Tumor Necrosis Factor-α in Brain Tissues of Rats with Pertussis Bacilli Induced Brain Edema
YIN Fei, YANG Yu-Jia, YU Pei-Lan, MAO Ding-An, TAO Yong-Guang
Department of Pediatrics, Xiangya Hospital, Hunan Medical University, Changsha 410008
Abstract Objective To understand the relationship between tumor necrotic factor α (TNF α) and brain damage in an animal model of pertussis bacilli induced brain edema. Methods 99 rats were divided into 3 groups: 1)sham control group (C group, n=11); 2)normal saline group (NS group, n=44); and 3) pertussis bacilli group (PB group, n=44). TNF α mRNA expression was examined using RT PCR and the protein levels were assayed using ELISA in the rat brain tissues at various times. Results TNF α was significantly increased in the PB group at 240 min ( 768.4 ± 86.3 pg/g) compared to the C group ( 318.1± 31.1 pg/g), NS group at 30 min ( 324.2 ± 65.6 pg/g), NS group at 60 min ( 322.1± 73.4 pg/g), NS group at 120 min ( 316.8 ± 49.1 pg/g), and NS group at 240 min ( 329.9 ± 77.6 pg/g). The PB group at 240 minutes was also significantly increased compared to the PB group at 30 min ( 360.3 ± 66.6 pg/g), at 60 min ( 358.5 ± 49.1 pg/g), and at 120 min ( 356.0 ± 52.3 pg/g) (P< 0.01 ). Expression of TNF α mRNA began to increase in the PB group at 60 min and reached a peak at 240 min. Conclusions TNF α may play a role in producing injury in infectious brain edema.
Key words ：
Brain edema
TNF-α
Pertussis bacilli
Rat
Cite this article:
YIN Fei,YANG Yu-Jia,YU Pei-Lan et al. Tumor Necrosis Factor-α in Brain Tissues of Rats with Pertussis Bacilli Induced Brain Edema[J]. 中国当代儿科杂志, 2000, 2(2): 82-85.
YIN Fei,YANG Yu-Jia,YU Pei-Lan et al. Tumor Necrosis Factor-α in Brain Tissues of Rats with Pertussis Bacilli Induced Brain Edema[J]. CJCP, 2000, 2(2): 82-85.
URL:
http://www.zgddek.com/EN/ OR http://www.zgddek.com/EN/Y2000/V2/I2/82
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