Abstract OBJECTIVE: To study the changes of glucocorticoid receptor (GR) expression in embryonic rat cortical neurons exposed to transient Mg2+-free treatment. METHODS: Six days after rat cortical neuronal cultures, two groups were created based on the medium to which were transiently exposed. The control group was exposed to a physiological solution (PS), and the Mg2+-free group was exposed to the same medium as the control group except for the removal of magnesium. The expression of GR mRNA and protein was determined by real-time PCR and immunocytochemistry staining 1, 7 and 12 days after transient Mg2+-free treatment. RESULTS: Compared to the control group, the Mg2+-free group displayed the significantly less accumulated optical density (AOD) of GR immunoreactivity 12 days after transient Mg2+-free treatment (P<0.05). On the contrary, GR mRNA expression increased significantly 1 and 7 days after transient Mg2+-free treatment in the Mg2+-free group (P<0.05). CONCLUSIONS: GR expression is modified following Mg-free-induced injury in cultured developing neurons in rats.［Chin J Contemp Pediatr, 2010, 12 (3):211-214］
BO Tao,YI Lu,WANG Tuan-Mei et al. Effect of magnesium-free on glucocorticoid receptor expression in primary cultured cortical neurons of fetal rats in vitro[J]. 中国当代儿科杂志, 2010, 12(3): 211-214.
BO Tao,YI Lu,WANG Tuan-Mei et al. Effect of magnesium-free on glucocorticoid receptor expression in primary cultured cortical neurons of fetal rats in vitro[J]. CJCP, 2010, 12(3): 211-214.
［2］Hauwal M, Furon E, Canova C, Griffths M, Neal J, Gasque P. Innate (inherent) control of brain infection, brain inflammation and brain repair: the role of microglia, astrocytes, "protective" glial stem cells and stromal ependymal cells ［J］. Brain Res Rev, 2005, 48 (2):220-233.
［3］Milla R, Riitta P, Helena L, Liisa L, Leena H. Interleukin-6-174 and-572 genotypes and the volume of deep gray matter in preterm infants ［J］. Pediatr Res, 2009, 65(1): 90-96.
［4］Dammon O, Matthews SG. The effects of repeated antenatal glucocorticoid therapy in the developing brain［J］. Pediatr Res, 2001, 50 (5):563-564.
［5］Cao HY, Jiang YW, Liu ZW, Wu XR. Effect of recurrent epileptiform discharges induced by magnesium-free treatment on developing cortical neurons in vitro［J］. Dev Brain Res, 2003, 142 (1):1-6.
［6］Altura BM, Gebrewold A, Zhang A, Altura BT. Low extracellular magnesium ions induce lipid perioxidation and activation of nuclear factor-kappa B in canine cerebral vascular smooth muscle: possible relation to traumatic brain injury and strokes［J］. Neurosci Lett, 2003, 341(3):189-192.
［9］Donley MP, Schμlkin J, Rosen JB. Glucocorticoid receptor antagonism in the basolateral amygdala and ventral hippocampus interferes with long-term memory of contextual fear［J］. Behav Brain Res, 2005, 164(2):197-205.
［10］Velísek L. Prenatal corticosteroid impact on hippocampus: implication for postnatal outcomes ［J］. Epliepsy Behav, 2005, 7(1): 57-67.
［11］Han F, Ozawa H, Matsuda K, Nishi M, Kawata M. Colocafization of mineralocorticoid receptor and glucocorticoid receptor in the hippocampus and hypothalamus ［J］. Neurosci Res, 2005, 51(4): 371-381.
［12］Pryce CR, Feldon J, Fuchs E, Knuesel I, Oertle T, Sengstag C, et al. Postnatal ontogeny of hippocampal expression of the mineralocorticoid and glucocorticoid receptor in the common monkey ［J］. Eur J Neurosci, 2005, 21(6): 1521-1535.
［13］Donley MP, Schulkin J, Rosen JB. Glucocorticoid receptor antagonism in the basolateral amygdala and ventral hippocampus interferes with long-term memory of contextual fear ［J］. Behav Brain Res, 2005, 164(2):197-205.
［14］Nishi M, Kawata M. Dynamics of glucocorticoid receptor and mineralocorticoid receptor: implications from live cell imaging studies ［J］. Neuroendocrinology, 2007, 85(3):186-192.
［15］Marin F, Pozzto C, Andreetta V, Jansson B, Arban R, Domenici E, et al. Single exposure to social defeat increases corticotropin-releasing factor and glucocorticoid receptor on RNA expression in rat hippocampus［J］. Brain Res, 2006, 1067(1):25-35.
［16］Tamura G ,Olson D, Miron J, Clark TG. Tolloid-like I is negatively regulated by stress and glucocorticoid［J］. Brain Res Mol Brain Res, 2005, 142(2):81-90.
［17］Isgor C, Kabbaj M, Akil H, Watson SJ. Delayed effects of chronic variable stress during peripubertal juvenile period on hippocampal morphology and on cognitive and stress axis functions in rats［J］. Hippocampus, 2004, 14(5):636-648.
［18］Yang CH, Huang CC, Hsu KS. Behavioral stress modifies hippocampal synaptic plasticity through corticosterone-induced sustained extracellular signal-regulated Kinase/mitogen-activated protein Kinase activation［J］. J Neurosci, 2004, 24(49):11029-11034.
