Abstract OBJECTIVE: To study the role of neuroglobin (Ngb) in the pathologic process of contusion and laceration of brain in children. METHODS: The proteins in the brain tissue were extracted by two-dimensional gel electrophoresis in 3 children undergoing brain ventricular neoplasms resection (normal brain tissue) and in 8 children with contusion and laceration of brain. The image analysis was done using the PDQuest 7.0 software. The differential protein spots were detected and analyzed with Applied Biosystems Voyager System 4307 MALDI-TOF Mass Spectrometer and bioinformatical skills. Ngb expression in the brain tissue was measured using immunohistochemisty. Ngb expression in plasma was measured using ELISA in 15 children with contusion and laceration of brain and 10 healthy children. RESULTS: Expression maps of the brain tissue were established by two-dimensional gel electrophoresis in children with contusion and laceration of brain and healthy children. Six differential protein spots were found and 5 of them were identified by mass spectrum. Immunohistochemisty assay showed that Ngb expression in the brain tissue in children with contusion and laceration of brain was significantly higher than in normal controls (P<0.05). ELISA results showed that Ngb expression in the plasma increased significantly 6, 12, 18, 24 and 48 hours after trauma in children with contusion and laceration of brain compared with healthy children (P<0.01). CONCLUSIONS: Ngb may play an important role in the pathologic process of contusion and laceration of brain in children.
LI Zhen-Yan,DENG Zheng-Hao,LI Chun-Tao et al. Changes of neuroglobin in the pathologic process of contusion and laceration of brain in children[J]. 中国当代儿科杂志, 2012, 14(09): 697-702.
LI Zhen-Yan,DENG Zheng-Hao,LI Chun-Tao et al. Changes of neuroglobin in the pathologic process of contusion and laceration of brain in children[J]. CJCP, 2012, 14(09): 697-702.
［1］Greenberg DA, Jin K, Khan AA. Neuroglobin: an endogenous neuroprotctant［J］. Curr Opin Pharmacol, 2008, 8(1): 20-24.
［2］Wang X, Liu J, Zhu H, Tejima E, Tsuji K, Murata Y, et al. Effects of neuroglobin overexpression on acute brain injury and long-term outcomes after focal cerebral ischemia［J］. Stroke, 2008, 39(6): 1869-1874.
［7］Cantore L, Norwood K, Patrick P. Medical aspects of pediatric rehabilitation after moderate to severe traumatic brain injury［J］. NeuroRehabilitation, 2012, 30(3): 225-234.
［8］Brown FL, Whittingham K, Boyd R, Sofronoff K.J. A Systematic Review of Parenting Interventions for Traumatic Brain Injury: Child and Parent Outcomes［J］. Head Trauma Rehabil, 2012,［Epub ahead of print］.
［9］Sanchez JI，Paidas CN. Chijdhood trauma, now and in the new millennium［J］. Surg Clin North Am, 2004, 79(6): 1503-1508.
［10］Appelboom G, Zoller SD, Piazza MA, Szpalski C, Bruce SS, McDowell MM, et al. Traumatic brain injury in pediatric patients: evidence for the effectiveness of decompressive surgery［J］. Neurosurg Focus, 2011, 31(5): E5.
［11］Schober ME, Block B, Requena DF, Hale MA, Lane RH. Developmental traumatic brain injury decreased brain derived neurotrophic factor expression late after injury［J］. Metab Brain Dis, 2012, 27(2):167-173.
［12］Xu JF, Liu WG, Dong XQ, Yang SB, Fan J. Change in plasma gelsolin level after traumatic brain injury［J］. J Trauma Acute Care Surg, 2012, 72(2): 491-496.
