Abstract Objective To investigate the protective effect of emodin in young rats with intrahepatic cholestasis.Methods A total of 120 young Sprague-Dawley rats were randomly divided into control, model, and high-, medium-, and low-dose emodin groups, with 24 rats in each group.The rats in the control and model groups were given sodium carboxymethyl cellulose solution by gavage, while the other groups were given different doses of emodin solution by gavage.On the 5th day of experiment, alpha-naphthylisothiocyanate(ANIT, 50 mg/kg) was applied by gavage to establish the model of intrahepatic cholestasis in all groups except the control group.At 24, 48, and 72 hours after gavage, 8 rats in each group were sacrificed.Colorimetry was used to measure the serum levels of total bilirubin(TBIL), direct bilirubin(DBIL), total bile acid(TBA), alkaline phosphatase(ALP), gamma glutamyl transpeptidase(GGT), alanine aminotransferase(ALT), and aspartate aminotransferase(AST) in each group, and hematoxylin-eosin staining was applied to observe the morphological changes of the liver under a light microscope at different time points.Results Compared with the control group, the model group had significantly increased serum levels of TBIL, DBIL, TBA, ALP, GGT, ALT, and AST at the 24-hour, 48-hour, and 72-hour time points(PPPPConclusions Emodin can effectively improve ANIT-induced intrahepatic cholestasis in young rats, and medium-dose emodin shows the best effect.
XIONG Xiao-Li,YAN Su-Qi,QIN Huan et al. Protective effect of emodin pretreatment in young rats with intrahepatic cholestasis[J]. CJCP, 2016, 18(2): 165-171.
XIONG Xiao-Li,YAN Su-Qi,QIN Huan et al. Protective effect of emodin pretreatment in young rats with intrahepatic cholestasis[J]. CJCP, 2016, 18(2): 165-171.
Diniz G, Tosun Yildirim H, Calkavur S, et al. Can neonatal hepatitis be more fatal than biliary atresia?[J]. Fetal Pediatr Pathol, 2015, 34(3):162-168.
[4]
Mousa HS, Lleo A, Invernizzi P, et al. Advances in pharmacotherapy for primary biliary cirrhosis[J]. Expert Opin Pharmacother, 2015, 16(5):633-643.
Zhao YL, Wang JB, Zhou GD, et al. Investigations of free anthraquinones from rhubarb against alphanaphthylisothiocyanate-induced cholestatic liver injury in rats[J]. Basic Clin Pharmacol Toxicol, 2009, 104(6):463-469.
[8]
Ding Y, Zhao L, Mei H, et al. Exploration of emodin to treat alpha-naphthylisothiocyanate-induced cholestatic hepatitis via anti-inflammatory pathway[J]. Eur J Pharmacol, 2008, 590(1-3):377-386.
Jean PA, Roth RA. Naphthylisothiocyanate disposition in bile and its relationship to liver glutathione and toxicity[J]. Biochem Pharmacol, 1995, 50(9):1469-1474.
