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2001 Vol. 3 No. 03
Published: 2001-03-15
CLINICAL RESEARCH
CLINICAL EXPERIENCE
CASE REPORT
REVIEW
SPECIALIST COMMENTARY
209
WEI Ke-Lun
A number of hot topics on emergency care of neonates in China
No abstract available
2001 Vol. 3 (03): 209-211 [
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CASE REPORT
211
WANG Yun, SHA Sha
Neonatal persistent truncus arteriosus
No abstract available
2001 Vol. 3 (03): 211-211 [
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JIANG Hong, ZHANG Yi-He
Tuberous sclerosis
No abstract available
2001 Vol. 3 (03): 231-231 [
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252
LIU Na-Ping, LI Yan, ZHOU Zhong-Shu
IDDM caused by consanguineous marriage(1 case)
No abstract available
2001 Vol. 3 (03): 252-252 [
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ORIGINAL ARTICLE
212
LI Yun, ZHOU Ai-Qing
Functional and Structural Changes in Patients with Pulmonary Hypertension Secondary to Congenital Heart Disease
OBJECTIVE: To investigate possible mechanisms resulting in pulmonary hypertension (PH) in patients with congenital heart disease (CHD). METHODS: Thromboxane B2/6keto Prostaglandin F1a (TXB2/6-K-PGF1a), Von Willebrand factor (VWF: Ag) and tissue plasminogen activator (tPA) plasma levels were measured. Changes in pulmonary vascular endothelial cells were assessed with light and electron microscopy. Ninety-nine children with CHD were divided into 3 groups according to pulmonary vascular resistance (PVR) (Group Ⅰ: PVR≤3.5 wood's unit; Group Ⅱ: PVR 3.5～5 wood's unit; Group Ⅲ: PVR>5 wood's unit). RESULTS: TXB2/6-K-PGF1a and VWF:Ag increased significantly in PH, and varied in different PH groups (P<0.05). There was a positive association between PVR and VWF: Ag (r=0.89, P<0.05) and between pulmonary artery resistance (PAR)and VWF: Ag (r=0.82, P<0.05). The levels of tPA in PH did not differ from normal. With the progressive changes on the light microscopy, the increases in the volume density of rough endoplasmic recticulum and microfilament bundles on the transmission electron microscopy were more significant and the immunostain for VWF: Ag was more intense. CONCLUSIONS: The functional and structural changes of endothelial cells are important in the pathogenesis of PH secondary to CHD. The experiments provide a theoretical basis for the modification of PH secondary to CHD with drug therapy.
2001 Vol. 3 (03): 212-214 [
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WU Ben-Qing, XU Li-Ren, ZHONG Qiao
Characteristics of Micturition and Changes of Plasma and Urine Osmolarity in Neonates
OBJECTIVE: To assess micturition characteristics, bladder capacity and plasma and urine osmolarity in neonates. METHODS: Fortyfive neonates were divided into three groups according to post natal ages (Group A=～3 d, Group B=～7 d, Group C=>7 d). The time of micturition and voided volume were recorded. The residual urine volume was detected using a bladder volume instrument (BladderScan BVI 2500). Plasma and urine osmolarity were measured using the FM-6 pattern freezing point osmometer. RESULTS: ① There were significant differences (P<0.05) in the daynight voided volume and voided frequency between Group A and Groups B and C. ② The bladder emptying rates, maxium bladder capacity(MBC) and plasma urine osmolarity of Groups A, B and C were 0.80±0.07, 0.82±0.09, 0.79±0.10; 56±6, 65±14, 61±15 (ml); 285±5, 287±7, 286±8 (mOsm/L); 202±59, 185±65, 216±92 (mOsm/L) respectively and there was no significant difference between the 3 groups. ③ The multiple stepwise regression method was used to estabilish the best regression equation for predicting the MBC, which was 42±0.17X (ml) ［X=8 hours (10 Am～6 Pm) voided volume, F=15.561 (P<0.01)］. CONCLUSIONS: The bladder of normal neonates empties almost completely and its function is stable. There is no difference in the renal concentration function of neonates between day and night.
2001 Vol. 3 (03): 215-217 [
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LU Qin, LU Chang-Dong, REN Jian-Hua, WANG Jian, WANG Li-Jin, NI Ning, TANG Guang-Yu, FENG Ling-Ling
Changes of Nacety-β-D-AminoGlucosidase and Urinary Retinal Binding Protein in Newborns with Asphyxia Complicated by Renal Function Impairment
OBJECTIVE: To study the changes in Nacety-β-D-amino glucosidase (NAG) and urinary retinal binding protein (RBP) observed after renal function impairment in asphyxiated newborns. METHODS: Colorimetry of p nitrophoenol and ELISA were adopted to detect the activity of NAG and the content of RBP in asphyxiated full term and premature infants on days 1, 3 and 7, and in matched nonasphyxiated control infants. RESULTS: The levels of NAG and RBP were higher in both asphyxiated full term and premature infants than in the control group (P<0.01). The changes were most significant on day 3 after birth, and correlated with the degree of asphyxia. The degree and duration of renal dysfunction also correlated significantly with the degree of asphyxia. Urinary RBP levels in the asphyxiated newborns rose significantly within 1 day after birth compared to the controls (P<0.01), while there was no significant difference in the levels of NAG on day 1 of life in asphyxiated infants (P>0.05). CONCLUSIONS: NAG and RBP appear to be indexes of early diagnosis in renal dysfunction in asphyxiated infants, and RBP is a more sensitive one.
2001 Vol. 3 (03): 218-220 [
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LIU Xue-Mei, YU Jian, LUO Li-Man
Dynamic Variations of Serum Procalcitonin Levels in Neonates with Sepsis
OBJECTIVE: To study the changes of serum procalcitonin (PCT) in neonates with sepsis. METHODS: Serum PCT was determined using an immunoradiometric assay in 20 newborn infants with neonatal sepsis, 24 neonates with hypoxicischemic encephalopathy (HIE) and 16 healthy newborn controls. RESULTS: Increased levels of serum PCT were found in neonates with sepsis compared with the control group ［(112.23±10.13) μg/L vs (8.65±2.14) μg/L］, (P<0.01). After 1 week of treatment with appropriate antibiotics, PCT levels returned to normal. There was no difference between HIE neonates and the control group. CONCLUSIONS: Elevated serum PCT levels are noted in neonatal sepsis. PCT might be of value in the diagnosis of neonatal sepsis.
2001 Vol. 3 (03): 221-223 [
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224
XIA Li, JI Li-Xiao
Effect of Cytomegalovirus, Herpes Simplex Virus and Parvovirus B19 on Fetal Malformation
OBJECTIVE: To explore the relationship between cytomegalovirus (CMV), herpes simplex virus (HSV) and human parvovirus B19 (HPV-B19) and fetal malformation. METHODS: There were 16 infants with congenital malformation in the malformation group and 25 normal infants in the control group. CMV, HSV and HPV-B19 infections were diagnosed using the polymerase chain reaction technique (PCR) in the main organs and placentas of the 2 groups. RESULTS: The infection rate with CMV and HSV in the malformation group was 50.0% and 37.5% respectively, compared with the 8.0% and 8.0% respectively in the control group. (P<0.05). The HPV-B19 infection rate didn't differ in the 2 groups. The brain, liver and lungs were the organs most commonly involved in CMV infection, whereas in HSV infection the liver was most commonly involved, with HPV-B19 most commonly affecting the spleen. CONCLUSIONS: CMV and HSV infections are closely related to congenital malformation, but HPV B19 infection is not related to malformation.
2001 Vol. 3 (03): 224-226 [
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227
WU Jie, LANG Hui-Li, WEI Ke-Lun
Dynamic Changes in Renal Ca2+ATPase Activity after Acute Intrauterine Ischemia in Fetal Rats
OBJECTIVE: To study the dynamic changes in renal Ca2+ ATPase after acute intrauterine ischemia in fetal rats,and to explore the underlying mechanism of intracelluar Ca2+ overload secondary to perinatal asphyxia.METHODS: A rat model of acute intrauterine ischemia was developed using different degrees of clamping of the vesseles supplying blood to the uterus. A graded reperfusion model was made by reperfusing the uterine vessels for different lengths of time after clamping. The activity of Ca2+ATPase in cell membranes and mitochondrial fractions in fetal rat kidneys was assayed biochemically.RESULTS: Ca2+ATPase activity in cell membranes and mitochondria in the shamoperated group was (7.476±0.353) and (3.470±0.270) μmol.Pi/mgprot.h respectively. Activities in both fractions decreased after 15 minutes of ischemia ［(6.411±0.210) and (2.886±0.245) μmol.Pi/mgprot.h respectively; P<0.01］ and continued to decrease after 45 minutes ［(5.772±0.177) and (2.500±0.282) μmol.Pi/mgprot.h respectively; P<0.05］. During the reperfusion stage (after 15 minutes of ischemia), activities continued to decrease reaching a nadir after 8 hours ［(5.513±0.197) and (2.411±0.197) μmol.Pi/mgprot.h respectively］. There was significant difference between the 8hour reperfusion group and the 15-minute ischemia group (P<0.01). After 15 h of reperfusion enzyme activity levels began to increase. By 24 hours, the activity of Ca2+ATPase in cell membranes had increased to normal levels, but the actvity in mitochondria was still low compared with the 15-minute ischemia group ［(3.234±0.143) and (2.886±0.245) μmol.Pi/mgprot.h respectively; P<0.05］. CONCLUSIONS: Ca2+ATPase activity decreases in the fetal kidney after acute intrauterine ischemia. Dynamic changes in Ca2+ATPase activity may explain the intracellular Ca2+ overload leading to hypoxic ischemic renal injury.
2001 Vol. 3 (03): 227-231 [
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MA Li-Ya, CHANG Li-Wen, QUAN Yu-Feng
Effect of Hyperoxia on Nitric Oxide Synthase Expression in the Lung of Preterm Rats
OBJECTIVE: To investigate the role of endogenous nitric oxide (NO) in hyperoxiainduced lung injury, by studying the effect of hyperoxia on NO synthesis and nitric oxide synthase (NOS) expression in the lung of preterm rats. METHODS: Threedayold preterm rats were randomly assigned to a hyperoxia group (90% oxygen) and room air group. After 3 days or 7 days of exposure, the ratio of lung wet weight/dry weight (W/D), lung morphometry, NO content in bronchoalveolar lavage fluid (BALF), and the cellular distribution and expression of endothelial NOS (eNOS) and inducible NOS (iNOS) in the lung were measured. RESULTS: After 3 days of exposure, when compared with the control group, the hyperoxia group showed acute lung injury characterized by the presence of hyperaemia, red cell extravasation, and inflammatory infiltration. After 7 days of exposure, W/D was also increased in the hyperoxia group compared to the controls (5.54±0.41 VS 5.00±0.15, P<0.05), in addition to the pathologic changes. After 3 and 7 days of exposure, the NO content in BALF was significantly elevated in the hyperoxia group compared with that of the air group ［(17.06±5.86) μmol/L vs (5.59±2.03) μmol/L and (23.75±4.07) μmol/L vs (7.93±2.33) μmol/L, respectively, P<0.01］. In the lungs of the hyperoxia group, immunostaining for iNOS was observed in the airway and alveolar epithelium, and in inflammatory cells, to a greater degree than in the air group. The expression of iNOS in rats was stronger after 7 days of hyperoxic exposure than after 3 days. After 7 days of exposure, stronger immunostaining for eNOS in the airway epithelium of the hyperoxia group than of the air group was seen. CONCLUSIONS: Hyperoxia can significantly upregulate the expression of iNOS and eNOS in inflammatory cells and epithelium in the lungs of preterm rats and thus promote NO generation, suggesting that endogenous NO may mediate hyperoxiainduced pulmonary injury.
2001 Vol. 3 (03): 232-235 [
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236
ZHU Chang-Lian, WANG Xiao-Yang
Effect of MK-801 on Caspase-3 Activation and Apoptosis after Cerebral Hypoxic Ischemic Injury in Neonatal Rats
OBJECTIVE: To study the effect of MK-801, the antagonist of NMDA receptor, on Caspase-3 activation and apoptosis after cerebral hypoxic ischemic injury in neonatal rats. METHODS: Sevendayold rat pups were injected with either 0.5 mg/kg MK-801 or normal saline immediately after cerebral hypoxicischemic injury (HI). The pups were killed 24 h after the injection. Brain damage was evaluated using MAP-2 immunostaining. Caspase-3 activation was examined using antip17 subunit antibody. Apoptosis was examined by in situ labeling of hairpin probe (HPP). The infarction area was calculated according to MAP-2 staining. Active Caspase-3 cells and HPP positive cells were counted in the MAP-2 negative area of the cortex. RESULTS: The infarction area was reduced from (52±12)% to (23±5)% after the treatment with MK-801. A few Caspase-3 and HPP positive cells were also found in the cortex of normal rat brains. The number of positive cells reached a peak at 24 h after HI. Both the number of Caspase-3 (from 65±8 to 40±6.7 per high power visual field) and HPP (from 61.6±11.5 to 12.6±5.2 per high power visual field) positive cells were decreased after the treatment with MK-801. CONCLUSIONS: MK-801 inhibits Caspase-3 activation and reduces the number of apoptotic cells. NMDA is involved in the activation of the apoptotic process in the immature brain after HI.
2001 Vol. 3 (03): 236-238 [
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JIA Yan-Jie, HU Hai-Tao, REN Hui-Min, WANG Wei-Xi
Apoptosis in Cultured Rat Islets Cells
OBJECTIVE: To investigate the mechanism of apoptosis in cultured rat islets. METHODS: Rat islets were sampled for isolation, 1, 3, 7, 14, 21 day (s) in culture. The distribution of reticulin for peri insular basement membrane was observed and stained by Foot's assay. Apoptotic cells were assessed by electron microscopy and fluorescent microscopy with Hoechst 33258 staining. The islet viability was determined by MTT colorimetric assay indirectly. The positive rate of islet Fas and Fas ligand (Fas L) protein expression was measured by immunocytochemistry ABC staining. RESULTS: Immediately after islet isolation, the reticulin periinsular basement membrane was absent. Typical morphological characteristics of islet apoptosis were observed, such as nuclear shrinkage and migration of chromatin towards the nuclear membrane, after 3 d of culture. The apoptotic rate was (7.6±5.8)% after 3 d, and increased to (63.0±2.6)% and (47.2±8.1)% after 14 d and 21 d of culture. Catenin β expression diminished after 7 d of culture by immunocytochemistry ABC staining. The positive rate of islet Fas protein expression increased from (8.1±1.8) after 3 d to (38.5±4.7)% after 14 d, and (35.6±6.5)% after 21 d of culture. Although the expression of Fas-L protein was negative before days 3, the positive rate of Fas-L protein was (6.8±3.2)% after 7 d, and was upregulated significantly after 14 d and 21 d of culture to (19.6±4.8)% and (12.4±7.1)% respectively. Moreover, cumulative quantities of insulin production and islet viability were reduced significantly. CONCLUSIONS: Rat islets are primed to undergo apoptosis in culture, and this event involves some association between cell-surface Fas and its ligand.
2001 Vol. 3 (03): 239-242 [
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243
LIU Jian-Ping, ZHANG Guo-Cheng, XU Dong-Liang
Calmodulin in Brain Tissues of Newborn Rats with Hypoxic Ischemic Encephalopathy and the Therapeutic Effect of Cerebrolysin
OBJECTIVE: To study the relationship between calmodulin (CaM) concentrations in brain tissues of newborn rats and the pathogenesis of hypoxicischemic encephalopathy (HIE) and the therapeutic effect of cerebrolysin on HIE. METHODS: Morphological and metabolic effects of cerebral ischemia were investigated in a newborn rat model of neonatal HIE. In addition, the effect of cerebrolysin (EBEWE, Austria) on acute brain hypoxicischemia was studied. Eighty eight HIE newborn rats (48 cerebrolysintreated and 40 controls) were subjected to left cerebral artery ligation and one hour later they were placed in a hypoxic environment (8% oxygen and 92% nitrogen mixed) for two hours. Immediately afterwards, cerebrolysin (2.5 ml/kg body mass) was administered daily, either by intracerebroventricular infusion (i.c.v) or by intramuscular injection. CaM concentrations were determined using cyclicnucleotide dependent phosphodiesterase methods, and 15 normal rats were used as the control group. RESULTS: CaM concentrations in brain tissues increased in HIE group at different duration after hypoxicischemia: 24 h=(521.27±46.04) μg/g brain tissue; 48 h=(509.52±35.98) μg/g brain tissue; 72 h=(421.05±31.81) μg/g brain tissue, compared to the control group ［(187.63±54.22) μg/g brain tissue］ (P<0.01). Cerebrolysin treatment groups facilitated recovery, as indicated by a decrease in CaM concentration (i.c.v group: 24 h=(435.21±56.17) μg/g brain tissue; 48 h=(260.38±32.43) μg/g brain tissue; 72 h=(197.64±19.21) μg/g brain tissue; i.m.group: 24 h=(441.04±30.66) μg/g brain tissue; 48 h=(305.39±32.99) μg/g brain tissue; 72 h=(217.71±52.89) μg/g brain tissue (P<0.01). Morphological examination showed that the volume of neuron degeneration, necrosis and intercellular edema was increased in HIE groups and significantly reduced in cerebrolysin treatment groups. CONCLUSIONS: CaM concentration in brain tissues is increased after hypoxicischemic injury and may be implicated in the pathogenesis of newborn rats with HIE. Cerebrolysin is effective in protecting brain cells from further damage through its influence on cerebral CaM concentration.
2001 Vol. 3 (03): 243-245 [
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247
XU Jian-Wen, ZHANG Geng, WANG Wei, KANG Zhong-Han
Effect of Hypoxia on the Activity of the Tissue-Type Plasminogen Activator of Brain Microvascular Endothelial Cells in Mice
OBJECTIVE: To observe the effect of hypoxia on the activity of the tissue-type plasminogen activator (TPA) of brain microvascular endothelial cells in mice. METHODS: Microvascular endothelial cells obtained from the brains of newborn mice were cultured in primary and passage under room air and hypoxic conditions to observe the variation in TPA activities. TPA activity was assayed using the enzyme linked immunosorbent assay (ELISA) technique. RESULTS: Following hypoxia, TPA activity in endothelial cells increased in primary culture compared with the controls: (22.5±1.5)×10-2 IU/ml vs (19.4±1.7)×10-2 IU/ml (P<0.01). TPA activity following hypoxia was not altered in passaged cells. CONCLUSIONS: Neuronal damage following hypoxia may be associated with increased endothelial TPA activity.
2001 Vol. 3 (03): 247-249 [
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HONG Yan, LIU Jun-Yan, ZHANG Yu-Hou, WANG Hong-Ling, DENG Zi-Niu
Effect of Angelica Polysaccharide on the Promotion of Thymocyte Proliferation and RBC C3bR Immune Adhesion Function in Radiated Mice
OBJECTIVE: To explore the effect of angelica polysaccharide (AP) on the thymocyte proliferation and RBC C3bR immune adhesion function in radiated mice. METHODS: Mice were divided into control, irradiated and AP treated also irradiated groups (10 per group). The proliferation of thymocytes and RBC C3bR rosette formation rate were then examined on day 10 after AP was given. RESULTS: The thymocyte proliferation responses (0.24±0.01) and the RBC C3bR rosette formation rate ［(16.46±0.76)%］ of the irradiated control group were significantly lower than those of the non irradiated controls ［(0.35±0.01) and (22.30±1.26)%, respectively; P<0.01］］. The thymocyte proliferation (0.31±0.01) and RBC 36bR rosette formation ［(21.07±1.22)%］ in the treatment group were significantly higher (P<0.01) than those of the irradiated control group, and approached nonirradiated control levels. CONCLUSIONS: AP can reverse radiationinduced reduction in the proliferation response of thymocytes and in RBC immune adhesion function.
2001 Vol. 3 (03): 250-252 [
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WANG Lai-Shuan, ZHU Chang-Lian, CHENG Xiu-Yong, WANG Xiao-Yang, YANG Jing-Li, WEN Hong-Tao, ZHU Hong, ZHOU Yuan-Cong
Ameliorative Effect of the Nerve Growth Factor
OBJECTIVE: To explore the mechanism of ameliorative effects of the nerve growth factor (NGF) on newborn rats with hypoxic ischemic brain damage (HIBD). METHODS: NGF dosage (0.1 μg/kg and 0.3 μg/kg) was administrated intraperitoneally to the rat pups 30 minutes before and immediately following the hypoxic exposure. The percentage of brain water content was determined according to the formula: (wet weight dry weight)/wet weight×100=% water content. Brain tissue calcium concentration was measured with atomic absorption spectroscopy. Monoamine neurotransmitter concentration in the rat brain was measured using high performance liquid chromatography with electrochemical detector (HPLCECD). RESULTS: Brain water content, calcium conentration and monoamine neurotransmitters (except DOPAC) concentration increased significantly after hypoxicischemic insult in the ipsilateral hemisphere of the HIBD group compared with the contralateral hemisphere and/or control group: water contents=(91.44±2.21)% vs (82.99±6.53)% (P<0.05) and (91.44±2.21)% vs (87.22±1.13)% (P<0.05) respectively; calcium concentration=(255.24±122.6) μmol/g dry weight vs (44.12±13.54) μmol/g dry weight (P<0.01) and (255.24±122.6) μmol/g dry weight vs (42.68±4.35) μmol/g dry weight (P<0.01), respectively. There were significant discreases in water content, calcium concentration and monoamine neurotransmitters (except DOPAC) concentration in the 0.1 μg/kg and 0.3 μg/kg NGFtreated HIBD group (compared with untreated HIBD group): water contents=(86.86±0.86)% and (87.45±0.3)%, respectively; calcium concentration=(44.34±3.10) μmol/g dry weight and (42.03±14.99) μmol/g dry weight, respectively. No differene was found between the NGF group and control group. CONCLUSIONS: Water content, calcium concentration and monoamine neurotransmitters may be involved, alone or interactively, in the pathophysiologic process of HIBD. NGF has ameliorative effects on newborn rats with HIBD, possibly through maintaining calcium homeostasis, ameliorating brain edema and attenuating or inhibiting the neurotoxic effects of monoamine neurotransmitters.
2001 Vol. 3 (03): 253-256 [
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LIU Xiao-Yan, DANG Xi-Qiang, WAN Wu-Qing, WEN Chuan, YANG Hui-Cai
Logistic Regression Analysis of Nosocomial Infection Risk Factors in Acute Leukemia in Children
OBJECTIVE: To investigate nosocomial infection risk factors in acute leukemia in children in order that they are wellrecognized by clinic doctors and the mortality rate associated with them will be decreased. METHODS: A retrospective review of the medical records of 306 children with acute leukemia admitted between January, 1995 and December, 2000 was performed. The data were analyzed with the single factor χ2 test, t test and multifactor logisticregression analysis. RESULTS: The incidence of nosocomial infection was 28.43%(87/306), and consisted mainly of respiratory tract and oral infections. The length of hospitalization, white blood cell count, neutrophil and platelet count were found to be independent risk factors for nosocomial infection. CONCLUSIONS: The length of hospitalization, reduced white blood cell count and neutrophil and platelet count are independent risk factors for nosocomial infection in children with leukecnia.
2001 Vol. 3 (03): 257-259 [
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ZHANG Yan-Min, LEI Chun-Lian, CHENG Ge-Sheng, YANG Yu-Cong
Expression of CD45RA+ and CD45RO+ in PBMCs of Infants with RSV LRTIs
OBJECTIVE: To study the expression of CD4+ (helper T lymphocyte), CD45RA+ and CD45RO+ in PBMCs of infants with RSV LRTIs. METHODS: CD4+ and CD45RA+ were detected in 30 patients with RSV LRTIs and CD45RO+ in PBMCs was also assayed except for CD+4 and CD45RO+ in 11 of them with the flow cytometric analysis. Nine healthy children were used as the controls. RESULTS: In RSV infants, CD4+ cells ［(32.74±10.60)%］ decreased and CD45RA+ ［(80.65±10.35)%］ increased compared with the controls ［(40.76±6.82)% and (67.34±7.54)%, respectively］ (P<0.01). CD45RO+ cells also decreased, but not significantly. CONCLUSIONS: There is a disorder of immune function in PBMCs in infants with RSV.
2001 Vol. 3 (03): 260-261 [
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XIE Qiang-Ling, WEN Zhi-Hong, HONG Mei, HUANG Xiao-Fang, NONG Li-Ping, MA Jin-Zhong, ZHANG Xin, HUANG Qian-Li
Determination and Significance of Granulocyte Colony Stimulating Factors in Children with Respiratory Airway Infection
OBJECTIVE: To explore changes of the serum granulocyte colony-stimulating factor (G-CSF) in children with acute respiratory airway infection. METHODS: Venous blood was obtained from 30 normal children, 189 children with acute respiratory airway infection without treatment and 50 children (from the group of 189 children) treated with antibiotics for 5～7 days. G-CSF levels were determined using the double antibody sandwich enzymelinked immunosorbent assay (PAS ELISA) and C reaction protein (CRP) levels were assayed using singleradial immunodiffusion. RESULTS: Of the 189 patients with acute respiratory airway infection, 105 were positive for G-CSF and 68 were positive for CRP (>12 ug/ml). Both G-CSF and CRP were positive in 23.8% of the patients. After a 5～7 day treatment course, the CRP of 50 turned from positive to negative and the G-CSF of 16 remained positive. CONCLUSIONS: Serum G-CSF levels increase in the stage of acute infection.
2001 Vol. 3 (03): 262-264 [
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LIU Jian-Xin, WANG Ming-An, HUANG Bei, HONG Chao, JIN Long-Yu, WANG Ying, LIU Xiao-Wu
Cardiopulmonary Bypass with the Bloodless Priming Technique in Open heart Surgery in Children
OBJECTIVE: To explore the effect of cardiopumlonary bypass using the bloodless priming technique in open-heart surgery in children. METHODS: Fifty cases of atrial septal defect (ASD) or ventricular atrial septal (VSD) were randomly divided into a bloodless priming group (n=25) and a control group receiving blood priming (n=25). The Hct, furosemide dosage, urine output, pericardial and mediastinal drainage, and time of wakening and of mechanical ventilatory support were deternined intraoperatively and postoperatively. RESULTS: The intra and 12 h post operative Hct ［(19.0±6.8) % and (30.4±25.2)%, respectively］ in the bloodless group was lower than that in the blood primed group ［(23.4±10.6)% and (33.2±23.4)%, respectively］ (P<0.05). The intra and post operative doses of furosemide ［(5.2±0.8) mg and (4.5±0.6) mg, respectively］ in the bloodless group were larger than those in the blood primed group ［(1.2±1.0) mg and (1.5±0.5) mg, respectively］ (P<0.05). The intra and 24 h post operative urine output in the blood less group ［(218±56) ml and (278±38) ml, respectively］ was higher than that in the bloodprimed control group ［(78±36) ml and (189±62) ml, respectively］. The Hct did not differ between the bloodless group and bloodprimed group 24 h after the operation ［(38.6±25.2) % vs (38.8±24.3) %］. Pericardial and mediastinal drainage and time of wakening and postoperative mechanical ventilatory support did not differ in the two groups. CONCLUSIONS: Cardiopulmonary bypass using the bloodless priming tecnique appears to be safe and practicable in pediatric openheart surgery procedures.
2001 Vol. 3 (03): 265-267 [
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MA Geng, GE Zheng, WANG Min-Biao
Improved small transverse incision in the treatment of cryptorchidism in children
No abstract available
2001 Vol. 3 (03): 268-269 [
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3377
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1KB] [
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68KB] (
1374
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330
CHEN Ying-Cai, SI Kai-Ying, GAO Wei-Juan, WANG Cheng, JI Shu-Ping, HAO Yue, HE Xin, CHANG Bao-Zhong, DONG Hui-Ping
Clinical application of monitoring serum VPA in children with epilepsy
No abstract available
2001 Vol. 3 (03): 330-332 [
Abstract
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3955
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1KB] [
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81KB] (
1519
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333
WANG Yu-Yuan, HUANG Zhong-Yan, OU-YANG Yan-Fang
Therapeutic effect of mezolin combined with oryzanol on child anorexia pollakiuria
No abstract available
2001 Vol. 3 (03): 333-334 [
Abstract
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3878
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1KB] [
PDF
67KB] (
1212
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CLINICAL RESEARCH
270
LI Shuang-Shuang, ZHANG Rui-Xuan, DU Wei-Xing, SHI Wei-Ping
Dynamic variations of plasma growth hormone levels in neonates with hypoxic-ischemic encephalopathy
No abstract available
2001 Vol. 3 (03): 270-272 [
Abstract
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3372
) [
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1KB] [
PDF
76KB] (
962
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273
WANG Xiu-Juan, LIU Jian-Jun, YIN Li, WANG Xiu-Mei, LANG Zhuo-Ying, PANG Zhi-Zhen
Therapeutic effect of hyperbaric oxygen in neonatal hypoxic-ischemic encephalopathy
No abstract available
2001 Vol. 3 (03): 273-274 [
Abstract
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3521
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1KB] [
PDF
71KB] (
1146
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275
SONG Xiong, YUE Peng-Ling
Electroencephalography in the diagnosis and prognosis of neonatal hypoxic-ischemic encephalopathy
No abstract available
2001 Vol. 3 (03): 275-276 [
Abstract
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3705
) [
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1KB] [
PDF
68KB] (
1478
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277
YANG Gui-Rong, WU Yan-Bin, LIAN Li-Min
Follow-up CT survey of neonatal hypoxic-ischemic encephalopathy
No abstract available
2001 Vol. 3 (03): 277-278 [
Abstract
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2921
) [
HTML
1KB] [
PDF
68KB] (
1026
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279
HUANG Hui-Wen, FENG Ze-Kang, XIAO Gang-Ming, ZHU Xiao-Yu
Levels of serum free fatty acids in neonatal asphyxial and hypoxic-ischemic encephalopathy
No abstract available
2001 Vol. 3 (03): 279-280 [
Abstract
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3131
) [
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1KB] [
PDF
70KB] (
955
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281
LI Zhan-Kui, BU Er-Jia, LIU Ya, LI Rui-Lin, ZHOU Jian-Ping, LI Jing
Therapeutic effect of ceftriaxone on bacterial infectious diseases in neonates
No abstract available
2001 Vol. 3 (03): 281-282 [
Abstract
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3513
) [
HTML
1KB] [
PDF
68KB] (
1231
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283
ZHANG Xiao-Yan, TAN Zun-Ke, WANG Huan-Xiu
Sepsis caused by omphalitis in neonates
No abstract available
2001 Vol. 3 (03): 283-284 [
Abstract
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3413
) [
HTML
1KB] [
PDF
70KB] (
1095
)
285
ZHOU Wei, ZHANG Xiu-Fang, LIANG Hong, ZHANG Hong
Characteristics and treatment of pneumothorax in mechanically ventilated neonates
No abstract available
2001 Vol. 3 (03): 285-286 [
Abstract
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3045
) [
HTML
1KB] [
PDF
77KB] (
1266
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287
LU Hui, WANG Yu, XUE Xin-Dong, WEI Ke-Lun
Risk factors for pulmonary air leak in neonatal hyaline membrane disease
No abstract available
2001 Vol. 3 (03): 287-288 [
Abstract
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3210
) [
HTML
1KB] [
PDF
72KB] (
964
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289
WANG Juan
Pulmonary air leak caused by mechanical ventilation in neonates
No abstract available
2001 Vol. 3 (03): 289-290 [
Abstract
] (
2909
) [
HTML
1KB] [
PDF
71KB] (
1059
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291
LI Jian, WU Yu-Qun, ZHAO Zhe, CHENG Bing-Mei
Influence of perinatal factors on β2-MG levels in neonates
No abstract available
2001 Vol. 3 (03): 291-292 [
Abstract
] (
3160
) [
HTML
1KB] [
PDF
70KB] (
994
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293
YAO Cong-Yue, DENG Xiao-Yi
Changes in the etiology of unconjugated hyperbilirubinemia in neonates after the introduction of mother-infant rooming-in
No abstract available
2001 Vol. 3 (03): 293-294 [
Abstract
] (
3152
) [
HTML
1KB] [
PDF
70KB] (
1006
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295
CHEN Bi-Qin, PENG Qiao-Di, ZHOU Kang, ZHU Mei-Lan
Neonatal behavioral neurological assessment and brainstem auditory evoked potentials in neonatal hyperbilirubinemia
No abstract available
2001 Vol. 3 (03): 295-296 [
Abstract
] (
3828
) [
HTML
1KB] [
PDF
71KB] (
1220
)
297
ZHANG Lan-Ying, XU Lu-Jun, DENG Hong-Zhen, NIE Li-Hong, JIN Ning, DU Hui-Jie
Correlation between bone alkaline phosphatase activation in neonates and pregnant mothers
No abstract available
2001 Vol. 3 (03): 297-298 [
Abstract
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4062
) [
HTML
1KB] [
PDF
75KB] (
967
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299
LI Hei-Da, DENG Guo-Quan, HUANG Xin-Hua, HU Chun-Yu
Levels of cerebrospinal fluid β-endorphin in premature infants with primary apnea
No abstract available
2001 Vol. 3 (03): 299-300 [
Abstract
] (
3286
) [
HTML
1KB] [
PDF
69KB] (
974
)
301
YU Hong-Ling
Dynamic variations of serum bilirubin in neonates with asphyxia
No abstract available
2001 Vol. 3 (03): 301-302 [
Abstract
] (
3074
) [
HTML
1KB] [
PDF
66KB] (
935
)
303
SHI Yu
Diagnosis and treatmet of cardiac rhythm disorders in newborn infants
No abstract available
2001 Vol. 3 (03): 303-304 [
Abstract
] (
3111
) [
HTML
1KB] [
PDF
69KB] (
1306
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305
ZHENG Ming-Ci, QUAN Yu-Feng, CHENG Zhen-Yu, WANG Yan, ZHANG Guo-Feng
Vitamin E concentrations in the blood, cord blood and breast-milk
No abstract available
2001 Vol. 3 (03): 305-306 [
Abstract
] (
3203
) [
HTML
1KB] [
PDF
69KB] (
981
)
307
CHEN Ping-Yang, XIE Zong-De, WU Yong
Levels of IL-6 and TNF-α in mononuclear cells of fresh breast milk and refrigerated breast milk
No abstract available
2001 Vol. 3 (03): 307-308 [
Abstract
] (
3111
) [
HTML
1KB] [
PDF
69KB] (
972
)
309
JIN Yan-Liang, YU Tao, RUAN Yi, LIU Han-Sao, CAO Lan-Fang
Detectio of eosinophil cationic protein in children with mycoplasma pneumonia
No abstract available
2001 Vol. 3 (03): 309-310 [
Abstract
] (
3406
) [
HTML
1KB] [
PDF
70KB] (
1141
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311
HE Rong-Li, ZHENG Miao
Changes in blood sugar and the thyroid function of neonates with asphyxia
No abstract available
2001 Vol. 3 (03): 311-311 [
Abstract
] (
3208
) [
HTML
1KB] [
PDF
78KB] (
1035
)
312
HAN Zai-De, WANG Run-Wen, WANG Hui, PENG Ren-Luo
CT characteristics of tuberculosis meningitis in children
No abstract available
2001 Vol. 3 (03): 312-314 [
Abstract
] (
3269
) [
HTML
1KB] [
PDF
92KB] (
1013
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315
JIN Yu, LI Yu-Ning, DUAN Hong-Mei, WU Ying
Pathogenesis of acute respiratory tract infections in children in the Lanzhou Region
No abstract available
2001 Vol. 3 (03): 315-316 [
Abstract
] (
3421
) [
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1KB] [
PDF
68KB] (
1076
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317
PAN Wei, XU Jin-Sheng
Prevention of renal lesions with heparin in Henoch-Schonlein purpura
No abstract available
2001 Vol. 3 (03): 317-318 [
Abstract
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3256
) [
HTML
1KB] [
PDF
70KB] (
955
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319
SONG Chao-Zheng, ZENG Hua-Song, TAN An-Ping
Renal tubular functions testing in children with glomerulopathy
No abstract available
2001 Vol. 3 (03): 319-320 [
Abstract
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3355
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1KB] [
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70KB] (
1012
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321
XIAO Xue-Zhou
Scrub typhus in children(68 cases)
No abstract available
2001 Vol. 3 (03): 321-323 [
Abstract
] (
3302
) [
HTML
1KB] [
PDF
70KB] (
1054
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323
HU Yi-Zhi
Effect of naloxone as an auxiliary therapy on asthmatic diseases in children
No abstract available
2001 Vol. 3 (03): 323-324 [
Abstract
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3285
) [
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1KB] [
PDF
0KB] (
499
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325
GAO Bing-Jie, SUN Zhi-Rong, ZHANG Yu-Qin, ZHAO Jiang-Yong, CHEN Rui
Relationship between asthma and mycoplasma pneumonia in children
No abstract available
2001 Vol. 3 (03): 325-325 [
Abstract
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3401
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1KB] [
PDF
70KB] (
1165
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326
LI Rui, DENG Xing-Qiang, LIU Xiao-Ming, LIANG Li-Jun
Effect of loading doses of carbamazepine in the treatment of status epilepticus and continuous seizures
No abstract available
2001 Vol. 3 (03): 326-327 [
Abstract
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3431
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1KB] [
PDF
70KB] (
1123
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328
ZHAO Gui-Jie, ZHOU Jian-Qin
Clinical characteristics of abdominal anaphylactoid purpura
No abstract available
2001 Vol. 3 (03): 328-329 [
Abstract
] (
3411
) [
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1KB] [
PDF
67KB] (
1476
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CLINICAL EXPERIENCE
335
HEI Ming-Yan
Rodent HIE models in medical experiments
No abstract available
2001 Vol. 3 (03): 335-335 [
Abstract
] (
2845
) [
HTML
1KB] [
PDF
67KB] (
910
)
REVIEW
337
XIAO Yan-Yan
Application of the three-dimensional echocardiography in congenital heart diseases
No abstract available
2001 Vol. 3 (03): 337-340 [
Abstract
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2628
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1KB] [
PDF
87KB] (
1122
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CJCP
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Copyright © 2004-2005 Chinese Journal Of Contemporary Pediatrics All rights reserved.
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Tel: 0731-84327402 Fax: 0731-84327922 E-mail: cjcp1999@csu.edu.cn
