Abstract：Objective To explore the abnormal expression of plasma proteins by analysis of proteomic expression profile in children with infectious mononucleosis (IM). Methods Two dimensional gel electrophoresis (2-DE) followed by the mass spectrometry was used to examine important protein spots with different expression levels between children with IM and normal controls. Results Seven differential proteins were obtained: hemopexin, vitamin D binding protein, fetuin A, C-reactive protein, apolipoprotein A, haptoglobin and transthyretin. Compared with the control group, haptoglobin showed a higher expression level in children with IM, and the expression levels of the other proteins were obviously down-regulated. Conclusions The expression changes of differential proteins identified in this study are all related with the liver acute injury, suggesting that children with IM are associated with acute liver injury. Further studies on the characteristics of above proteins will contribute to the diagnosis and treatment of pediatric IM.
Rolla S, Ingoglia G, Bardina V, et al. Acute-phase protein hemopexin is a negative regulator of Th17 response and experimental autoimmune encephalomyelitis development[J]. J Immunol, 2013, 191(11): 5451-5459.
[8]
Larsen R, Gozzelino R, Jeney V, et al. A central role for free heme in the pathogenesis of severe sepsis[J]. Sci Transl Med, 2010, 2(51): 51-71.
[9]
Lin T, Sammy F, Yang H, et al. Identification of hemopexin as an anti-inflammatory factor that inhibits synergy of hemoglobin with HMGB1 in sterile and infectious inflammation[J]. J Immunol, 2012, 189(4): 2017-2022.
[10]
Speeckaert MM, Speeckaert R, van Geel N, et al. Vitamin D binding protein: a multifunctional protein of clinical importance[J]. Adv Clin Chem, 2014, 63: 1-57.
[11]
Dziegielewska KM, Brown WM, Gould CC, et al. Fetuin: an acute phase protein in cattle[J]. J Comp Physiol B, 1992, 162(2): 168- 171.
[12]
Kang WC, Ahn TH, Moon CI, et al. Impact of arterial remodeling on high sensitive C-reactive protein after a DES implantation[J]. Ant J Cardiol, 2010, 145(2): 325-326.
[13]
van de Veerdonk FL, Wever PC, Hermans MH, et al. IL-18 serum concentration is markedly elevated in acute EBV infection and can serve as a marker for disease severity[J]. J Infect Dis, 2012, 206(2): 197-201.
Kaysen GA. Malnutrition and the acute-phase reaction in dialysis patients-how to measure and how to distinguish[J]. Nephrol Dial Transplant, 2000, 15(10): 1521-1524.
[16]
Sarvari J, Mojtahedi Z, Kuramitsu Y, et al. Differential expression of haptoglobin isoforms in chronic active hepatitis, cirrhosis and HCC related to HBV infection[J]. Oncol Lett, 2011, 2(5): 871-877.
[17]
Borlak J, Chatterji B, Londhe KB, et al. Serum acute phase reactants hallmark healthy individuals at risk for acetaminopheninduced liver injury[J]. Genome Med, 2013, 5(9): 86-99.
[18]
Yao Y, Duan HM, Yan HM, et a1. Clinically controlled study on children,S infectious mononucleosis treated by Chinese medicine[J]. Chin J Integr Med, 2009, 15(5): 347-352.
