Abstract：ObjectiveTo study the role of serum from asphyxiated neonates in the inducement of human renal proximal tubular epithelial cells (HK2) adhesion to neutrophils and possible mechanisms.MethodsHK2 cells were cultured randomly with 20% serum from neonates (1, 3, and 7 days after asphyxia), pyrrolidine dithiocarbamate (PDTC) or placebo. The activity of myeloperoxidase (MPO), an indicator of adhesion ability of HK2 cells to neutrophils in suspensions, was detected by the biochemistry assay. Intercellular adhesion molecule1 (ICAM1) and nuclear factorκB (NFκB) of HK2 cells were examined with the immunohistochemical staining.ResultsThe expression of MPO in the postasphyxial 1day serum treatment group were significantly higher than that in the PDTC treatment and the control groups as well as the postasphyxial 3 and 7day serum treatment groups (P<0.01). The expression of ICAM1 and NFκB in the postasphyxial 1day serum treatment group was also significantly higher than that in the other groups (P<0.01).ConclusionsSerum from asphyxiated neonates can induce HK2 cell adhesion to neutrophils, possibly through activating NFκB and increasing the synthesis and expression of ICAM1 on the surface of renal tubular epithelial cells.
FU Hui,DONG Wen-Bin,LONG Han-An et al. Role of serum from asphyxiated neonates in the inducement of human renal tubular cell adhesion to neutrophils[J]. CJCP, 2008, 10(5): 633-636.
