Abstract Objective To study the roles of CD4+CD25+Foxp3+ regulatory T cells (Treg) and IL-33 in the pathogenesis of asthma in children. Methods Flow cytometry was used to detect peripheral blood CD4+CD25+Foxp3+Treg proportion in CD4+T lymphocytes in.45 children with asthma, 50 children with wheezing caused by respiratory syncytial virus infection and 40 healthy children. Serum levels of IFN-γ, IL-4, IL-5 and IL-33 were measured using ELISA. Results The level of peripheral blood CD4+CD25+Foxp3+Treg in the asthma group was significantly lower than in the wheezing and control groups (P<0.05). In contrast, serum levels of IL-33 in the asthma group was significantly higher than in the wheezing and control groups (P<0.05). Peripheral blood CD4+CD25+Foxp3+Treg level was negatively correlated with serum IL-33 level in the asthma group(r=-0.156, P<0.01). Conclusions CD4+CD25+Foxp3+Treg may interact with IL-33 in the pathogenesis of childhood asthma.
PAN Zhen-Zhen,LI Ling,GUO Yun et al. Roles of CD4+CD25+Foxp3+ regulatory T cells and IL-33 in the pathogenesis of asthma in children[J]. CJCP, 2014, 16(12): 1211-1214.
PAN Zhen-Zhen,LI Ling,GUO Yun et al. Roles of CD4+CD25+Foxp3+ regulatory T cells and IL-33 in the pathogenesis of asthma in children[J]. CJCP, 2014, 16(12): 1211-1214.
Shimizu T, Kida Y, Kuwano K. TriacylaTed lipoproTeins derived from Mycoplasma pneumoniae acTivaTe nuclear facTor-kappa B Through Toll-like recepTors 1 and 2[J]. Immunology, 2007, 121(4): 473-483.
Zhang M, Qian YY, Chai SJ, eT al. Enhanced local Foxp3 expression in lung Tissue aTTenuaTes airway inflammaTion in a mouse model of asThma[J]. J AsThma, 2014, 51(5): 451-458.
[5]
Hardy RD, Jafri HS, Olsen K, eT al. ElevaTed cyTokine and chemokine levels and prolonged pulmonary airflow resisTance in a murine Mycoplasma pneumoniae pneumonia model: a microbiologic, hisTologic, immunologic, and respiraTory pleThysmographic profile[J]. InfecT Immun, 2001, 69(6): 3869-3876.
[6]
Baba S, Kondo K, Kanaya K, eT al. Expression of IL-33 and iTs recepTor ST2 in chronic rhinosinusiTis wiTh nasal polyps[J]. Laryngoscope, 2014, 124(4): E115-E122.
[7]
STelmaszczyk-Emmel A, Zawadzka-Krajewska A, Szypowska A, eT al. Frequency and acTivaTion of CD4+CD25 Foxp3+ regulaTory T cells in peripheral blood from children wiTh aTopic allergy[J]. InT Arch Allergy Immunol, 2013, 162(1): 16-24.
Licona-Limón P, Kim LK, Palm NW, eT al. Th2, allergy and group 2 innaTe lymphoid cells[J]. NaT Immunol, 2013, 14(6): 536-542.
[10]
Saglani S, Lui S, Ullmann N, eT al. IL-33 promoTes airway remodeling in pediaTric paTienTs wiTh severe sTeroid-resisTanT asThma[J]. J Allergy Clin Immunol, 2013, 132(3): 676-685.
Ayyoub M, Raffin C, Valmori D. GeneraTion of Th17 from human naive CD4+ T cells preferenTially occurs from FOXP3+ Tregs upon cosTimulaTion via CD28 or CD5[J]. Blood, 2012, 119(20): 4810-4812.
