Th1/Th2 immune response in bronchoalveolar lavage fluid in children with severe Mycoplasma pneumoniae pneumonia

KANG  Yan-Meng; DING  Ming-Jie; HAN  Yu-Ling; WANG  Shi-Fu; MA  Xiang; LI  Hua

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Chinese Journal of Contemporary Pediatrics ›› 2011, Vol. 13 ›› Issue (3) : 188-190.

CLINICAL RESEARCH

Th1/Th2 immune response in bronchoalveolar lavage fluid in children with severe Mycoplasma pneumoniae pneumonia

KANG Yan-Meng, DING Ming-Jie, HAN Yu-Ling, WANG Shi-Fu, MA Xiang, LI Hua

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Abstract

OBJECTIVE: To study the status of Th1/Th2 immune response and the value of the detection of cytokines in bronchoalveolar lavage fluids (BALF) by examining the levels of IFN-γ and IL-4 in BALF and serum in children with severe Mycoplasma pneumonia (MPP). METHODS: The levels of IFN-γ and IL-4 in BALF and serum were measured using ELISA in 25 children with severe MPP, 25 children with mild MPP and 25 children with foreign body in bronchus. RESULTS: The levels of IL-4 and IFN-γ and the IL-4/IFN-γ ratio in BALF in children with severe MPP were significantly higher than those in children with foreign body in bronchus (P<0.01 or <0.05). The serum levels of IL-4 and the IL-4/IFN-γ ratio in children with severe MPP were significantly higher than those in children with foreign body in bronchus (P<0.01) or with mild MPP (P<0.05). The levels of IL-4 and the IL-4/IFN-γ ratio in BALF were significantly higher than in serum (P<0.05). CONCLUSIONS: The data suggest that the imbalance of Th1/Th2 exists in children with severe MPP and it seems to represent a predominant Th2-like cytokine response. The detection of cytokines in BALF appears to be more sensitive than in serum and may be of value in the diagnosis and therapy of MPP.

Key words

Severe Mycoplasma pneumoniae pneumonia / Bronchoalveolar lavage fluid / Th1/Th2 / Child

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KANG Yan-Meng, DING Ming-Jie, HAN Yu-Ling, WANG Shi-Fu, MA Xiang, LI Hua. Th1/Th2 immune response in bronchoalveolar lavage fluid in children with severe Mycoplasma pneumoniae pneumonia[J]. Chinese Journal of Contemporary Pediatrics. 2011, 13(3): 188-190

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