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2010 Vol.  12 No.  08
Published: 2010-08-15
EXPERT LECTURE
CLINICAL RESEARCH
EXPERIMENTAL RESEARCH
CLINICAL EXPERIENCE
CASE REPORT
REVIEW
EXPERT LECTURE
597 Koravangattu SANKARAN
Hypothermic neuroprotection in neonates-cooler head prevails

No abstract available
2010 Vol. 12 (08): 597-601 [Abstract] ( 4788 ) [HTML 1KB] [PDF 1050KB] ( 1790 )
CLINICAL RESEARCH
602 MAI Hui-Rong, LI Chang-Gang, WANG Ying, SHI Hong-Song, ZHAO Wei-Ling, CHEN Yun-Sheng, CHEN Xiao-Wen
Status of iron metabolism and erythropoietic proliferation in children with various genotypes of thalassemia
OBJECTIVE: To study the status of iron metabolism and erythropoietic proliferation in children with various genotypes of thalassemia. METHODS: Serum concentrations of ferritin (SF), transferrin receptor (sTfR) and erythropoietin (EPO) were measured in 158 children with thalassemia. The differences in the concentrations of the three indices among children with different genotypes of thalassemia were compared. The correlations of the hemoglobin level with sereum SF, sTfR and EPO levels were assessed. RESULTS: Among the 158 children with thalassemia, 52(32.9%) were diagnosed with alpha-thalassemia minor, 27(17.1%) with HbH disease, 59(37.4%) with beta-thalassemia minor, 13(8.2%) with beta-thalassemia major, and 7(4.4%) with combining alpha beta thalassemia. The SF levels in children with HbH disease or beta-thalassemia major were significantly higher than those in the other thalassemia groups (P<0.01). The sTfR levels in children with beta-thalassemia major were the highest when compared with those in the other thalassemia groups (P<0.05). The EPO levels in children with beta-thalassemia major were also the highest when compared with those in the other thalassemia groups (P<0.01). There was a negative correlation between hemoglobin and EPO levels in children with HbH disease (r=-0.656, P<0.01) and beta-thalassemia major (r=-0.641; P<0.05). CONCLUSIONS: The status of iron metabolism and erythropoietic proliferation is different in children with different genotypes of thalassemia. A combined measurement of SF, sTfR and EPO may reflect the status of erythropoietic proliferation.［Chin J Contemp Pediatr, 2010, 12 (8):602-604］
2010 Vol. 12 (08): 602-604 [Abstract] ( 6975 ) [HTML 1KB] [PDF 963KB] ( 2068 )
605 XIONG Hao, ZHANG Yao-Dong, HU Qun, SUN Yan, LIU Shuang-You, ZHANG Liu-Qing, LIU Ai-Guo, WANG Guan-Ling
Biological characteristics of T-lineage acute lymphoblastic leukemia in 23 children
OBJECTIVE: To investigate the biological characteristics of childhood T-lineage acute lymphoblastic leukemia (T-ALL) and their clinical significance. METHODS: Immunophenotyping was performed by three-color flow cytometry analysis using CD45 /SSC gating in 23 children with newly diagnosed T-ALL. Meanwhile cytogenetic analysis was performed. RESULTS: CD3+ expression of T-lineage antigens was apparently higher than CD7+ and CD5+ expression. CD19+ expression of B-lineage antigens was apparently higher than CD22+, CD10+ and CD20+ expression. Myeloid antigen was expressed in 4 cases (17%). CD34+ and HLA-DR+ were observed in 4 cases (17%) and 5 cases (22%), respectively. cCD3+ and cCD79+ were expressed in 23 cases (100%) and 22 cases (96%), respectively. The chromosome detection in 8 cases with T-ALL showed hyperdiploid or Ph+ chromosome (one case each). The fusion gene detection in 5 cases showed MLL rearrangements in two cases and positive SIL/TAL1 fusion gene in one case. CD3 expression was related with the complete remission rate. CONCLUSIONS: Immunophenotyping is an important tool for diagnosis of T-ALL. However, the immunophenotype of T-ALL is heterogeneous. So, immunophenotyping along with cytogenetic and molecular genetic analysis is needed in the treatment and prognosis evaluation of T-ALL.［Chin J Contemp Pediatr, 2010, 12 (8):605-608］
2010 Vol. 12 (08): 605-608 [Abstract] ( 7472 ) [HTML 1KB] [PDF 976KB] ( 2406 )
609 DUAN Yan-Long, WU Run-Hui, YANG Shuang, ZHANG Rui, ZHANG Li, ZHOU Xuan, JIANG Jin, JIN Mei
Clinical features of hepatitis-associated aplastic anemia in children
OBJECTIVE: To study the clinical features of hepatitis-associated aplastic anemia (HAAA) in children. METHODS: The clinical data of the children with newly diagnosed HAAA from January 2007 to December 2008 were respectively studied, including clinical manifestations, and blood routine, bone marrow examination, viral serology and immune function results as well as treatment and prognosis. RESULTS: A total of 8 children were confirmed as HAAA, accounting for 4.9% in children with aplastic anemia. There were 7 males and 1 female. The median age was 7.5 years (range 4.4 to 10.3 years) at diagnosis. They had negative serologic results and the causes of hepatitis could not be identified. The median interval from hepatitis occurrence to blood cell reduction was 6 weeks. Three cases were diagnosed as severe aplastic anemia and 5 cases as very severe aplastic anemia. Severe T cell immune disorders were found in all 8 cases. The percentage of Ts cells increased and the percentage of Th cells decreased significantly in the 8 children with HAAA. Four children survived after immune suppress treatment, three children died within one month after diagnosis and one child required own discharge without treatment. CONCLUSIONS: HAAA is more frequent in male school children. The children with HAAA have severe T cell immune disorders, with a higher early death rate. Immune suppress treatment is effective.［Chin J Contemp Pediatr, 2010, 12 (8):609-612］
2010 Vol. 12 (08): 609-612 [Abstract] ( 6201 ) [HTML 1KB] [PDF 972KB] ( 2005 )
613 YU Tao, BAO Lian-Sheng, YANG Lao-Rong, LIU Fang
Antibiotic resistance of Acinetobacter Baumanii isolated from children in Wuhan between 2006 and 2008
OBJECTIVE: To investigate the variance of antibiotic resistance of Acinetobacter Baumanii isolated from children in Wuhan between 2006 and 2008. METHODS: Bacterial susceptibility testing was carried out by the Kirby-Bauer method in 679 strains of Acinetobacter Baumanii isolated in Wuhan Children’s Hospital between 2006 and 2008. The results were assessed according to the guidelines of the Clinical and Laboratory Standards Institute (2008). RESULTS: The nonsusceptible rates of Acinetobacter Baumanii to ceftazidime, cefepime and piperacillin/sulbactam increased significantly in 2007 compared with those in 2006 (P<0.05). By comparing the results of 2007, it was suggested that the nonsusceptible rates of Acinetobacter Baumanii to ceftazidime, cefepime, piperacillin/sulbactam, cefoperazone/sulbactam, imipenem and meropenem increased significantly in 2008 (P<0.05). CONCLUSIONS: The nonsusceptible rates of Acinetobacter Baumanii to β-lactam antibiotics in children from Wuhan increased significantly year by year between 2006 and 2008.［Chin J Contemp Pediatr, 2010, 12 (8):613-615］
2010 Vol. 12 (08): 613-615 [Abstract] ( 5657 ) [HTML 1KB] [PDF 960KB] ( 1749 )
616 SHA Kan, XU Pei-Ru, DUO Li-Kun, GU Li-Ba-Ha, GAO Xiao-Mei, JI Ping
Prevalence of upper respiratory tract group A Streptococcus carriage in school-age children from Tulufan City and Buerjin County of Xinjiang Province
OBJECTIVE: To study the prevalence rate of upper respiratory tract group A Streptococcus (GAS) carriage in school-age children from Xinjiang Province. METHODS: A total of 478 children at age of 9-12 years from Tulufan City and Buerjin County of Xinjiang Province were enrolled by random cluster sampling. Throat swab cultures were performed once each season for the determination of presence of GAS. RESULTS: In the 1 827 samples, 196 GAS strains were isolated, with a GAS carrier rate of 10.7%. The prevalence rate of GAS carrier in Tulufan City ranged from 3.7%-16.5% compared with 4.7%-21.4% in Buerjin County (P<0.05). The prevalence rate of GAS carrier in winter is the highest, followed by in autumn, spring and summer in both regions. There were significant differences in the GAS carriage rate in autumn between the two regions. There were no significant differences in the GAS carriage rate between boys and girls. Of the 196 GAS strains, 133 from Han, 22 from Uygur and 41 from Hazakh children. There were significant differences in the prevalence rate of GAS carriage among children with different ethic groups. CONCLUSIONS: The prevalence rate of GAS carriage is high in school-age children from Tulufan and Buerjin of Xinjiang Province. The GAS carrier rate is associated with the season and ethic group. The children from Buerjin County present a higher GAS carrier rate than those from Tulufan City.［Chin J Contemp Pediatr, 2010, 12 (8):616-618］
2010 Vol. 12 (08): 616-618 [Abstract] ( 5908 ) [HTML 1KB] [PDF 956KB] ( 1419 )
619 HEI Ming-Yan, ZHAO Ling-Ling, WU Zhi-Xiang, TIAN Lang, TAN Yan-Juan
Umbilical venous catheterization related infection in the neonatal intensive care unit
OBJECTIVE: To study the incidence and pathogens of umbilical venous catheterization (UVC) related infection in the neonatal intensive care unit (NICU). METHODS: A total of 112 neonates (birth weight 1 500 g) who received UVC within 24 hrs after birth were included. Blood culture was performed right after UVC. At 24 hrs and 1 week after UVC, umbilical skin scrub cultures were performed. Skin redness and swelling for more than 24 h, or severe abdominal distension, or poor general condition for unknown reason after UVC, or positive blood culture results, were the criteria for catheterization related infection. RESULTS: The incidence rate for UVC related infection was 8.9%. Total culture positive rate was 9.4%. At 24 hrs and 1 week after UVC, the umbilical skin scrub culture positive rate was 7.1% and 16.2%, respectively. Rate of Gram positive and Gram negative pathogens was 55.2% and 44.8%, respectively. Group B Streptococcus was main Gram positive pathogen. Klebsiella and E.coli were the main Gram negative pathogens. CONCLUSIONS: UVC is, to some extent, related to nosocomial infection in the NICU. Among UVC related infection, Gram positive and Gram negative pathogens take almost the chance.［Chin J Contemp Pediatr, 2010, 12 (8):619-621］
2010 Vol. 12 (08): 619-621 [Abstract] ( 6278 ) [HTML 1KB] [PDF 955KB] ( 2060 )
622 TIAN Luan-Ying, Aaron HAMVAS
Risk factors for nosocomial bloodstream infections in a neonatal intensive care unit
OBJECTIVE: To assess the risk factors for nosocomial blood-stream infection (BSI) in a neonatal intensive care unit (NICU). METHODS: Clinical data from the neonates admitted to the NICU in the St. Louis Children’s Hospital in Washington University School of Medicine between January 2005 and December 2006 were retrospectively studied. RESULTS: A total of 1 290 neonates were included. Overall, 175 nosocomial BSIs occurred. Catheter-related BSIs accounted for 62.3% (109 cases). The incidence of nosocomial BSI was 4.22 per 1 000 patient-days. Logistic regression analysis revealed that low gestational age, low Apgar scores at 5 minutes, use of central venous catheter (CVC), and longer CVC use were risk factors for the development of nosocomial BSI. In the subgroup of neonates with CVC, mechanical ventilation was an additional independent risk factor for BSI. CONCLUSIONS: Catheter-related BSI is the major source of nosocomial BSI in the NICU. Prematurity, low Apgar scores at birth and prolonged CVC use are risk factors for the development of BSI.［Chin J Contemp Pediatr, 2010, 12 (8):622-624］
2010 Vol. 12 (08): 622-624 [Abstract] ( 5774 ) [HTML 1KB] [PDF 961KB] ( 2931 )
625 LIU Xue-Ru, NONG Guang-Min
Primary immunodeficiency diseases in children: clinical analysis of 35 cases
OBJECTIVE: To summarize clinical features of primary immunodeficiency diseases (PID) in children. METHODS: The clinical data of 35 children with PID from September 2005 to December 2008 were studied retrospectively, including illness history, birth history, family history, clinical manifestations, laboratory findings, diagnosis, treatment and outcome. RESULTS: Of the 35 cases of PID, 6 cases were confirmed with combined T- and B-cell immunodeficiency, 4 cases with X-linked agammaglobulinaemia, 22 cases with selective IgG subclass deficiency, 1 case with common variable immunodeficiency and 2 cases with chronic granulomatous disease. All cases had fever and recurrent infections. Respiratory and digestive tract infections were the most common clinical manifestation. Some of the PID cases lagged behind the normal children of the same age in growth and development. Human γ-globulin transfusion and anti-infection therapy were administered. Two patients discontinued the therapy, one was transferred to the other hospital and the other 32 patients were discharged following improvement in clinical symptoms. CONCLUSIONS: PID should be considered in children who suffer from recurrent infections and autoimmune diseases or do not respond to long-term use of antibiotics. Immunologic tests should be done as early as possible for the children.［Chin J Contemp Pediatr, 2010, 12 (8):625-629］
2010 Vol. 12 (08): 625-629 [Abstract] ( 6303 ) [HTML 1KB] [PDF 980KB] ( 2077 )
630 WEI Min-Jiang, WU Wei-Lan, CHEN Min-Yi
Expression of TGF-β and hepatocyte growth factor in kidney tissues of children with primary focal segmental glomerular sclerosis
OBJECTIVE: To study the expression of transforming growth factor-β (TGF-β) and hepatocyte growth factor (HGF) in kidney tissues of children with primary focal segmental glomerular sclerosis (FSGS) and the possible role of the two growth factors in the development of FSGS. METHODS: Kidney specimens were obtained from 33 children with primary FSGS and 7 children with isolated haematuria but without FSGS (control group). Of the 33 children with primary FSGS, 6 children had no renal tubule interstitial pathological damage (Experimental I group) and 27 children had renal tubule interstitial pathological damage (Experimental II group). Expression of TGF-β and HGF in kidney tissues was ascertained by the immunohistochemical method. RESULTS: TGF-β and HGF were expressed in the three groups, but there were significant differences among the three groups. The expression of TGF-β and HGF in the two experiment groups increased significantly compared with that in the control group. The Experimental II group had increased TGF-β expression but a significantly decreased HGF expression compared with the Experimental I group. The index of tubule interstitial pathological changes was positively correlated with the TGF-β expression (r=0.763, P<0.01), but negatively correlated with the HGF expression (r=-0.461, P<0.05) in the Experimental II group. There was a negative correlation between TGF-β and HGF expression in children with primary FSGS (r=-0.425, P<0.05). CONCLUSIONS: The expression of TGF-β and HGF in kidney tissues is increased in children with primary FSGS. TGF-β might be a fibrogenic factor and HGF might be an anti-fibrotic factor in the kidney in primary FSGS.［Chin J Contemp Pediatr, 2010, 12 (8):630-633］
2010 Vol. 12 (08): 630-633 [Abstract] ( 5503 ) [HTML 1KB] [PDF 1401KB] ( 1947 )
634 CHEN Gan-Nong, MA Yong, YANG Zhu-Lin
Expression of GRP78 and GRP94 in the liver tissues and their clinicopathological significance in children with hepatoblastoma
OBJECTIVE: To study the expression of glucose-regulated protin 78 (GRP78) and glucose-regulated protin 94 (GRP94) in the liver tissues from children with hepatoblastoma (HB) and to investigate the possible clinicopathological values of GRP78 and GRP94 in HB. METHODS: Liver tissue specimens from 15 children with HB and 10 specimens of normal liver tissues were obtained. EnVison immunohistochemistry was used to detect the expression of GRP78 and GRP94 in the conventional paraffin-embedded liver sections. RESULTS: The positive rates of GRP78 expression (53% vs 10%; P<0.05) and GRP94 expression (60% vs 10%; P<0.05) in HB liver tissues were significantly higher than those in the normal liver tissues. The positive rates of GRP78 expression in the cases without lymphnode metastasis or in clinical stage I-II were significantly lower than those in the cases with lymphnode metastasis or in clinical stage III-IV (P<0.05). GRP94 showed a decreased tendency of positive expression in the cases without lymphnode metastasis or in clinical stage I-II when compared with the cases with lymphnode metastasis or in clinical stage III-IV, although there were no statistical differences between them. CONCLUSIONS: GRP78 and GRP94 expression might play important roles in the pathogenesis and progression of pediatric HB.［Chin J Contemp Pediatr, 2010, 12 (8):634-636］
2010 Vol. 12 (08): 634-636 [Abstract] ( 5992 ) [HTML 1KB] [PDF 965KB] ( 2131 )
637 LI Jie, LIU Rui, LI Zhu-Yi, WU Da-Fang, MA Xi-Juan, MIAO Jian-Ting
Clinical manifestations and neuroimaging characteristics of children with moyamoya disease
OBJECTIVE: To study the clinical manifestations and neuroimaging characteristics of pediatric moyamoya disease. METHODS: The clinical data of 17 children with moyamoya disease were retrospectively studied. RESULTS: The onset age was between 3 and 14 years. The main clinical manifestations included motor weakness of extremities or hemiplegia, sensory disturbance and headache. Cranial CT or/and MRI examinations predominately showed cerebral infarct. Magnetic resonance angiography (MRA) and digital subtraction angiography (DSA) showed stenosis or occlusion at the terminus of the siphon portions of internal carotid arteries and proximal portions of anterior or middle cerebral arteries, and abnormal vascular networks at the base of brain. CONCLUSIONS: Cerebral ischemia is main clinical manifestations in children with moyamoya disease, presenting motor weakness of extremities or hemiplegia, sensory disturbance and headache. DSA is essential to the diagnosis of the disease.［Chin J Contemp Pediatr, 2010, 12 (8):637-640］
2010 Vol. 12 (08): 637-640 [Abstract] ( 6069 ) [HTML 1KB] [PDF 1176KB] ( 2081 )
641 SHI Li-Wei, ZHAO Qun, ZHANG Li-Jun, LI Lian-Yong, GAO Hong
Distribution and expression of TGF-β2 in the capsule of children with developmental dysplasia of the hip
OBJECTIVE: This study examined the distribution and expression of transforming growth factor-β2 (TGF-β2) in the hip capsule of children with developmental dysplasia (dislocation) of the hip (DDH) and non-DDH children in order to investigate the roles of TGF-β2 in hip joint laxity. METHODS: Eight children with DDH and eight age- and gender-matched non-DDH children (control group) were enrolled. The immunohistochemical technique (S-P method) was used to examine the distribution and content of TGF-β2 in the hip capsule. Semiquantitative RT-PCR method was used to detect mRNA expression of TGF-β2 in the hip capsule. The quantitative analysis of TGF-β2 was performed by professional image software. RESULTS: A high expression of TGF-β2 was observed in the synovial layer with fibroblast regularly arranged parallel to the joint surface. There was decreased expression of TGF-β2 in the fibrous layer of the capsule. The percentage of positive fibroblasts and the gray-scale density in the fibrous layer in the DDH group were significantly lower than those in the control group (P<0.01). TGF-β2 mRNA expression in the DDH group decreased compared with that in the control group (P<0.05). CONCLUSIONS: The decreased TGF-β2 in distribution, content and mRNA expression in the hip capsule might contribute to hip joint laxity in children with DDH.［Chin J Contemp Pediatr, 2010, 12 (8):641-644］
2010 Vol. 12 (08): 641-644 [Abstract] ( 5529 ) [HTML 1KB] [PDF 1027KB] ( 1764 )
CHILD HEALTH CARE
645 JI Geng-Zhong, DENG Fang-Ming, WU Xin-Yin, LI Qing-Xiang, SONG Jian-Hui, LI Wen-Hai, YIN Fei
An epidemiologic survey on blood lead levels in preschool children living in towns of Hunan Province
OBJECTIVE: To investigate blood lead levels (BLLs) and influencing factors of BLLs among preschool children living in towns of Hunan Province. METHODS: A total of 2 044 preschool children (1 108 boys and 936 girls) from towns of 12 regions in Hunan Province were enrolled by a cluster sampling between September 2008 and June 2009. The average age of the children was 4.4±1.1 years (range 2 to 6 years). BLLs were determined using the atomic absorption spectrographic method. The influencing factors of BLLs were investigated using a standard questionnaire and logistic regression analysis. RESULTS: The mean BLLs of the children were 81.9±34.5 μg/L. BLLs more than 100 μg/ L were noted in 482 children (23.58%). Of the 482 children, 472 (23.09%) showed BLLs of 100-199 μg/L and 10 (0.49%) showed BLLs ≥200 μg/L. There were significant differences in the prevalence of elevated BLLs (≥100 μg/L) among different age groups (P<0.01). The prevalence of elevated BLLs in boys (28.99%) was significantly higher than that in girls (21.98%) (P<0.01). There were significant differences in the prevalence of elevated BLLs in children from different regions (P<0.01). The logistic regression analysis showed that the male(OR=1.449, P<0.01), father’s occupational lead exposure (OR=1.314, P<0.01)and maternal frequent use of hair dyes (OR=1.678, P<0.05) were risk factor for elevated BLLs. CONCLUSIONS: The prevalence of elevated BLLs is higher in preschool children living in towns of Hunan Province and is associated with a child’s region and age. The male, father’s occupational lead exposure and maternal frequent use of hair dyes are risk factor for elevated BLLs. ［Chin J Contemp Pediatr, 2010, 12 (8):645-649］
2010 Vol. 12 (08): 645-649 [Abstract] ( 5651 ) [HTML 1KB] [PDF 1041KB] ( 2232 )
EXPERIMENTAL RESEARCH
647 CHEN Wei-Chao, LIU En-Mei, DENG Yu, HE Yun, CHEN Jie-Hua, LI Xin, LIU Wei
Impact of neonatal bacillus Calmette-Guerin vaccination on lung Th17 cells and IL-17 in murine asthma model
OBJECTIVE: To study the impact of neonatal bacillus Calmette-Guerin(BCG) vaccination on lung Th17 cells and IL-17 in murine asthma model. METHODS: Neonatal BALB/c mice were divided into three groups: control, OVA and BCG/OVA groups. BCG was administerd in the BCG/OVA group on postnatal day 2 or 3. Except the control group, the mice in the other two groups were sensitized and undergone OVA challenge. Inflammatory cell numbers and morphological identification of leucocytes in bronchoalveolar lavage fluid (BALF) were measured by light microscopy. Cytokine IFN-γ and IL-17 levels in BALF were measured using ELISA. The percentage of lung Th17 cells were assayed by flow cytometry. RESULTS: There was significantly larger number of total cells, lymphocytes, eosinophils and neutrophils in BALF in the OVA and BCG/OVA groups compared with the control group. The number or percentage of those cells in the BCG/OVA group was lower than that in the OVA group. The level of IL-17 in BALF was significantly higher in the OVA and the BCG/OVA groups compared with the control group, while the level of IFN-γ was lower. The OVA group had higher level of IL-17 than the BCG/OVA group. The mice in the OVA and the BCG/OVA groups had a higher percentage of Th17 cells in lungs compared with the control group, but there were no significant differences in the percentage of Th17 cells between the OVA and the BCG/OVA groups. CONCLUSIONS: Th17 cells and IL-17 play roles in the pathogenesis of asthma. BCG vaccination can reduce the level of IL-17 in BALF and the reduced IL-17 may be mainly from other IL-17-producing cells in the lungs, not Th17 cells. ［Chin J Contemp Pediatr, 2010, 12 (8):650-653］
2010 Vol. 12 (08): 647-650 [Abstract] ( 7044 ) [HTML 1KB] [PDF 1119KB] ( 2489 )
654 LI Shao-Bo, TONG Xia-Sheng, WANG Xin-Xin, JIN Xiao-Hong, YE Hui
Regulative mechanism of budesonide on endogenous hydrogen sulfide, cystathionine-γ-lyase and cystathionine-β-synthase system in asthmatic rats
OBJECTIVE: To investigate plasma hydrogen sulfide (H2S) levels and cystathionine-γ- lyase (CSE) and cystathionine-β-synthase (CBS) mRNA expression in the lung tissues in asthmatic rats and to explore the roles of endogenous H2S, CSE and CBS system in the pathogenesis of asthma. METHODS: Thirty male Sprague-Dawley rats (age 5 to 7 weeks) were randomly divided into three groups: control, asthma and budesonide treatment (n=10 each). The asthma model was established by ovalbumin (OVA) sensitization and challenge. The budesonide treatment group received inhaled budesonide before challenge. The contents of plasma H2S were measured by spectrophotometry. The levels of CSE and CBS mRNA in the lung tissues were examined by reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: The contents of plasma H2S in the asthma group (61±16 μmol/L) were significantly lower than those in the control group(84±15 μmol/L) (P<0.01). The contents of plasma H2S in the budesonide treatment group (71±14 μmol/L) were not statistically different from those in the control and asthma groups. CSE mRNA and CBE mRNA expression in the asthma group were significantly lower than those in the control group (P<0.01). The budesonide treatment group had a decreased CSE mRNA expression and CBE mRNA expression compared with the control group, but had significantly increased CSE and CBE mRNA expression compared with the asthma group (P<0.01). There was a significantly negative correlation between H2S contents in plasma and total inflammatory cells in bronchoalveolar lavage fluid (n=30, r=-0.549, P<0.01). CONCLUSIONS: Plasma H2S levels and CSE and CBS expression in the lung decrease in asthmatic rats, which possibly promotes inflammatory cell aggregation to the airway. Budesonide may alleviate airway inflammation in asthmatic rats possibly through the system of endogenous H2S, CSE and CBS.［Chin J Contemp Pediatr, 2010, 12 (8):654-657］
2010 Vol. 12 (08): 654-657 [Abstract] ( 6298 ) [HTML 1KB] [PDF 1209KB] ( 1755 )
658 HUA Bing, DONG Wen-Bin, LI Qing-Ping, FENG Zhi-Qiang, YU Hong, ZHAI Xue-Song, LEI Xiao-Ping
Effects of Ucf-101 on expression of Omi/HtrA2 in kidneys of postasphyxial neonatal rats
OBJECTIVE: To investigate the expression of serine protease Omi/HtrA2 in kidneys of postasphyxial neonatal rats, and to study the effects of Ucf-101 on apoptosis and the expression of Omi/HtrA2 in these rats. METHODS: Seventy-two neonatal Wistar rats of 7-10 days old were randomly divided into 3 groups: control, postasphyxial model, Ucf-101-treated postasphyxial．The postasphyxial model was established by normobaric asphyxia．Expression of Omi/HtrA2 was determined with streptavidin-peroxidase immunohistochemistry 2, 24 and 48 hrs after asphyxia. Terminal deoxynuleotidyl-mediated nick end labeling (TUNEL) was used to ascertain the apoptosis of renal cells. RESULTS: Compared with the control group, Omi／HtrA2 expression in renal cells began to increase 2 hrs after asphyxia and peaked at 24 hrs. The expression of Omi/HtrA2 in the Ucf-101-treated postasphyxial group was significantly lower than that in the postasphyxial model group (P<0.01). TUNEL-positive cells began to increase 2 hrs after asphyxia and peaked at 24 hrs in the postasphyxial model group when compared with the control group. The number of TUNEL-positive cells in the Ucf-101-treated postasphyxial group was significantly lower than that in the postasphyxial model group at all time points (P<0.01). CONCLUSIONS: The expression of Omi/HtrA2 in kidneys is increased in postasphyxial neonatal rats. The increased Omi/HtrA2 expression may play an important role in the development of postasphyxial renal injury. Treatment with Ucf-101 can reduce the expression of Omi/HtrA2 in kidneys of postasphyxial neonatal rats and thus reduce renal tububar epithelial cell apoptosis.［Chin J Contemp Pediatr, 2010, 12 (8):658-661］
2010 Vol. 12 (08): 658-661 [Abstract] ( 6845 ) [HTML 1KB] [PDF 1226KB] ( 1912 )
662 ZHOU Hao, LIU Ye, LIANG Jin-Ping, SHAO Jian-Ling
Effect of maternal isolation stress on epilepsy susceptibility in young rats
OBJECTIVE: To study the effect of maternal isolation stress on the epilepsy susceptibility in young rats and the possible mechanism. METHODS: Sixty Sprague-Dawley young rats were randomly divided into a normal control and two maternal isolation groups that were subjected to maternal isolation for 15 min or 3 hrs daily on postnatal days 2-17. On postnatal day 18, an amygdala kindling test was performed to induce seizures. The expression of GABAA receptor α1 in the hippocampus was determined by immunohistochemisty. RESULTS: The weights were reduced, the threshold of amygdala kindling and the stimulation number for full kindling decreased significantly, and seizures were more severe in the maternal isolation 3 hrs group compared with the normal control group. The expression of GABAA receptor α1 in the hippocampus CA1 area in the maternal isolation 3 hrs group decreased significantly compared with that in the normal groups. There were no significant differences in the aspects above mentioned between the maternal isolation 15 min and normal control groups. CONCLUSIONS: The stress of early daily maternal isolation for 3 hrs may affect adversely brain development and increase epilepsy susceptibility in young rats. The decreased expression of GABAA receptor α1 in the hippocampus may contribute to the potential mechanism.［Chin J Contemp Pediatr, 2010, 12 (8):662-665］
2010 Vol. 12 (08): 662-665 [Abstract] ( 5201 ) [HTML 1KB] [PDF 1099KB] ( 1666 )
CLINICAL EXPERIENCE
666 ZHU Sheng, YI Li-Chun, SU Guo-Bing
Effect of minimally invasive surgery for intracranial hemorrhage

No abstravt available
2010 Vol. 12 (08): 666-667 [Abstract] ( 4283 ) [HTML 1KB] [PDF 947KB] ( 1427 )
668 ZHANG Xun, YANG Hong, XIE Qing, ZHANG Liang, LI Bo, JIAO Zheng, SUN Yi-Chun, ZHOU De-Zhong
Spontaneous intracranial hemorrhage in children: clinical analysis of 60 cases

No abstract available
2010 Vol. 12 (08): 668-669 [Abstract] ( 4448 ) [HTML 1KB] [PDF 939KB] ( 1722 )
670 SHEN Chen-Fu, YUAN Xian-Rui, LIU Jing-Ping, YU Xiao-He
Surgical treatment for skull or intracranial langerhan cell histiocytosis in children: experience of 13 cases
No abstract available
2010 Vol. 12 (08): 670-672 [Abstract] ( 4514 ) [HTML 1KB] [PDF 1097KB] ( 1743 )
673 ZHANG Zhi-Bo
Operation procedures for esophageal atresia complicated by other di-gestive malformations
No abstract available
2010 Vol. 12 (08): 673-674 [Abstract] ( 4002 ) [HTML 1KB] [PDF 934KB] ( 1494 )
675 WANG Rui-Geng, DENG Shu-Zhen, YIN Wei
Diagnosis of coronary artery dilatation secondary to Kawasaki disease by coronary angiography: analysis of 14 cases
No abstract available
2010 Vol. 12 (08): 675-676 [Abstract] ( 4272 ) [HTML 1KB] [PDF 935KB] ( 1927 )
681 JIANG Yong-Jiang, CHEN Ji-Chang, LI Cui-Yu
Sternal bone marrow aspiration in neonates: experience of 28 cases
No abstract available
2010 Vol. 12 (08): 681-681 [Abstract] ( 4675 ) [HTML 1KB] [PDF 929KB] ( 2126 )
CASE REPORT
676 SHI Pei-Pei, ZHANG Jian-Jiang
Eosinophilic gastroenteritis in a boy
No abstract available
2010 Vol. 12 (08): 676-677 [Abstract] ( 4772 ) [HTML 1KB] [PDF 1196KB] ( 1528 )
REVIEW
678 CHEN Qian, HU Zheng
Research advance in human bocavirus
No abstract available
2010 Vol. 12 (08): 678-680 [Abstract] ( 5026 ) [HTML 1KB] [PDF 957KB] ( 2272 )
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