Abstract OBJECTIVE: To study the effect of maternal vitamin D deficiency on lung morphogenesis and platelet-derived growth factor-A (PDGF-A) expression in rat offspring. METHODS: Sprague-Dawley (SD) female rats were randomly divided into two groups: normal control and vitamin D deficiency, with 6 rats in each group. The vitamin D deficiecy group was kept away from light and fed with the forage without vitamin D. After 2 weeks, the rats were mated with normal SD male rats. The morphological changes of fetal rat lungs on day 20 of gestation and 1-day-old neonatal rat lungs were observed by light microscope and electronic microscope. The levels of PDGF-A mRNA and protein in fetal and neonatal rat lungs were measured by reverse transcriptase-polymerase chain reaction (RT-PCR) technique and Western blot method respectively. RESULTS: Under the light microscope, smaller alveolar space, smaller diameter of the respiratory membrane and thicker alveolus mesenchyma were observed in lungs of fetal and neonatal rats from the vitamin D deficiency group compared with the controls (P<0.05). Under the electronic microscope, fewer lamellar bodies but more glycogen deposition in intracytoplasm were observed in the lungs of fetal rats from the vitamin D deficiency group compared with the controls. There was an increased number of empty lamellar bodies in neonatal rats from the vitamin D deficiency group. The levels of PDGF-A mRNA and protein in lungs of fetal and neonatal rats from the vitamin D deficiency group were significantly lower than the controls (P<0.05). CONCLUSIONS: Maternal vitamin D deficiency during pregnancy may inhibit the development of lung morphogenesis and PDGF-A expression in late fetal and neonatal rats. The low expression of PDGF-A may be involved in the inhibitory effect of vitamin D deficiency on the lung development.
GU Hai-Yan,ZHAO De-Yu,WANG Quan. Effect of maternal vitamin D deficiency on lung morphogenesis and platelet-derived growth factor-A expression in rat offspring[J]. 中国当代儿科杂志, 2011, 13(4): 326-330.
GU Hai-Yan,ZHAO De-Yu,WANG Quan. Effect of maternal vitamin D deficiency on lung morphogenesis and platelet-derived growth factor-A expression in rat offspring[J]. CJCP, 2011, 13(4): 326-330.
［1］Marin L, Dufour ME, Nguyen TM, Tordet C, Garabedian M. Maturational changes induced by 1 alpha, 25-dihydroxyvitamin D3 in type II cells from fetal rat lung explants［J］. Am J Physiol, 1993, 265(1 Pt 1): L45-L52.
［2］Rehan VK, Torday JS, Peleg S, Gennaro L, Vouros P, Padbury J, et al. 1 alpha, 25-dihydroxy-3-epi-vitamin D3, a natural metabolite of 1 alpha, 25-dihydroxy vitamin D3: production and biological activity studies in pulmonary alveolar type II cells［J］. Mol Genet Metab, 2002, 76 (1): 46-56.
［3］Nguyen TM, Guillozo H, Marin L, Tordet C, Koite S, Garabedian M. Evidence for a Vitamin D paracrine system regulating maturation of developing rat lung epithelium［J］. Am J Physiol, 1996, 271(15): L392-L399.
［5］Gaultier C, Harf A, Balmain N, Cuisinier-Gleizes P, Mathieu H. Lung mechanics in rachitic rats［J］. Am Rev Respir Dis, 1984, 130(6): 1108-1110.
［6］Andiran N, Yordam N, Oz-n A. Risk factors for vitamin D deficiency in breast-fed newborns and their mothers［J］. Nutrition, 2002, 18(1): 47-50.
［7］Siddiqui TS. Respiratory distress: a rare presentation of rickets［J］. J Ayub Med Coll Abbottabad, 2006, 18(3): 67-68.
［8］Samtani MN, Pyszczynski NA, Dubois DC, Almon RR, Jusko WJ. Modeling glucocorticoid mediated fetal lung maturation: I. Temporal patterns of corticosteroids in rat pregnancy［J］. J Pharmacol Exp Ther, 2006, 317(1): 117-126.
［9］Nguyen M, Trubert CL, Rizk-Rabin M, Rehan VK, Besancon F, Cayre YE, et al. 1, 25-Dihydroxyvitamin D3 and fetal lung maturation: immunogold detection of VDR expression in pneumocytes type II cells and effect on fructose 1, 6 bisphosphatase［J］. J Steroid Biochem Mol Biol, 2004, 89-90(1-5):93-97.
［11］Li J, Hoyle GW. Overexpression of PDGF-A in the lung epithelium of transgenic mice produces a lethal phenotype associated with hyperplasia of mesenchymal cells［J］. Dev Biol, 2001, 239 (2): 338-349.
［12］Assaad AM, Kawut SM, Arcasoy SM, Rosenzweig EB, Wilt JS, Sonett JR, et al. Platelet-derived growth factor is increased in pulmonary capillary hemangiomatosis［J］. Chest, 2007, 131(3): 850-855.
［13］Pedigo N, Zhang H, Koszewski NJ, Kaetzel DM. A 5′-distal element mediates vitamin D-inducibility of PDGF-A gene transcription［J］. Growth Factors, 2003, 21(3-4):151-160.
［14］Pedigo NG, Zhang H, Mishra A, McCorkle JR, Ormerod AK, Kaetzel DM. Retinoic acid inducibility of the human PDGF-a gene is mediated by 5′-distal DNA motifs that overlap with basal enhancer and vitamin D response elements［J］. Gene Expr, 2007, 14(1): 1-12.
