Abstract Objective To determine the diagnostic value of coxsackie virus B-RNA (CVB-RNA) assay by the PCR technique and to ident ify the viral pathogen of myocarditis. Methods CVB3 was injec ted into the abdominal cavity of 75 mice. The pathological examination and CVB-R NA assay by the PCR technique were done. CVB-RNA was measured by the PCR method in 145 children with myocarditis. The positive rates of CVB-RNA of 104 cases who se courses were within one month were compared with 41 cases whose courses were more than one month. Results Of the 75 mice, 64 (85.3%) had pathological change of myocarditis. CVB-RNA was positive in 40 out of the 75 mi ce (53.3%). The positive rate of CVB-RNA was 46.2% in the blood of the 1 45 patients with myocarditis. The positive rate of CVB-RNA in patients whose cou rses were within one month (56.7%) was higher than that of patients whose co urses were longer than one month (26.8%). Conclusions The sensitivity and reliability of CVB-RNA assay in the blood by the PCR technique t o indentify the viral pathogen of myocarditis are high. The positive rate is hig her in patients whose courses of illness within one month than whose more than o ne month.
GE Ming,MA Pei-Ran,WANG Wei et al. Value of Coxsackie Virus B-RNA Assay by the PCR Technique to Identify the Viral Pathogen of Myocarditis[J]. 中国当代儿科杂志, 2000, 2(5): 333-335.
GE Ming,MA Pei-Ran,WANG Wei et al. Value of Coxsackie Virus B-RNA Assay by the PCR Technique to Identify the Viral Pathogen of Myocarditis[J]. CJCP, 2000, 2(5): 333-335.
