Matrine inhibits the proliferation of neuroblastoma LAN5 cell and MYCN mRNA expression
FENG Chen, TANG Suo-Qin, WANG Jian-Wen, LONG Hui, YANG Guang.
Department of Pediatrics, People's Liberation Army General Hospital, Beijing 100853 , China (Tang S-Q, Email: suoqin@yahoo.com)
Abstract OBJECTIVE: Neuroblastoma is the most common malignant solid tumor in children under 4 years. Amplification of MYCN oncogene is associated with advanced-stage disease, rapid tumor progression, resistance to treatment, and poor outcome. Matirne has the anti-tumor activity. This study was designed to investigate the effects of matrine on LA-N-5 cell line proliferation and MYCN gene mRNA expression. METHODS: Neuroblastoma LA-N-5 cells were treated by 0.25,0.50,0.75 or 1.00 mg/mL matrine. MTT was used to measure the levels of the proliferation of LA-N-5 cells cultured with different concentrations of matrine. MYCN gene mRNA expression in LA-N-5 cells was measured using real time RT-PCR with SYBR GREEN I fluorescence. RESULTS: The proliferation of LA-N-5 cells was obviously inhibited by matrine in a dose-and time-dependent manner. Matrine of 1.00 mg/mL treatment for 72 hrs produced a best effect, with an inhibitory rate of LA-N-5 cell proliferation of 36.3% and an inhibitory rate of MYCN gene mRNA expression of 44.6%. CONCLUSIONS: Matrine may inhibit the growth of neuroblastoma cells and down-regulate MYCN mRNA expression. It may be promising as a new drug for treatment of neuroblastoma.
Key words ：
Matrine
Neuroblastoma
MYCN
Quantitative RT-PCR
Cell line
Cite this article:
FENG Chen,TANG Suo-Qin,WANG Jian-Wen et al. Matrine inhibits the proliferation of neuroblastoma LAN5 cell and MYCN mRNA expression[J]. 中国当代儿科杂志, 2008, 10(2): 225-227.
FENG Chen,TANG Suo-Qin,WANG Jian-Wen et al. Matrine inhibits the proliferation of neuroblastoma LAN5 cell and MYCN mRNA expression[J]. CJCP, 2008, 10(2): 225-227.
URL:
http://www.zgddek.com/EN/ OR http://www.zgddek.com/EN/Y2008/V10/I2/225
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