Relationship between MTHFR gene polymorphism and susceptibility to bronchial asthma and glucocorticoid efficacy in children

LI Min; TANG Yu; ZHAO Er-Yao; CHEN Chao-Hui; DONG Li-Li

doi:10.7499/j.issn.1008-8830.2105035

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Chinese Journal of Contemporary Pediatrics ›› 2021, Vol. 23 ›› Issue (8) : 802-808. DOI: 10.7499/j.issn.1008-8830.2105035

CLINICAL RESEARCH

Relationship between MTHFR gene polymorphism and susceptibility to bronchial asthma and glucocorticoid efficacy in children

LI Min, TANG Yu, ZHAO Er-Yao, CHEN Chao-Hui, DONG Li-Li

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Abstract

Objective To study the association of methylenetetrahydrofolate reductase (MTHFR) gene polymorphism with susceptibility to bronchial asthma and glucocorticoid (GC) efficacy in children. Methods A total of 173 children with bronchial asthma who were hospitalized between June 2018 and December 2020 were selected as the observation group. The children received aerosol inhalation of GC for three consecutive months. A total of 178 healthy children who underwent physical examination during the same period were selected as the control group. PCR was used to detect the genotypes of the MTHFR C677T for the two groups. The differences in genotype distribution between the two groups were analyzed. Children with different genotypes in the observation group were compared in terms of immunoglobulin E (IgE), interleukin-8 (IL-8), leukotriene B4 (LTB4), lung function, and clinical outcome before and after treatment. Results TT genotype and T allele were significantly more frequent in the observation group than in the control group (P<0.001). TT/CT genotypes and T allele were independent risk factors for bronchial asthma (OR=6.615 and 7.055 respectively; P<0.001). After GC treatment, the children with CC, CT or TT genotypes experienced significantly decreased levels of IgE, IL-8, and LTB4 and significantly increased forced expiratory volume in 1 second (FEV1), forced vital capacity (FVC), and FEV1/FVC ratio (P<0.001). The children with TT genotype showed significantly lower levels of IL-8 and LTB4 than those with CC genotype, a significantly lower level of LTB4 than those with CT genotype, significantly higher FVC than those with CT genotype, and a significantly higher FEV1/FVC ratio than those with CC genotype (P<0.05). The children with TT genotype had better GC efficacy compared with those with CC genotype (P<0.05). TT genotype was an independent factor for good GC efficacy (OR=2.111, P=0.018). Conclusions MTHFR gene polymorphism is associated with asthma susceptibility and GC efficacy in children. Children carrying TT/CT genotypes have a higher risk of developing asthma, and those with TT genotype are more sensitive to GC treatment.

Key words

Bronchial asthma / Methylenetetrahydrofolate reductase / Gene polymorphism / Glucocorticoid / Child

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LI Min, TANG Yu, ZHAO Er-Yao, CHEN Chao-Hui, DONG Li-Li. Relationship between MTHFR gene polymorphism and susceptibility to bronchial asthma and glucocorticoid efficacy in children[J]. Chinese Journal of Contemporary Pediatrics. 2021, 23(8): 802-808 https://doi.org/10.7499/j.issn.1008-8830.2105035

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