU Yao, CHEN Yu-Mei, ZHU Xiao-Fan. FLT3 mutations in children with acute myeloid leukemia: a single center study[J]. Chinese Journal of Contemporary Pediatrics. 2011, 13(11): 863-866

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