Abstract：Objective To investigate the role of long non-coding RNA (lncRNA) BC088414 in hypoxic-ischemic injury of neural cells. Methods Rat adrenal pheochromocytoma (PC12) cells were divided into four groups:normoxic, oxygen glucose deprivation (OGD), siRNA-normoxic (siRNA group) and siRNA-OGD (n=3 each). Cells were incubated in glucose-free and serum-free DMEM medium under the conditions of 37℃ and 1% O2+99% N2/CO2 for 6 hours to establish an in vitro hypoxic-ischemic model. Quantitative real-time PCR was used to measure mRNA expression of lncRNA BC088414, β2-adrenoceptor (Adrb2), and caspase-6(CASP6). siRNAs were used to inhibit BC088414 expression in PC12 cells. The TUNEL method was used to measure cell apoptosis. Results The OGD group had a significantly higher cell apoptotic index than the normoxic group (PPPPPConclusions LncRNA BC088414 may promote apoptosis through Adrb2 and CASP6 and aggravate neural cell injury induced by hypoxia-ischemia.
ZHAO Feng-Yan,TANG Jun,ZHANG Li et al. Role of long non-coding RNA BC088414 in hypoxic-ischemic injury of neural cells[J]. CJCP, 2015, 17(12): 1348-1353.
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