Diagnostic efficacy of serum 14-3-3β protein combined with fractional exhaled nitric oxide and conventional ventilatory lung function parameters for bronchial asthma in children

LI Shu-Fang; GUO Guang-En; YANG Yue-Qin; XIONG Xiao-Man; ZHENG Shi-Wei; XIE Xue-Li; ZHANG Yan-Li

doi:10.7499/j.issn.1008-8830.2401058

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Chinese Journal of Contemporary Pediatrics ›› 2024, Vol. 26 ›› Issue (7) : 723-729. DOI: 10.7499/j.issn.1008-8830.2401058

CLINICAL RESEARCH

Diagnostic efficacy of serum 14-3-3β protein combined with fractional exhaled nitric oxide and conventional ventilatory lung function parameters for bronchial asthma in children

LI Shu-Fang, GUO Guang-En, YANG Yue-Qin, XIONG Xiao-Man, ZHENG Shi-Wei, XIE Xue-Li, ZHANG Yan-Li

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Abstract

Objective To explore the diagnostic efficacy of serum 14-3-3β protein combined with fractional exhaled nitric oxide (FeNO) and conventional ventilatory lung function parameters in diagnosing bronchial asthma (referred to as "asthma") in children. Methods A prospective study included 136 children initially diagnosed with asthma during an acute episode as the asthma group, and 85 healthy children undergoing routine health checks as the control group. The study compared the differences in serum 14-3-3β protein concentrations between the two groups, analyzed the correlation of serum 14-3-3β protein with clinical indices, and evaluated the diagnostic efficacy of combining 14-3-3β protein, FeNO, and conventional ventilatory lung function parameters for asthma in children. Results The concentration of serum 14-3-3β protein was higher in the asthma group than in the control group (P<0.001). Serum 14-3-3β protein showed a positive correlation with the percentage of neutrophils and total serum immunoglobulin E, and a negative correlation with conventional ventilatory lung function parameters (P<0.05). Cross-validation of combined indices showed that the combination of 14-3-3β protein, FeNO, and the percentage of predicted value of forced expiratory flow at 75% of lung volume had an area under the curve of 0.948 for predicting asthma, with a sensitivity and specificity of 88.9% and 93.7%, respectively, demonstrating good diagnostic efficacy (P<0.001). The model had the best extrapolation. Conclusions The combination of serum 14-3-3β protein, FeNO, and the percentage of predicted value of forced expiratory flow at 75% of lung volume can significantly improve the diagnostic efficacy for asthma in children. Citation:Chinese Journal of Contemporary Pediatrics, 2024, 26(7): 723-729

Key words

Bronchial asthma / 14-3-3β protein / Combined diagnosis / Lung ventilatory function / Exhaled nitric oxide / Child

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LI Shu-Fang, GUO Guang-En, YANG Yue-Qin, XIONG Xiao-Man, ZHENG Shi-Wei, XIE Xue-Li, ZHANG Yan-Li. Diagnostic efficacy of serum 14-3-3β protein combined with fractional exhaled nitric oxide and conventional ventilatory lung function parameters for bronchial asthma in children[J]. Chinese Journal of Contemporary Pediatrics. 2024, 26(7): 723-729 https://doi.org/10.7499/j.issn.1008-8830.2401058

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