Interleukin-4 and interleukin-13 concentrations in bronchoalveolar lavage fluid in neonates with respiratory distress syndrome and concurrent ventilator-associated pneumonia
XIA Yao-Fang, LIU Cui-Qing, SHI Hui-Ju, MA Li.
Department of Neonatology, Hebei Province Children's Hospital, Shijiazhuang 050031, China.
Abstract：OBJECTIVE: To study the concentrations of IL-4 and IL-13 in bronchoalveolar lavage fluid (BALF) in neonates with respiratory distress syndrome (RDS) and concurrent ventilator-associated pneumonia (VAP). METHODS: Sixty-eight neonates with RDS undergoing mechanical ventilation for over 48 hrs were enrolled. IL-4 and IL-13 levels in BALF were measured using ELISA 1, 72 and 96 hrs after mechanical ventilation. The results were compared between the neonates with concurrent VAP (n=37) and without (n=31). RESULTS: The levels of BALF IL-4 96 hrs after ventilation in the VAP group (35.34±1.78 ng/mL) were significantly higher than those in the non-VAP group (13.69±2.47ng/mL, P<0.05). The levels of BALF IL-13 96 hrs after ventilation in the VAP group (33.74±2.74 ng/mL) also increased significantly compared with those in the non-VAP group (13.50±3.81 ng/mL) (P<0.05). There were significant differences in BALF IL-4 and IL-13 levels between 1 hr and 96 hrs in the VAP group (P<0.05).ConclusionsBALF IL-4 and IL-13 levels increase in neonates with RDS and concurrent VAP. IL-4 and IL-13 may involve in the regulation of the inflammatory immue response.［Chin J Contemp Pediatr, 2009, 11 (8):645-648］
XIA Yao-Fang,LIU Cui-Qing,SHI Hui-Ju et al. Interleukin-4 and interleukin-13 concentrations in bronchoalveolar lavage fluid in neonates with respiratory distress syndrome and concurrent ventilator-associated pneumonia[J]. CJCP, 2009, 11(08): 645-648.
［1］Iwamura C, Kimura MY, Shinoda K, Endo Y, Hasegawa A, Yamashita M, et al. Schnurri-2 regulates Th2-dependent airway inflammation and airway hyperresponsiveness［J］.Int Immunol, 2007, 19(6):755-762.
［7］Liu Q, Liu Z, Rozo CT, Hamed HA, Alem F, Urban JF Jr, et al. The role of B cells in the development of CD4 effector T cells during a polarized Th2 immune response［J］.J Immunol, 2007, 179(6):3821-3830.
［8］Kay AB. The role of T lymphocytes in asthma［J］. Chem Immunol Allergy, 2006, 91:59-75.
［9］Kimura MY, Iwamura C, Suzuki A, Miki T, Hasegawa A, Sugaya K, et al. Schnurri-2 controls memory Th1 and Th2 cell numbers in vivo［J］. J Immunol, 2007, 178(8):4926-4936.
［10］Kimura MY, Hosokawa H, Yamashita M, Hasegawa A, Iwamura C, Watarai H, et al. Regulation of T helper type 2 cell differentiation by murine Schnurri-2［J］. J Exp Med, 2005, 201(3):397-408.
［11］Kellner J, Gamarra F, Welsch U, Jorres RA, Huber RM, Bergner A, et al. IL-13Ralpha2 reverses the effects of IL-13 and IL-4 on bronchial reactivity and acetylcholine-induced Ca+ signaling［J］. Int Arch Allergy Immunol, 2007, 142(3):199-210.
［12］Salahuddin N, Zafar A, Sukhyani L, Rahim S, Noor MF, Hussain K, et al. Reducing ventilator-associated pneumonia rates through a staff education programme［J］. J Hosp Infect, 2004, 57(3):223-227.
