Abstract Objective 8-iso-8-iso-prostaglandin F 2α (8-iso-PGF 2α) is an index that can sensitively and specifically reflect peroxidation caused by increased free radicals after reperfusion. N-acetlycysteine (NAC) acts as an effective scavenger for free radical cleaning in the body. This study aimed to analyze the correlation of 8-iso-prostaglandin F 2α level between serum and myocardial tissue, and to investigate the therapeutic effect of NAC and the significance of serum 8-iso-PGF 2α level in rats with acute myocardial ischemia. Methods Forty-five male manhood Wistar rats were divided into three groups (15 rats in each group): Ischemia, NAC, and Control groups. Rats in the NAC group were given gastric lavage with NAC (0.1g/kg per day) for three weeks. Two weeks later the Ischemia and NAC groups were subjected to acute myocardial ischemia induced by intraperitoneal injection of pituitrin (20 U/kg), and the elevation of the ST segment in the ECG was used as the index of myocardial ischemia. The Control rats were injected intraperitoneally with normal saline. The 8-iso-PGF 2α contents in serum and myocardial tissue were determined by ELISA. Results Compared with the Control group, the 8-iso-PGF 2α contents in serum and myocardial tissue of the Ischemia group were significantly higher (60.4±13.7 pg/mL vs 187.4±45.8 pg/mL and 88.6±16.9 pg/mL vs 259.3±47.5 pg/g, both P< 0.01) In the NAC group, 8-iso-PGF 2α concentrations of serum and myocardial tissue were 88.2±16.4 pg/mL and 109.4±24.7 pg/g respectively, lower than those in the Ischemia group (P < 0.01). The 8-iso-PGF 2α levels of serum and myocardial tissue were positively correlated (r=0.865, P<0.01). In comparison with the controls, ST segments of ECG in rats with myocardial ischemia elevated and the peak of ST segments occurred 45 minutes after myocardial ischemia (0.34±0.05 mV) (P < 0.01). Pre-treatment with NAC improved myocardial ischemia (the elevation of ST segment was only 0.18±0.05 mV). Conclusions The level of 8-iso-PGF 2α increased in both serum and myocardial tissues of rats with acute myocardial ischemia. The serum level of 8-iso-PGF 2α can be used to evaluate the severity of myocardial ischemia. NAC reduces the free radical damage in myocardial tissues, and therefore, it may improve the amelioration of the heart during myocardial ischemia.
SHI Ling-Ling,XIAO Xin,LIU Yu et al. Significance of serum 8-iso-prostaglandin F2αlevel in acut emyocardial ischemia and the therapeutic effect o fN-acetylcysteine in rats[J]. 中国当代儿科杂志, 2005, 7(1): 8-11.
SHI Ling-Ling,XIAO Xin,LIU Yu et al. Significance of serum 8-iso-prostaglandin F2αlevel in acut emyocardial ischemia and the therapeutic effect o fN-acetylcysteine in rats[J]. CJCP, 2005, 7(1): 8-11.
