Abstract Objective To investigate the immunoregulatory effect of adipose-derived stem cell (ADSC) transplantation by intraperitoneal injection in food-allergic young mice before and after ovalbumin (OVA) sensitization. Methods Thirty-two 3-week-old female Balb/c mice were randomly divided into control, allergic model, ADSC treatment, and ADSC prevention groups (n=8 each). A young mouse model of food allergy was established by OVA sensitization via intraperitoneal injection. Each mouse from the prevention and treatment groups was transplanted with 1×106 ADSCs on days 1 and 15 post-sensitization, respectively. The allergic symptoms of all groups were observed and scored. The jejunal villi and inflammatory cell infiltration were observed by hematoxylin-eosin staining. Serum levels of allergy-related inflammatory cytokines were measured by Luminex. Results Compared with the allergic model group, the ADSC prevention and ADSC treatment groups had significantly reduced allergic symptom scores (PPPConclusions ADSC transplantation, performed before or after sensitization, has an immunoregulatory effect on food allergy in young Balb/c mice, but this effect is better if transplantation is performed after sensitization.
LAI Yan-Ru,ZHENG Cheng-Zhong. Immunoregulatory effect of adipose-derived stem cell transplantation in young mouse model of food allergy[J]. CJCP, 2016, 18(7): 656-661.
LAI Yan-Ru,ZHENG Cheng-Zhong. Immunoregulatory effect of adipose-derived stem cell transplantation in young mouse model of food allergy[J]. CJCP, 2016, 18(7): 656-661.
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