Methylation of insulin-like growth factor binding protein 3 gene in neonates with intrauterine growth restriction

SU Ai-Ling; JIANG Li; GE Qin-Yu

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Chinese Journal of Contemporary Pediatrics ›› 2011, Vol. 13 ›› Issue (9) : 700-703.

CLINICAL RESEARCH

Methylation of insulin-like growth factor binding protein 3 gene in neonates with intrauterine growth restriction

SU Ai-Ling, JIANG Li, GE Qin-Yu

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Abstract

OBJECTIVE: To study the role of promoter methylation of insulin-like growth factor binding protein 3 (IGFBP3) in intrauterine growth restriction (IUGR). METHODS: Fifty neonates with IUGR and 30 healthy neonates were enrolled. The promoter methylation status of IGFBP3 in peripheral blood was evaluated by methylation-specific PCR (MSP) and high resolution melting (HRM) techniques. RESULTS: The complete methylation rate, partial methylation rate and non-methylation rate of IGFBP3 promoter in the IUGR group was 4% (2/50), 40% (20/50) and 56% (28/50), respectively. The partial methylation rate and non-methylation rate of IGFBP3 promoter in the control group were 13% (4/30) and 87% (26/30), respectively. There were significant differences in the promoter methylation rate of IGFBP3 between the two groups (P<0.01). CONCLUSIONS: The promoter methylation of IGFBP3 gene is associated with the pathogenesis of IUGR.

Key words

Intrauterine growth restriction / Insulin-like growth factor binding protein 3 / Methylation / Neonate

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SU Ai-Ling, JIANG Li, GE Qin-Yu. Methylation of insulin-like growth factor binding protein 3 gene in neonates with intrauterine growth restriction[J]. Chinese Journal of Contemporary Pediatrics. 2011, 13(9): 700-703

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