Expression of interleukin-37, vascular endothelial growth factor A, and transforming growth factor-β1 and their correlation with T cells in children with primary immune thrombocytopenia
TONG Lin-Lin, WANG Li-Hua, FANG Fang, XU Bin, ZHENG Su-Hua
Department of Pediatrics, Handan Central Hospital, Handan, Hebei 056001, China
Abstract：Objective To investigate the expression of interleukin-37 (IL-37), vascular endothelial growth factor A (VEGFA), and transforming growth factor-β1 (TGF-β1) in children with primary immune thrombocytopenia (ITP) and their correlation with T cells. Methods A retrospective analysis was conducted on 45 children with ITP (ITP group) who were admitted to Handan Central Hospital from January 2020 to April 2022, and 30 healthy children who underwent physical examination during the same period were included as the healthy control group. The mRNA expression levels of IL-37, VEGFA, and TGF-β1 and the levels of regulatory T cells (Treg) and helper T cells 17 (Th17) were measured before and after treatment, and the correlation between the mRNA expression levels of IL-37, VEGFA, and TGF-β1 and the levels of Treg, Th17, and Treg/Th17 ratio were analyzed. Results Compared with the healthy control group, the ITP group had a significantly higher mRNA expression level of IL-37 and a significantly higher level of Th17 before and after treatment, as well as significantly lower mRNA expression levels of VEGFA and TGF-β1 and significantly lower levels of Treg and Treg/Th17 ratio (P<0.05). After treatment, the ITP group had significant reductions in the mRNA expression level of IL-37 and the level of Th17 and significant increases in the mRNA expression levels of VEGFA and TGF-β1 and the levels of Treg and Treg/Th17 ratio (P<0.05). Correlation analysis showed that in the ITP group, the mRNA expression levels of IL-37 and TGF-β1 were negatively correlated with the levels of Treg and Treg/Th17 ratio (P<0.05) and were positively correlated with the level of Th17 (P<0.05) before and after treatment; the mRNA expression level of VEGFA was positively correlated with the levels of Treg and Treg/Th17 ratio (P<0.05) and was negatively correlated with the Th17 level (P<0.05) before and after treatment. Conclusions Abnormal expression levels of IL-37, VEGFA, and TGF-β1 may be observed in children with ITP, which is significantly associated with the imbalance of Treg/Th17 ratio. It is speculated that the cytokines such as IL-37, VEGFA, and TGF-β1 may be involved in the development and progression of ITP or may become important potential targets for the treatment of children with ITP. Citation:Chinese Journal of Contemporary Pediatrics, 2023, 25(11): 1131-1136
TONG Lin-Lin,WANG Li-Hua,FANG Fang et al. Expression of interleukin-37, vascular endothelial growth factor A, and transforming growth factor-β1 and their correlation with T cells in children with primary immune thrombocytopenia[J]. CJCP, 2023, 25(11): 1131-1136.
Zhao Y, Ni X, Xu P, et al. Interleukin-37 reduces inflammation and impairs phagocytosis of platelets in immune thrombocytopenia (ITP)[J]. Cytokine, 2020, 125: 154853. PMID: 31557634. DOI: 10.1016/j.cyto.2019.154853.
Kolanis S, Vasileiou E, Hatzipantelis E, et al. Safety and efficacy of eltrombopag in children and adults with immune thrombocytopenia: a systematic review and meta-analysis[J]. Cardiovasc Hematol Agents Med Chem, 2021, 19(1): 83-92. PMID: 32914722. DOI: 10.2174/1871525718666200910161540.
Lassandro G, Palladino V, Vecchio GCD, et al. Thrombopoietin receptor agonists in children with immune thrombocytopenia: a new therapeutic era[J]. Endocr Metab Immune Disord Drug Targets, 2021, 21(3): 397-406. PMID: 32473624. DOI: 10.2174/1871530320666200531142244.
Han S, Zhu T, Ding S, et al. Early growth response genes 2 and 3 induced by AP-1 and NF-κB modulate TGF-β1 transcription in NK1.1- CD4+ NKG2D+ T cells[J]. Cell Signal, 2020, 76: 109800. PMID: 33011290. DOI: 10.1016/j.cellsig.2020.109800.
