Abstract OBJECTIVE: To study the effects of quercetin, a flavonoid, on the learning and memory ability of 3-day-old neonatal rats with hypoxic-ischemic brain white matter damage (WMD). METHODS: Sixty 3-day-old Sprague-Dawley rats were randomly divided into four groups: control, WMD model,and quercetin treatment groups (20 and 40 mg/kg). There were 15 rats in each group. Rats in the WMD model and the two quercetin treatment groups were subjected to right common carotid artery ligation followed by 2 hrs of exposure to 8% O2 to induce periventricular white matter injury. After the operation quercetin was administered daily in the two quercetin treatment groups for 6 weeks. Six weeks later, Morris water maze and open-field tests were carried out to test memory and learning ability as well as behavior and cognition. RESULTS: From the second day of training, escape latency in the Morris water maze test was more prolonged in the WMD model group than in the control group (P<0.01). The escape latency in the two quercetin treatment groups was shortened significantly compared with the WMD model group (P<0.05). The WMD model group crossed the original platform fewer times compared with the control and quercetin treatment groups (P<0.05). The open-field test indicated that the number of rearings increased and time spent in the centre was extended in the WMD model group compared with the control group. Compared with the WMD model group, the number of rearings was significantly reduced (P<0.05) and time spent in the centre was significantly shortened in the quercetin treatment groups (P<0.05). CONCLUSIONS: Quercetin treatment can improve memory and learning ability as well as cognitive ability in neonates with WMD, suggesting that quercetin protects against WMD resulting from hypoxia-ischemia.
HUANG Jing-Jing,LIU Xuan,WANG Xing-Qi et al. Effects of quercetin on the learning and memory ability of neonatal rats with hypoxic-ischemic brain damage[J]. 中国当代儿科杂志, 2012, 14(06): 454-457.
HUANG Jing-Jing,LIU Xuan,WANG Xing-Qi et al. Effects of quercetin on the learning and memory ability of neonatal rats with hypoxic-ischemic brain damage[J]. CJCP, 2012, 14(06): 454-457.
［1］Sundrum R, Logan S, Wallace A, Spencer N. Cerebral palsy and socioeconomic status: a retrospective cohort study［J］. Arch Dis Child, 2005: 90(1): 15-18.
［2］Cai Z, Pang Y, Xiao F, Rhodes PG. Chronic ischemia preferentially causes white matter injury in the neonatal rat brain［J］. Brain Res, 2001, 898(1): 126-135.
［3］Kamaraj S, Vinodhkumar R, Anandakumar P, Jagan S, Ramakrishnan G, Devaki T. The effects of quercetin on antioxidant status and tumor markers in the lung and serum of mice treated with benzo(a)pyrene［J］. Biol Pharm Bull, 2007, 30(12): 2268-2273.
［4］Kumar A, Sehgal N, Kumar P, Padi SS, Naidu PS. Protective effect of quercetin against ICV colchicine-induced cognitive dysfunctions and oxidative damage in rats［J］. Phytother Res, 2008, 22(12): 1563-1569.
［5］Oz-elik B, Kartal M, Orhan I.Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids［J］. Pharm Biol, 2011, 49(4): 396-402.
［8］Wang XQ, Yao RQ, Liu X, Huang JJ, Qi DS, Yang LH. Quercetin protects oligodendrocyte precursor cells from oxygen/glucose deprivation injury in vitro via the activation of the PI3K/Akt signaling pathway［J］. Brain Res Bull, 2011, 86(3-4): 277-284.
［10］Back SA, Luo NL, Borenstein NS, Levine JM, Volpe JJ, Kinney HC. Late oligodendrocyte progenitors coincide with the developmental window of vulnerability for human perinatal white matter injury［J］. J Neurosci, 2001, 21(4): 1302-1312.
［11］Pu F, Mishima K, Irie K, Motohashi K, Tanaka Y, Orito K, et al. Neuroprotective effects of quercetin and rutin on spatial memory impairment in an 8-arm radial maze task and neuronal death induced by repeated cerebral ischemia in rats［J］. J Pharmacol Sci, 2007, 104(4): 329-334.
［12］Liu J, Yu H, Ning X. Effect of quercetin on chronic enhancement of spatial learning and memory of mice［J］. Sci China C Life Sci, 2006, 49(6): 583-590.
［13］Lu J, Zheng YL, Luo L, Wu DM, Sun DX, Feng YJ. Quercetin reverses D-galactose induced neurotoxicity in mouse brain［J］. Behav Brain Res, 2006, 171(2): 251-260.
［14］Yao Y, Han DD, Zhang T, Yang Z. Quercetin improves cognitive deficits in rats with chronic cerebral ischemia and inhibits voltage-dependent sodium channels in hippocampal CA1 pyramidal neurons［J］. Phytother Res, 2010, 24(1): 136-140.
［15］Ahmad A, Khan MM, Hoda MN, Raza SS, Khan MB, Javed H, et al. Quercetin protects against oxidative stress associated damages in a rat model of transient focal cerebral ischemia and reperfusion［J］. Neurochem Res, 2011, 36(8): 1360-1371.
