Abstract OBJECTIVE: To study the expression of human β-defensin-3 (hBD-3) induced by lipopolysaccharide (LPS) in human bronchial epithelial (HBE) cells, and explore the role of hBD-3 in respiratary infection. METHODS: HBE cells were stimulated with different concentrations of LPS (0.01, 0.1, 1 and 10 μg/mL). hBD-3 mRNA expression was detected by RT-PCR 2 hrs later. hBD-3 protein expression was detected by Western blot 4 hrs later. RESULTS: hBD-3 mRNA and protein was weakly expressed in normal HBE cells. LPS stimulation resulted in a significant increase of hBD-3 mRNA and protein expression (P<0.01). hBD-3 mRNA and protein expression increased with increasing LPS concentrations. There were significant differences in the hBD-3 mRNA and protein expression in cells stimulated by different concentrations of LPS (P<0.05). CONCLUSIONS: LPS can induce hBD-3 expression in a dose-dependent manner. hBD-3 might play a role in initial defensive reaction against bacterial invasion.[Chin J Contemp Pediatr, 2009, 11 (7):577-580]
LI Jia,ZHANG Bing,ZHONG Li-Li. Expression of human β-defensin-3 induced by lipopolysaccharide in human bronchial epithelial cells[J]. 中国当代儿科杂志, 2009, 11(07): 577-580.
LI Jia,ZHANG Bing,ZHONG Li-Li. Expression of human β-defensin-3 induced by lipopolysaccharide in human bronchial epithelial cells[J]. CJCP, 2009, 11(07): 577-580.
[1]Schroder JM. Epithelial antimicrobial peptides: innate local host response elements[J]. Cell Mol Life Sci, 1999, 56(1-2): 32-46.
[2]Schroder JM, Harder J. Human beta-defensin-2[J]. Int J Biochem Cell Biol, 1999, 31(6):645-651.
[3]Harder J, Bartels J, Christophers E, Schroder JM. Isolation and characterization of human beta-defensin-3, a novel human inducible peptide antibiotic[J]. J Biol Chem, 2001, 276(8): 5707-5713.
[4]Jia HP, Schutte BC, Schudy A,Linzmeier R, Guthmiller JM, Johnson GK, et al. Discovery of new human beta-defensins using a genomicsbased approach[J]. Gene, 2001, 263(1-2): 211-218.
[5]Sahly H, Schubert S, Harder J, Rautenberg P, Ullmann U, Schroder J, et al. Burkholderia is highly resistant to human beta-defensin 3[J]. Antimicrob Agents Chemother, 2003, 47(5): 1739-1741.
[6]Schibli DJ, Hunter HN, Aseyev V, Starner TD, Wiencek JM, McCray PB Jr, et al. The solution structures of the human betadefensins lead to a better understanding of the potent bactericidal activity of HBD3 against Staphylococcus aureus[J]. J Biol Chem, 2002, 277(10): 8279-8289.
[7]Ishimoto H, Mukae H, Date Y, Shimbara T, Mondal MS, Ashitani J, et al. Identification of hBD-3 in respiratory tract and serum: the increase in pneumonia[J]. Eur Respir, 2006, 27(2): 253-260.
[9]Varoga D, Pufe T, Harder J, Schroder JM, Mentlein R, Meyer-Hoffert U, et al. Human β-defensin-3 mediates tissue remodeling processes in articular cartilage by increasing levels of metalloproteinases and reducing levels of their endogenous inhibitors[J]. Arthritis Rheum, 2005, 52(6): 1736-1745.
[10]Feng Z, Jiang B, Chandra J, Ghannoum M, Nelson S, Weinberg A. Human beta-defensins:differential activity against candidal species and regulation by Candida albicans[J]. Dent Res, 2005, 84(5):445-450.