Correlation of renal AQP1-4 protein expression with renal parenchyma thickness and glomerular filtration rate in children with congenital hydronephrosis

LI Zhen-Zhen; WANG Zhi-Min; XING Lu; ZHANG Hong; WEN Jian-Guo

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Chinese Journal of Contemporary Pediatrics ›› 2011, Vol. 13 ›› Issue (11) : 878-882.

Correlation of renal AQP1-4 protein expression with renal parenchyma thickness and glomerular filtration rate in children with congenital hydronephrosis

LI Zhen-Zhen, WANG Zhi-Min, XING Lu, ZHANG Hong, WEN Jian-Guo

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Abstract

OBJECTIVE: To study the relationship of renal aquaporin -1, -2, -3, and -4 (AQP1-4) expression with renal parenchymal thickness and glomerular filtration rate (GFR) in children with congenital hydronephrotis. METHODS: Renal tissue samples were obtained from 10 kidneys of 10 children (age: 62.3±18.3 months) with hydronephrosis and who underwent Anderson-Hynes pyeloplasty. Renal control samples were obtained from 6 children (age: 62.7±17.1 months) undergoing nephrectomy for nephroblastoma and were confirmed histologically as normal renal tissues. Renal parenchymal thickness of the hydronephrotic kidneys was measured by ultrasound preoperatively and was verified at operation. Renal GFR was assessed using 99mTc-DTPA scintigraphy preoperatively. Western blot was used to examine the expression of AQP1-4 in the renal tissues. The correlations of renal AQP1-4 expression with the renal parenchymal thickness and GFR were assessed by Pearson correlation analysis. RESULTS: The expression of AQP1-4 in the hydronephrotis group was markedly reduced compared to that in the control group (P<0.05). The mean renal parenchymal thickness of the hydronephrotic kidney was 4.59±2.25 mm measured by ultrasound preoperatively. The mean GFR of the obstructed kidney was significantly lower than that of the contralateral kidney in the hydronephrosis group (40±12 mL/min vs 105±20 mL/min; P<0.05). The expression of AQP1, 2, 3 and 4 was positively correlated with preoperative renal GFR and renal parenchymal thickness in the hydronephrosis group (P<0.05). Renal parenchymal thickness was positively correlated with renal GFR (P<0.05). CONCLUSIONS: The expression of renal AQP1-4 is reduced in children with congenital hydronephrosis. The expression levels of AQP1-4 are positively correlated with renal parenchymal thickness and GFR.

Key words

Aquaporin / Congenital hydronephrosis / Renal parenchymal thickness / Glomerular filtration rate / Child

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LI Zhen-Zhen, WANG Zhi-Min, XING Lu, ZHANG Hong, WEN Jian-Guo. Correlation of renal AQP1-4 protein expression with renal parenchyma thickness and glomerular filtration rate in children with congenital hydronephrosis[J]. Chinese Journal of Contemporary Pediatrics. 2011, 13(11): 878-882

References

［1］Callen PW. Ultrasonography in obstetric and gynecology［M］. 4th ed. Philadelphia: W.B. Saunders Company, 2000: 7.

［2］文建国.新生鼠输尿管不全梗阻后肾盂压力和肾脏形态变化的观察［J］.中华小儿外科杂志， 2002，23（4）：344-347.

［3］文建国，王贵宪，李真珍.幼鼠双侧输尿管梗阻24h后肾脏水通道蛋白1-4的变化［J］.中华小儿外科杂志，2007，28（10）：545-549.

［4］Li C, Wang W, Knepper MA, Nielsen S, Fr-kiaer J. Downregulation of renal aquaporins in response to unilateral ureteral obstruction［J］. Am J Physiol Renal Physiol, 2003, 284(5): F1066-F1069.

［5］Li C, Wang W, Kwon TH, Isikay L, Wen JG, Marples D, et al. Downregulation of AQP1, -2, and-3 after ureteral obstruction is associated with a long-term urine-concentrating defect［J］. Am J Physiol Renal Physiol, 2001, 281(1): F163-F171.

［6］李真珍，文建国，张红，李文才，王焱，杨珂.轻度肾积水儿童肾脏水通道蛋白2、3和4的表达［J］.实用儿科临床杂志，2007，22（22）：1751-1752.

［7］文建国，王炎，李真珍，张国贤，王庆伟，杨黎，等.先天性肾积水肾脏及其尿液中水通道蛋白2表达的相关性研究［J］.中华小儿外科杂志，2009，30（2）：86-90.

［8］文建国，李真珍，范应中，张谦，张红，张国贤，等.先天性积水肾脏组织中水通道蛋白1-4 mRNA表达与肾实质厚度变化的相关性研究［J］.中华泌尿外科杂志，2009，30（4）：1417.

［9］Bobrik II, Dugan IV. The anatomy of human kidneys in ultrasonic study［J］. Vrach Delo, 1991,(5): 73-76.

［10］McDill BW, Li SZ, Kovach PA, Ding L, Chen F. Congenital progressive hydronephrosis (cph) is caused by an S256L mutation in aquaporin-2 that affects its phosphorylation and apical membrane accumulation［J］. Proc Natl Acad Sci U S A, 2006, 103(18): 6952-6957.

［11］林善锬.肾素、血管紧张素（RA）系统与临床［J］.中华肾脏病杂志，1989，5（4）：228.

［12］Thornhill BA, Burt LE, Chen C, Forbes MS, Chevalier RL. Variable chronic partial ureteral obstruction in the neonatal rat: a new model of ureteropelvic junction obstruction［J］. Kidney Int, 2005, 67(1): 4252.

［13］Huang WY, Peters CA, Zurakowski D, Borer JG, Diamond DA, Bauer SB, et al. Renal biopsy in congenital ureteropelvic junction obstruction: evidence for parenchymal maldevelopment［J］. Kidney Int, 2006, 69(1): 137-143.

［14］肖永志，周启昌，章鸣，彭清海，范平，云正芬，等.产前超声诊断胎儿泌尿系统发育不良的临床价值［J］.中国当代儿科杂志，2009，7（11）：593-595.

［15］文建国，周玉清，文兰田.B超测定正常儿童肾脏大小和形态［J］.河南医学研究，1995，4（2）：168-171.

［16］Perrome RD. Means of clinical evaluation of renal disease progression［J］. Kidney Int Suppl, 1992, 36: S26-S32.

［17］郑向红，宋厂义，王社教，刘清娥.肾动态显像在小儿先天性肾积水中的临床应用［J］.中国临床医学影像杂志，2000，11（6）：418-420.

［18］姜立新，郭宗远，吴荣德，于启海，刘占峰. 99mTc-DTPA肾动态显像评价新生儿肾积水肾功能［J］.中华核医学杂志，2004，24（2）：110-112.

［19］Shi Y, Li C, Thomsen K, J-rgensen TM, Knepper MA, Nielsen S, et al. Neonatal ureteral obstruction alters expression of renal sodium transporters and aquaporin water channels［J］. Kidney Int, 2004, 66(1): 203-215.

［20］Zhang PL, Peters CA, Rosen S. Ureteropelvic junction obstruction: morphological and clinical studies［J］. Pediatr Nephrol, 2000, 14(8-9): 820-826.