Abstract OBJECTIVE: To study the effects of intestinal trefoil factor (ITF) on Toll-like receptors (TLR) 2 and 4 expression in intestinal tissue and on intestinal injury in young rats with endotoxemia. METHODS: A total of 24 10-day-old Wistar rat pups were equally randomly divided into three groups: a control group, intraperitoneally injected with normal saline 1 mL/kg; an endotoxemia group, intraperitoneally injected with lipopolysaccharide (LPS) 5 mg/kg and an ITF group,intraperitoneally injected with rITF 0.1 mL/each plus LPS 5 mg/kg. Rats were sacrificed 3 h after injection. A segment of distal ileum was dissected for pathologic examinations under an optical microscope (hematoxylin-eosin staning). The mRNA expressions of TLR2 and TLR4 were detected by reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: The structure of the small intestine remained normal in the control group. Edema of interstitial substance and epithelium were observed in both LPS and ITF groups, whereas such changes were significantly lower in the ITF group than in the LPS group. The ITF group had significantly higher TLR2 mRNA expression than the NS and LPS groups (P＜0.01), whereas the mRNA expression of TLR4 in the ITF group was significantly lower than in the NS and LPS groups (both P＜0.01). CONCLUSIONS: ITF can alleviate intestinal injury in young rats with endotoxemia, which may be related to the down-regulation of TLR4 mRNA expression.
JING Ke,SUN Mei. Effects of intestinal trefoil factor on Toll-like receptors 2 and 4 expression in intestinal tissue in young rats with endotoxemia[J]. 中国当代儿科杂志, 2011, 13(12): 985-988.
JING Ke,SUN Mei. Effects of intestinal trefoil factor on Toll-like receptors 2 and 4 expression in intestinal tissue in young rats with endotoxemia[J]. CJCP, 2011, 13(12): 985-988.
［2］Li J, Zhou R, He WC, Xia B. Effects of recombinant human intestinal trefoil factor on trinitrobenzene sulphonic acid induced colitis in rats［J］. Mol Biol Rep, 2011 38(7):4787-4792.
［3］Uematsu S, Akira S. Toll-like receptors and innate immunity［J］. J Mol Med, 2006, 84(9): 712-725.
［4］Peterson CY, Costantini TW, Loomis WH, Putnam JG, Wolf P, Bansal V, et al. Toll-like receptor-4 mediates intestinal barrier breakdown after thermal injury［J］. Surg Infect (Larchmt), 2010, 11(2): 137-144.
［15］Renes IB, Verburg M, Van Nispen DJ, Büller HA, Dekker J, Einerhand AW. Distinct epitherial responses in-experimental colitis: implications for ion uptake mucosal protection［J］. Am J Physiol Gastrointest Liver Physiol, 2002, 283(1):G169-G179.
［16］Dunzendorfer S, Lee HK, Soldau K, Tobias PS. Toll-like receptor 4 functions intracellularly in human coronary artery endothelial cells: roles of LBP and sCD14 in mediating LPS responses［J］. FASEB J, 2004, 18(10): 1117-1119.
［17］Ohnishi T, Muroi M, Tanamoto K.The lipopolysaccharide-recognition mechanism in cells expressing TLR4 and CD14 but lacking MD-2［J］. FEMS Immunol Med Microbiol, 2007, 51(1): 84-91.
［18］Ortega-Cava CF, Ishihara S, Rumi MA, Kawashima K, Ishimura N, Kazumori H, et al. Strategic compartmentalization of Toll-like receptor 4 in the mouse gut［J］. J Immunol, 2003, 170(8): 3977-3985.
［19］Uematsu S, Fujimoto K.The innate immune system in the intestine［J］. Microbiol Immunol. 2010,54(11):645-657.
［20］Miyake K.Innate recognition of lipopolysaccharide by CD14 and toll-like receptor 4-MD-2: unique roles for MD-2［J］. Int Immunopharmacol, 2003, 3(1): 119-128.
