含jumonji结构域的蛋白2B和缺氧诱导因子1α在儿童非霍奇金淋巴瘤组织中的表达及意义

doi:10.7499/j.issn.1008-8830.2305025

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摘要目的探讨含jumonji结构域的蛋白2B（jumonji domain-containing protein 2B, JMJD2B）和缺氧诱导因子1α（hypoxia inducible factor-1α, HIF-lα）在儿童非霍奇金淋巴瘤（non-Hodgkin's lymphoma, NHL）组织中的表达和意义。方法应用免疫组化检测46例NHL儿童（观察组）和24例反应性增生儿童（对照组）淋巴结组织标本中JMJD2B和HIF-1α的表达，分析JMJD2B和HIF-1α与NHL患儿临床病理特征和预后的关系，以及在NHL组织中JMJD2B和HIF-1α表达的相关性。结果观察组JMJD2B（87% vs 21%）和HIF-1α（83% vs 42%）表达阳性率均高于对照组（P<0.05）。JMJD2B和HIF-1α表达与NHL患儿血清乳酸脱氢酶水平和国际预后指数危险度相关（P<0.05）。JMJD2B与HIF-1α在NHL患儿组织中的表达呈正相关（r_s=0.333，P=0.024）。结论 JMJD2B和HIF-1α在儿童NHL组织中表达增高，二者在儿童NHL发生发展过程中可能起协同作用；JMJD2B可作为儿童NHL辅助诊断、评估病情、指导治疗、评估预后的新指标。

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	刁玉巧
	王俭
	朱秀丽
	陈健
	郑钰
	江莲
	刘月平
	戴若恒
	闫祎炜

关键词 ：非霍奇金淋巴瘤, 含jumonji结构域的蛋白2B, 缺氧诱导因子1α, 临床病理特征, 预后, 儿童

Abstract：Objective To investigate the expression and significance of jumonji domain-containing protein 2B (JMJD2B) and hypoxia-inducible factor-1α (HIF-1α) in non-Hodgkin's lymphoma (NHL) tissues in children. Methods Immunohistochemistry was used to detect the expression of JMJD2B and HIF-1α in lymph node tissue specimens from 46 children with NHL (observation group) and 24 children with reactive hyperplasia (control group). The relationship between JMJD2B and HIF-1α expression with clinicopathological characteristics and prognosis in children with NHL, as well as the correlation between JMJD2B and HIF-1α expression in NHL tissues, were analyzed. Results The positive expression rates of JMJD2B (87% vs 21%) and HIF-1α (83% vs 42%) in the observation group were higher than those in the control group (P<0.05). The expression of JMJD2B and HIF-1α was correlated with serum lactate dehydrogenase levels and the risk of international prognostic index in children with NHL (P<0.05). The expression of JMJD2B was positively correlated with the HIF-1α expression in children with NHL (rs=0.333, P=0.024). Conclusions JMJD2B and HIF-1α are upregulated in children with NHL, and they may play a synergistic role in the development of pediatric NHL. JMJD2B can serve as a novel indicator for auxiliary diagnosis, evaluation of the severity, treatment guidance, and prognosis assessment in pediatric NHL.

Key words： Non-Hodgkin's lymphoma Jumonji domain-containing protein 2B Hypoxia-inducible factor-1α Clinicopathological characteristic Prognosis Child

收稿日期: 2023-05-09

基金资助:河北省重点研发计划项目（20377794D）。

作者简介: 刁玉巧，女，硕士，主任医师。Email：dyqek@126.com；

引用本文:

刁玉巧,王俭,朱秀丽等. 含jumonji结构域的蛋白2B和缺氧诱导因子1α在儿童非霍奇金淋巴瘤组织中的表达及意义[J]. 中国当代儿科杂志, 2023, 25(11): 1150-1155.

DIAO Yu-Qiao,WANG Jian,ZHU Xiu-Li et al. Expression and significance of jumonji domain-containing protein 2B and hypoxia inducible factor-1α in non-Hodgkin lymphoma tissues in children[J]. CJCP, 2023, 25(11): 1150-1155.

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