Effects of recombinant human erythropoietin on serum levels of neuron-specific enolase, S-100β protein and myelin basic protein in rats following status epilepticus
JIANG Chun-Ming, DU Jian-Min, LIU Zhan-Li, CHEN Li-Qiong, FENG Mei, YANG Yi-Hua, WANG Jie-Hua, HUANG Xian-Mei
Department of Pediatrics, Hangzhou People's Hospital Affiliated to Nanjing Medical University, Hangzhou 310006, China. Email: hxianmei@hotmail.com
Abstract OBJECTIVE: This study examined the effect of recombinant human erythropoietin (r-HuEPO) on the serum levels of neuron-specific enolase (NSE), S-100β protein and myelin basic protein (MBP) in young rats 24 hrs after lithium-pilocarpine-induced status epilepticus (SE) in order to study the potential role of r-HuEPO in epileptic brain damage. METHODS: Forty 19-21-day-old male Sprague-Dawley (SD) rats were randomly divided into four groups (n=10): normal control group, SE, r-HuEPO pretreated-SE and r-HuEPO. SE was induced by lithium-pilocarpine. R-HuEPO (500 IU/kg) was intraperitoneally injected in the r-HuEPO pretreated-SE and r-HuEPO groups 4 hrs before SE. Serum levels of NSE, S-100β and MBP were determined 24 hrs after the SE event. RESULTS: Serum levels of NSE, S-100β and MBP in the SE group increased significantly compared with those in the normal control and the r-HuEPO groups (P＜0.05). The r-HuEPO pretreated-SE group showed significantly decreased serum levels of NSE, S-100β and MBP compared with the SE group (P＜0.05). CONCLUSIONS: r-HuEPO may reduce the expression of NSE, S-100β and MBP and thus might provide an early protective effect against epileptic brain injury.
JIANG Chun-Ming,DU Jian-Min,LIU Zhan-Li et al. Effects of recombinant human erythropoietin on serum levels of neuron-specific enolase, S-100β protein and myelin basic protein in rats following status epilepticus[J]. 中国当代儿科杂志, 2011, 13(1): 50-52.
JIANG Chun-Ming,DU Jian-Min,LIU Zhan-Li et al. Effects of recombinant human erythropoietin on serum levels of neuron-specific enolase, S-100β protein and myelin basic protein in rats following status epilepticus[J]. CJCP, 2011, 13(1): 50-52.
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