Abstract Objective To investigate the expression of intercellular adhesion molecule-1 (ICAM-1) in serum and induced sputum supernatant in children with bronchiolitis, as well as its role in the pathogenesis of bronchiolitis in children.Methods A total of 67 children with bronchiolitis who were diagnosed and hospitalized between July 2015 and January 2017 were enrolled. According to the diagnostic criteria, these children were divided into mild group with 22 children, moderate group with 24 children, and severe group with 21 children. A total of 20 children who underwent physical examination were enrolled as healthy control group. ELISA was used to measure the level of ICAM-1 in serum and induced sputum supernatant in the children with bronchiolitis in the acute stage and recovery stage and the children in the healthy control group.Results Compared with the healthy control group, the mild, moderate, and severe bronchiolitis groups had a significant increase in the level of ICAM-1 in serum and sputum (P<0.01). Compared with the mild group, the moderate and severe groups had a significant increase in the level of ICAM-1 in serum and sputum (P<0.01). Compared with the moderate group, the severe group had a significant increase in the level of ICAM-1 in serum and sputum (P<0.01). Compared with the children with bronchiolitis in the acute stage, the children in the recovery stage had a significant reduction in the level of ICAM-1 in serum and sputum (P<0.01). The correlation analysis showed that in the acute stage, the level of ICAM-1 in serum was positively correlated with that in sputum in children with bronchiolitis (r=0.875,P<0.001).Conclusions ICAM-1 is involved in the pathogenesis of bronchiolitis and is associated with disease severity.
ZHANG Xiao-Ming,ZHU Hai-Tao,CHANG Ming. Expression of vascular intercellular adhesion molecule-1 and its significance in children with bronchiolitis[J]. CJCP, 2018, 20(1): 43-47.
ZHANG Xiao-Ming,ZHU Hai-Tao,CHANG Ming. Expression of vascular intercellular adhesion molecule-1 and its significance in children with bronchiolitis[J]. CJCP, 2018, 20(1): 43-47.
Ralston SL, Lieberthal AS, Meissner HC, et al. Clinical practice guideline:the diagnosis, management, and prevention of bronchiolitis[J]. Pediatrics, 2014, 134(5):e1474-e1502.
[2]
Schroeder AR, Mansbach JM. Recent evidence on the management of bronchiolitis[J]. Curr Opin Pediatr, 2014, 26(3):328-333.
[3]
Sigurs N, Bjarnason R, Sigurbergsson F, et al. Respiratory syncytial virus bronchiolitis in infancy is an important risk factor for asthma and allergy at age 7[J]. Am J Respir Crit Care Med, 2000, 161(5):1501-1507.
Williamson JD, Sadofsky LR, Crooks MG, et al. Bleomycin increases neutrophil adhesion to human vascular endothelial cells independently of upregulation of ICAM-1 and E-selectin[J]. Exp Lung Res, 2016, 42(8-10):397-407.
[7]
Yang JX, Pan YY, Ge JH, et al. Tanshinone Ⅱ A attenuates TNF-α-induced expression of VCAM-1 and ICAM-1 in endothelial progenitor cells by blocking activation of NF-κB[J]. Cell Physiol Biochem, 2016, 40(1-2):195-206.
[8]
Kim H, Hwang JS, Woo CH, et al. TNF-alpha-induced up-regulation of intercellular adhesion molecule-1 is regulated by a Rac-ROS-dependent cascade in human airway epithelial cells[J]. Exp Mol Med, 2008, 40(2):167-175.
[9]
Benzaquen M, Lebowitz D, Belenotti P, et al. Acute pancreatitis and pneumonia due to Mycoplasma pneumoniae:a case report[J]. BMC Res Notes, 2016, 9:397.
[10]
Williamson PA, Short PM, Vaidyanathan S, et al. Inhaled and systemic corticosteroid response in severe asthma assessed by alveolar nitric oxide:a randomized crossover pilot study of add-on therapy[J]. Br J Clin Pharmacol, 2013, 75(1):93-102.
Nandan D, Kansal P, Patharia N, et al. Induced sputum nitrite levels correlate with clinical asthma parameters in children aged 7-18 years with mild to moderate persistent asthma[J]. J Lab Physicians, 2016, 8(2):90-95.
Faber TE, Groen H, Welfing M, et al. Specific increase in local IL-17 production during recovery from primary RSV bronchiolitis[J]. J Med Virol, 2012, 84(7):1084-1088.
[17]
Humlicek AL, Pang L, Look DC. Modulation of airway inflammation and bacterial clearance by epithelial cell ICAM-1[J]. Am J Physiol Lung Cell Mol Physiol, 2004, 287(3):L598-L607.
[18]
Gong JH, Shin D, Han SY, et al. Kaempferol suppresses eosionphil infiltration and airway inflammation in airway epithelial cells and in mice with allergic asthma[J]. J Nutr, 2012, 142(1):47-56.
[19]
Springer TA. Adhesion receptors of the immune system[J]. Nature, 1990, 346(6283):425-434.
[20]
Tang HP, Sun LX, Han W. Endothelial cells on the proliferation and expression of intercellular adhesion molecule 1 and interleukin 8 of vascular smooth muscle cells[J]. Genet Mol Res, 2013, 12(4):4363-4370.
[21]
Yen FL, Tsai MH, Yang CM, et al. Curcumin nanoparticles ameliorate ICAM-1 expression in TNF-α-treated lung epithelial cells through p47(phox) and MAPKs/AP-1 pathways[J]. PLoS One, 2013, 8(5):e63845.
[22]
Rafiee L, Hajhashemi V, Javanmard SH. Maprotiline inhibits LPS-induced expression of adhesion molecules (ICAM-1 and VCAM-1) in human endothelial cells[J]. Res Pharm Sci, 2016, 11(2):138-144.
[23]
Shcheglovitova ON, Skliankina NN, Babaiants AA, et al. Adhesion molecules expressed in vascular endothelial cells in natural immunity against viral infections[J]. Vestn Ross Akad Med Nauk, 2011, (10):54-60.
Ciebiada M, Gorska-Ciebiada M, Gorski P. sICAM-1 and TNF-α in asthma and rhinitis:relationship with the presence of atopy[J]. J Asthma, 2011, 48(7):660-666.
[26]
Traub S, Nikonova A, Carruthers A, et al. An anti-human ICAM-1 antibody inhibits rhinovirus-induced exacerbations of lung inflammation[J]. PLoS Pathog, 2013, 9(8):e1003520.
