Roles of PKCα on the biological functions of T cells

YANG Li-Fen; KONG Hui-Min; ZHANG Xiao-Qing; YIN Fei

doi:10.7499/j.issn.1008-8830.2015.12.019

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Chinese Journal of Contemporary Pediatrics ›› 2015, Vol. 17 ›› Issue (12) : 1354-1359. DOI: 10.7499/j.issn.1008-8830.2015.12.019

EXPERIMENTAL RESEARCH

Roles of PKCα on the biological functions of T cells

YANG Li-Fen, KONG Hui-Min, ZHANG Xiao-Qing, YIN Fei

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Abstract

Objective To study the roles of PKCα on the proliferation, apoptosis, differentiation, cytokine production and inducible regulatory T cell (iTreg) induction of T cells. Methods T cells from WT (PKCα+/+) or PKCα knockout (PKCα-/-) mice were isolated and cultured in vitro. T cell proliferation and apoptosis were determined using 3H thymidine incorporation and CSFE/Annexin V staining. Cytokines production (IL-2, IL-4, IFN-γ and IL-17) was detected using ELISA. CD4+T cells were isolated and cultured in vitro via Th17 or iTreg biased condition. Flow cytometry was used to detect the cell differentiation. Results The production of IL-2 upon TCR stimulation increased, while the contents of IL-4 and IL-17 decreased in the PKCα-/- group compared with the PKCα+/+ group. The differentiation rate of Th17 cells decreased, while the iTreg production increased in the PKCα-/- group compared with the PKCα+/+ group. Conclusions PKC-α is proinflammatory.

Key words

PKCα / T cell / Th17 cell / Inducible regulatory T cell

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YANG Li-Fen, KONG Hui-Min, ZHANG Xiao-Qing, YIN Fei. Roles of PKCα on the biological functions of T cells[J]. Chinese Journal of Contemporary Pediatrics. 2015, 17(12): 1354-1359 https://doi.org/10.7499/j.issn.1008-8830.2015.12.019

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