Changes to surfactant proteins in the bronchoalveolar lavage fluid and serum of children with Mycoplasma pneumoniae pneumonia

SHU Lin-Hua; SHANG Yun-Xiao; CAI Xu-Xu; ZHANG Han; LI Miao; ZHOU Qian-Lan; ZONG Zhi-Hong; MENG Xiao-Na; LI Xiang; LIU Fen

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Chinese Journal of Contemporary Pediatrics ›› 2012, Vol. 14 ›› Issue (12) : 928-932.

Changes to surfactant proteins in the bronchoalveolar lavage fluid and serum of children with Mycoplasma pneumoniae pneumonia

SHU Lin-Hua, SHANG Yun-Xiao, CAI Xu-Xu, ZHANG Han, LI Miao, ZHOU Qian-Lan, ZONG Zhi-Hong, MENG Xiao-Na, LI Xiang, LIU Fen

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Abstract

OBJECTIVE: To study the changes to surfactant proteins in the serum and bronchoalveolar lavage fluids (BALF) of children with Mycoplasma pneumoniae pneumonia (MPP) and their significance. METHODS: Self-control method was used in the study. Forty-seven MPP children were divided into single lung infected (n=32) and bilateral lung infected groups (n=15) according to lung CT results. Surfactant proteins SP-A, B, C and D were measured using ELISA in the serum and BALF in the two groups. The correlations between SP-A, B, C and D content in the serum and BALF were evaluated by Spearman correlation analysis. RESULTS: SP-A, B, C and D content in BALF from the majorly infected or infected lung were significantly higher than from the opposite lung and serum (P<0.01). SP-A, B and C content in serum was significantly lower than in BALF from the non-infected lung in the single-side infected lung group (P<0.01 or 0.05), but there was no significant difference between serum SP-D content and BALF SP-D content from the non-infected lung. There were no significant differences in SP-A, B, C and D content in serum and BALF from the minorly infected lung in the bilateral lung infected group. Serum SP-D content was positively correlated with BALF SP-D content from the majorly infected lung in the bilateral lung infected group (P<0.01). CONCLUSIONS: Serum SP-D content may serve as a biomarker for evaluating the severity of pulmonary infection in children with community-acquired pneumonia.

Key words

Community-acquired pneumonia / Bronchoalveolar lavage fliud / Pulmonary surfactant protein / Child

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SHU Lin-Hua, SHANG Yun-Xiao, CAI Xu-Xu, ZHANG Han, LI Miao, ZHOU Qian-Lan, ZONG Zhi-Hong, MENG Xiao-Na, LI Xiang, LIU Fen. Changes to surfactant proteins in the bronchoalveolar lavage fluid and serum of children with Mycoplasma pneumoniae pneumonia[J]. Chinese Journal of Contemporary Pediatrics. 2012, 14(12): 928-932

References

［1］Rudan I, Chan KY, Zhang JS, Theodoratou E, Feng XL, Salomon JA, et al. Causes of deaths in children younger then 5 years in China in 2008［J］. Lancet, 2010, 375(9720): 1083-1089.

［2］Liu L, Johnson HL, Cousens S, Perin J, Scott S, Lawn JE, et al. Global, regional, and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000［J］. Lancet, 2012, 379（9832）: 2151-2161.

［3］Whitsett JA, Wert SE, Weaver TE. Alveolar surfactant homeostasis and the pathogenesis of pulmonary disease［J］. Annu Rev Med, 2010, 61: 105-119.

［4］Kuroki Y, Takahashi M, Nishitani C. Pulmonary collectins in innate immunity of the lung［J］. Cell Microbiol, 2007, 9(8): 1871-1879.

［5］Whitsett JA. Surfactant proteins in innate host defense of the lung ［J］. Biol Neonate, 2005, 88(3): 175-180.

［6］舒林华，吴秀清，魏克伦，舒林宏，薛辛东，吴红敏，等. 肺表面活性物质蛋白-D在脂多糖诱导的急性肺损伤幼鼠中的时序变化［J］［英文］. 中国当代儿科杂志，2005，7（6）：483-488.

［7］舒林华，魏克伦，刘春峰，韩晓华，尚云晓，蔡栩栩，等. 对LPS诱导的急性肺损伤幼鼠肺组织SP-A变化的研究［J］. 中国当代儿科杂志，2008，10（2）：203-206.

［8］Todd DA, Marsh MJ, George A, Henderson NG, Barr H, Sebastian S, et al. Surfactant phospholipids, surfactant proteins, and inflammatory markers during acute lung injury in children［J］. Pediatr Crit Care Med, 2010, 11(1): 82-91.

［9］Leth-Larsen R, Nordenbaek C, Tornoe I, Moeller V, Schlosser A, Koch C, et al. Surfactant protein D (SP-D) serum levels in patients with community-acquired pneumonia small star, filled［J］. Clin Immunol, 2003, 108(1): 29-37.

［10］Kishore U, Bernal AL, Kamran MF, Saxena S, Singh M, Sarma PU, et al. Surfactant proteins SP-A and SP-D in human health and disease［J］. Arch Immunol Ther Exp (Warsz), 2005, 53(5): 399-417.

［11］Almlen A, Stichtenoth G, Linderholm B, Haegerstrand-Bjorkman M, Robertson B, Johansson J, et al. Surfactant proteins B and C are both necessary for alveolar stability at end expiration in premature rabbits with respiratory distress syndrome［J］. J Appl Physiol, 2008, 104(4): 1101-1108.

［12］Chroneos ZC, Sever-Chroneos Z, Shepherd VL. Pulmonary surfactant: an immunological perspective［J］. Cell Physiol Biochem, 2010, 25(1): 13-26.

［13］中华医学会儿科分会呼吸学组，中华儿科杂志编辑委员会. 儿童社区获得性肺炎管理指南（试行）（上）［J］. 中华儿科杂志，2007,45（2）：83-90.

［14］中华医学会儿科分会呼吸学组，中华儿科杂志编辑委员会. 儿童社区获得性肺炎管理指南（试行）（下）［J］. 中华儿科杂志，2007,45（3）：223-230.

［15］中华医学会儿科分会呼吸学组儿科支气管镜协作组. 儿科支气管镜术指南（2009年版）［J］. 中华儿科杂志，2009，47（10）：740-744.

［16］Daimon T, Tajima S, Oshikawa K, Bando M, Ohno S, Sugiyama Y. KL-6 and surfactant proteins a and D in serum and bronchoalveolar lavage fluid in patients with acute eosinophilic pneumonia［J］. Inter Med, 2005, 44(8): 811-817.

［17］Ariki S, Nishitani C, Kuroki Y. Diverse functions of pulmonary collectins in host defense of the lung［J］. J Biomed Biotechnol, 2012, 2012: 532071.

［18］舒林华，尚云晓，张福会，张晗，马晓雪，杨男，等. 肺炎链球菌对小鼠及儿童肺泡Ⅱ型上皮细胞超微结构的影响［J］. 中国当代儿科杂志，2011，13（4）：336-339.

［19］韩丽英，舒林华，尚云晓，韩晓华，蔡栩栩，张晗. 先天性多发性支气管源性肺囊肿患儿1例［J］. 山东医药，2011,51（24）：44.

［20］Rottem S. Interaction of Mycoplasmas with host cells［J］. physiol Rev, 2003, 83(2): 417-432.

［21］Ledford JG, Goto H, Potts EN, Degan S, Chu HW, Voelker DR, et al. SP-A preserves airway homeostasis during Mycoplasma pneumoniae infection in mice［J］. J Immunol, 2009, 182(2): 7818-7827.

［22］Piboonpocanun S, Chiba H, Mitsuzawa H, Martin W, Murphy RC, Harbeck RJ, et al. Surfactant protein A binds Mycoplasma pneumoniae with high affinity and attenuates its growth by recognition of disaturated phosphatidylglycerols［J］. J Biol Chem, 2005, 280(1): 9-17.

［23］Tafel O, Latzin P, Paul K, Winter T, Woischnik M, Griese M. Surfaetant proteins SP-B and SP-C and their precursors in bronchoalveolar lavages from children with acute and chronic inflammatory airway disease［J］. BMC Pulm Med, 2008, 8: 6.

［24］舒林华，张晗，尚云晓，宗志红，相云，刘立云，等.2009年流行性甲型H1N1流感重症肺炎患儿血清肺表面活性蛋白A、B、C、D的变化［J］. 山东医药，2011，51（48）：16-18.