Bufalin inhibits PDGF-BB-induced mesangial cell proliferation via mediating gap junctional intercellular communication

HAN Ying; ZHANG Ai-Qing; ZHANG Jun; GONG Jing; LI Shan-Wen; GAN Wei-Hua

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Chinese Journal of Contemporary Pediatrics ›› 2012, Vol. 14 ›› Issue (12) : 982-987.

EXPERIMENTAL RESEARCH

Bufalin inhibits PDGF-BB-induced mesangial cell proliferation via mediating gap junctional intercellular communication

HAN Ying, ZHANG Ai-Qing, ZHANG Jun, GONG Jing, LI Shan-Wen, GAN Wei-Hua

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Abstract

OBJECTIVE: To study the role and possible mechanisms of gap junctional intercellular communication (GJIC) involved in mesangial cell (MC) proliferation which could be inhibited by bufalin. METHODS: Rat mesangial cells were cultured in vitro. The effect of bufalin on platelet-derived growth factor-BB (PDGF-BB)-induced MC proliferation was evaluated by MTT assay. The function of GJIC was detected by Lucifer Yellow scrape loading and dye transfer (SLDT). mRNA levels of Cx43, Cx45 and Cx40 were measured by RT-PCR. Intracellular calcium concentrations ([Ca2+]i) were examined in laser scanning confocal microscopy after loading by Fura-3/AM. RESULTS: MTT indicated that bufalin could inhibited PDGF-BB-induced MC proliferation (P0.05) in gene level and also increased [Ca2+]i. However, bufalin treatment enhanced GJIC function, decreased Cx45 mRNA and Cx40 mRNA expression (P<0.01), and reduced [Ca2+]i (P<0.01). CONCLUSIONS: Bufalin inhibits PDGF-BB-induced MC proliferation, and its possible mechanisms may be related to regulation of Cx45 and Cx40 expression in the gene level, reducion of [Ca2+]i and enhancment of GJIC function.

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Gap Junction / Bufalin / Cell communication / PDGF-BB / Mesangial cell

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HAN Ying, ZHANG Ai-Qing, ZHANG Jun, GONG Jing, LI Shan-Wen, GAN Wei-Hua. Bufalin inhibits PDGF-BB-induced mesangial cell proliferation via mediating gap junctional intercellular communication[J]. Chinese Journal of Contemporary Pediatrics. 2012, 14(12): 982-987

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