Expression of erythropoietin and its receptor in the brain of newborn rats suffering from fetal distress

ZHANG Zhi-Min; TIAN Zhao-Fang; LI Yu-Hong; ZHAO Sai

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Chinese Journal of Contemporary Pediatrics ›› 2011, Vol. 13 ›› Issue (11) : 912-916.

EXPERIMENTAL RESEARCH

Expression of erythropoietin and its receptor in the brain of newborn rats suffering from fetal distress

ZHANG Zhi-Min, TIAN Zhao-Fang, LI Yu-Hong, ZHAO Sai

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Abstract

OBJECTIVE: To study the expression of erythropoietin (EPO) and its receptor (EPOR) in the brain of newborn rats suffering fetal distress. METHODS: A model of fetal distress was prepared by ligating bilateral uterine arteries of the rats with full-term pregnancy for 10 minutes before cesarean sections. The expression levels of EPO and EPOR in the brain of newborn rats were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot at 0, 2, 6, 12, 24, 48, 72 hrs and 7 days after birth. Serum EPO levels were measured using ELISA simultaneously. The newborn rats born by cesarean sections which were not subjected to uterine artery ligation were used as the control group. RESULTS: The expression of EPO protein and mRNA in brain tissues in the fetal distress group increased significantly compared with the control group 2, 6 and 12 hrs after birth (P<0.05). The expression of EPOR protein and mRNA in brain tissues in the fetal distress group increased significantly compared with the control group 2, 6, 12, 24 and 48 hrs, and 3 days after birth (P<0.05). Serum EPO levels in the fetal distress group were significantly higher than in the control group 2 hrs after birth. CONCLUSIONS: The EPO and EPOR levels in the brain increase quickly after birth in newborn rats suffering from fetal distress. The EPOR is high expressed for a longer time than EPO. This can provide a basis for the treatment of neonatal brain damage induced by fetal distress by exogenous EPO.

Key words

Erythropoietin / Erythropoietin receptor / Brain / Fetal distress / Newborn rats

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ZHANG Zhi-Min, TIAN Zhao-Fang, LI Yu-Hong, ZHAO Sai. Expression of erythropoietin and its receptor in the brain of newborn rats suffering from fetal distress[J]. Chinese Journal of Contemporary Pediatrics. 2011, 13(11): 912-916

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