Abstract：Objective To study the expression of Foxp3 and NFAT1 protein in peripheral blood (PB) in children with aplastic anemia (AA) and their roles in the pathogenesis of AA. Methods The expression levels of Foxp3 and NFAT1 protein of mononuclear cells in PB were measured by Western blot in 68 children with AA before and after treatment and in 60 normal children (control group). The correlation between Foxp3 and NFAT1 protein expression and the correlation of the Foxp3 and NFAT1 protein expression with blood Hb, WBC and platelet levels were analyzed. Results The expression levels of Foxp3 and NFAT1 protein in PB in the acute phase in the AA group were significantly lower than in the control group (PPr=0.812, Pr=0.537, 0.579, 0.655 respectively; PConclusions The Foxp3 and NFAT1 protein levels in PB are reduced in children with AA, suggesting that they are involved in the pathogenesis of AA. The measurement of Foxp3 and NFAT1 protein levels may be useful in the severity evaluation of AA.
Solomou EE, Rezvani K, Mielke S, et al. Deficient CD4+CD25+FOXP3+ T regulatory cells in acquired aplastic anemia[J]. Blood, 2007, 110(5): 1603-1606.
[4]
Chen J, Ellison FM, Eckhaus MA, et al. Minor antigen H60- mediated aplastic anemia is ameliorated by immunosuppression and the infusion of regulatory T cells[J]. J Immunol, 2007, 178(7): 4159-4168.
[5]
Park JH, Ko JS, Shin Y, et al. Intranuclear interactomic inhibition of Foxp3 suppresses functions of Treg cells[J]. Biochem Biophys Res Commun, 2014, 451(1): 1-7.
[6]
Fontenot JD, Rudensky AY. A well adapted regulatory contriveance: regulatory T cell development and the forkhead family transcription factor Foxp3[J]. NAT Immunol, 2005, 6(4): 331-337.
[7]
Sakaguchi S. Regulatory T cells: history and perspective[J]. Methods Mol Biol, 2011, 707(10): 3-17.
[8]
Ghosh S, Koralov SB, Stevanovic I, et al. Hyperactivation of nuclear factor of activated T cells 1 (NFAT1) in T cells attenuates severity of murine autoimmune encephalomyelitis[J]. Proc Natl Acad Sci USA, 2010, 107(34): 15169-15174.
[9]
Bettelli E, Dastrange M, Oukka M. Foxp3 interacts with nuclear factor of activated T cells and NF-κB to repress cytokine gene expression and effector functions of T helper ceells[J]. Proc Natl Acad Sci USA, 2005, 102(14): 5138-5143.
[10]
Li Q, Shakya A, Guo X, et al. Constitutive nuclear localization of NFAT in Foxp3+ regulatory T cells independent of calcineurin activity[J]. J Immunol, 2012, 188(9): 4268-4277.
[11]
Vaeth M, Schliesser U, Muller G, et al. Dependence on nuclear factor of activated T-cells (NFAT) levels discriminates conventional T cells from Foxp3+ regulatory T cells[J]. Proc Natl Acad Sci USA, 2012, 109(40): 16258-16263.
