Abstract Objective To investigate the effect of respiratory syncytial virus (RSV)-related pulmonary infection on endogenous metabolites in large intestinal mucosa in BALB/c mice using metabolomics technology based on gas chromatography-mass spectrometry (GC-MS). Methods Mice were randomly divided into a control group and a RSV pneumonia model group (n=16 each). The mouse model of RSV pneumonia was established using intranasal RSV infection (100×TCID50, 50 μL/mouse, once a day). After 7 days of intranasal RSV infection, the mice were sacrificed and GC-MS was used to identify endogenous metabolites and measure the changes in their relative content in colon tissue. SMCA-P12.0 software was used to perform principal component analysis (PCA) and orthogonal partial least squaresdiscriminant analysis (OPLS-DA) for endogenous metabolites in colon tissue. The differentially expressed metabolites in colon tissue were imported into the metabolic pathway platform Metaboanalyst to analyze related metabolic pathways. Results PCA and OPLS-DA showed significant differences between the control and RSV pneumonia model groups. A total of 32 metabolites were identified in the colon tissue of the mice with RSV pneumonia. The RSV pneumonia model group had significant increases in the content of leucine, isoleucine, glycine, alanine, arachidonic acid, and lactic acid, which were related to the valine, leucine, isoleucine, arachidonic acid, and pyruvic acid metabolic pathways. Conclusions RSV pneumonia might cause metabolic disorders in the large intestinal tissue in mice.
MENG Xin,WANG Shou-Chuan,SHAN Jin-Jun et al. Effect of respiratory syncytial virus-related pulmonary infection on endogenous metabolites in large intestinal mucosa in mice[J]. CJCP, 2016, 18(11): 1166-1173.
MENG Xin,WANG Shou-Chuan,SHAN Jin-Jun et al. Effect of respiratory syncytial virus-related pulmonary infection on endogenous metabolites in large intestinal mucosa in mice[J]. CJCP, 2016, 18(11): 1166-1173.
Torgerson DG,Giri T,Druley TE,et al.Pooled sequencing of candidate genes implicates rare variants in the development of asthma following severe RSV bronchiolitis in infancy[J].PLoS One,2015,10(11):e0142649.
[2]
Chonmaitree T,Alvarez-Fernandez P,Jennings K,et al.Symptomatic and asymptomatic respiratory viral infections in the first year of life:association with acute otitis media development[J].Clin Infect Dis,2015,60(1):1-9.
[3]
Park A,Suh SI,Son GR,et al.Respiratory syncytial virusrelated encephalitis:magnetic resonance imaging findings with diffusion-weighted study[J].Neuroradiology,2014,56(2):163-168.
[4]
Chiu SN,Shao PL,Chen HC,et al.Risk of respiratory syncytial virus infection in cyanotic congenital heart disease in a subtropical area[J].J Pediatr,2016,171:25-30.e1.
Fernández-Rodríguez A,Ballesteros S,de Ory F,et al.Virological analysis in the diagnosis of sudden children death:a medico-legal approach[J].Forensic Sci Int,2006,161(1):8-14.
[7]
Wang J,Tian Z.How lung infection leads to gut injury[J].Oncotarget,2015,6(40):42394-42395.
[8]
Punyadarsaniya D,Winter C,Mork AK,et al.Precision-cut intestinal slices as a culture system to analyze the infection of differentiated intestinal epithelial cells by avian influenza viruses[J].J Virol Methods,2015,212:71-75.
[9]
Wang J,Li F,Wei H,et al.Respiratory influenza virus infection induces intestinal immune injury via microbiota-mediated Th17 cell-dependent inflammation[J].J Exp Med,2014,211(12):2397-2410.
[10]
Matsumoto H,Kimura Y,Murao T,et al.Severe colitis associated with both epstein-barr virus and cytomegalovirus reactivation in a patient with severe aplastic anemia[J].Case Rep Gastroenterol,2014,8(2):240-244.
Chen ZG,Luo H,Wang SC,et al.Antiviral effects of Jinxin oral liquid against respiratory syncytial virus infection in the BALB/c mice model[J].J Ethnopharmacol,2015,162:287-295.
[13]
Major HJ,Williams R,Wilson AJ,et al.A metabonomic analysis of plasma from Zucker rat strains using gas chromatography/mass spectrometry and pattern recognition[J].Rapid Commun Mass Spectrom,2006,20(22):3295-3302.
[14]
Munger J,Bajad SU,Coller HA,et al.Dynamics of the cellular metabolome during human cytomegalovirus infection[J].PLoS Pathog,2006,2(12):e132.
[15]
Semmo N,Weber T,Idle JR,et al.Metabolomics reveals that aldose reductase activity due to AKR1B10 is upregulated in hepatitis C virus infection[J].J Viral Hepat,2015,22(7):617-624.
[16]
Biolo G,Tessari P,Inchiostro S,et al.Leucine and phenylalanine kinetics during mixed meal ingestion:a multiple tracer approach[J].Am J Physiol,1992,262(4 Pt 1):E455-E463.
[17]
Hoerr RA,Matthews DE,Bier DM,et al.Effects of protein restriction and acute refeeding on leucine and lysine kinetics in young men[J].Am J Physiol,1993,264(4 Pt 1):E567-E575.
[18]
Riedijk MA,van Goudoever JB.Splanchnic metabolism of ingested amino acids in neonates[J].Curr Opin Clin Nutr Metab Care,2007,10(1):58-62.
Chen LX,Yin YL,Jobgen WS,et al.In vitro oxidation of essential amino acids by jejunal mucosal cells of growing pigs[J].Livest Sci,2007,109:19-23.
[21]
Chen L,Li P,Wang J,et al.Catabolism of nutritionally essential amino acids in developing porcine enterocytes[J].Amino Acids,2009,37(1):143-152.
[22]
Wilson CL,Ouellette AJ,Satchell DP,et al.Regulation of intestinal alpha-defensin activation by the metalloproteinase matrilysin in innate host defense[J].Science,1999,286(5437):113-117.