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2010 Vol.  12 No.  11
Published: 2010-11-15
EXPERT LECTURE
CLINICAL RESEARCH
EXPERIMENTAL RESEARCH
CLINICAL EXPERIENCE
CASE REPORT
REVIEW
CLINICAL RESEARCH
849 DAI Yang-Li, FU Jun-Fen, LIANG Li, DONG Guan-Ping
A 10-year review of childhood type 1 diabetes mellitus and the clinical value of interleukin-10 in diabetic ketoacidosis
OBJECTIVE: To review the incident status of childhood type 1 diabetes mellitus hospitalized in the Children′s Hospital of Zhejiang University School of Medicine from 1999 to 2009 and to explore the clinical value of IL-10 in diabetic ketoacidosis. METHODS: The clinical data of 263 children with type 1 diabetes mellitus hospitalized in the Children′s Hospital of Zhejiang University School of Medicine from January 1999 to February 2009 were retrospectively reviewed. Serum lipid levels were measured in 48 children with type 1 diabetes mellitus and in 24 healthy children. The diabetic children were classified into two subgroups, with or without ketoacidosis. Serum lipid and cytokines levels were compared. RESULTS: Childhood type 1 diabetes mellitus was common in females (56.3%). The peak incident age of the disease was between 6 and 11.9 years. Diabetic ketoacidosis was as the presenting symptom for the first visit in 86 cases (32.7%). The levels of serum lipid, blood glucose and HbA1c in diabetic children with ketoacidosis were significantly higher than those without ketoacidosis (P<0.05). Logistic analysis demonstrated that the increased levels of blood glucose, serum lipid and HbA1c were risk factors for diabetic ketoacidosis. The level of serum IL-10 in diabetic children with ketoacidosis was significantly higher than that in patients without ketoacidosis (P<0.01), while there were no differences in serum levels IL-2, IL4, IL-6, TNF-α and IFN-γ between them. Serum levels IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ in diabetic children were significantly higher than those in healthy children (P<0.01). CONCLUSIONS: Ketoacidosis is a common acute complication of type 1 diabetes mellitus. The disorders of glucose and lipid metabolism are the risk factors for ketoacidosis in diabetic children. IL-10 may be a sensitive index of diabetic ketoacidosis in children with type 1 diabetes mellitus.［Chin J Contemp Pediatr, 2010, 12 (11):849-854］
2010 Vol. 12 (11): 849-854 [Abstract] ( 5359 ) [HTML 1KB] [PDF 1007KB] ( 1519 )
855 LUO Ying, TANG Li-Jun, HUANG Wei-Min
Levels of cytokines in peripheral blood of premature infants with chronic lung disease
OBJECTIVE: This study examined the levels of interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor alpha (TNF-α) in peripheral blood of premature infants with chronic lung disease (CLD) in order to investigate the possible role of immunologic factors in CLD. METHODS: Twenty-six premature infants who had been admitted to the neonatal intensive care unit for 28 days were classified into CLD (n=14) and non-CLD (control, n=12) groups. IL-1β, IL-6, IL-8 and TNF-α concentrations in peripheral blood were measured by multiplex technique (Bio-plex). RESULTS: There were no significant differences in peripheral blood levels of IL-1β, IL-6, IL-8 and TNF-α between the two groups. CONCLUSIONS: There are no significant changes in peripheral blood levels of IL-1β, IL-6, IL-8 and TNF-α in premature infants with CLD on the non-acute phase. Immunologic factors might not play a key role in CLD on the phase.［Chin J Contemp Pediatr, 2010, 12 (11):855-857］
2010 Vol. 12 (11): 855-857 [Abstract] ( 4614 ) [HTML 1KB] [PDF 897KB] ( 1209 )
858 ZHANG Zhi-Qun, HUANG Xian-Mei, LU Hui
Ambroxol for the prevention of respiratory distress syndrome in preterm infants: a Meta analysis
OBJECTIVE: To evaluate the efficacy and safety of ambroxol in the prevention of respiratory distress syndrome (RDS) in preterm infants. METHODS: Electronic searches were performed in the Cochrane Library, PubMED, EMBASE, Chinese CBM, Chinese VIP Database, Chinese Wanfang Database and Chinese CNKI Database up to the year of 2009 for randomized controlled trials (RCT) on ambroxol for the prevention of RDS in preterm infants. The meeting articles related to the RCT were manually searched in Pediatrics and Pediatric Research. Meta analysis was performed for the results of homogeneous studies by the Cochrane Collaboration′s software RevMan 5.0.17. RESULTS: Six RCTs involving 823 preterm infants were included, and the quality assessment for the trials demonstrated 1 article as A class, 1 article as B class and 4 articles as C class. The Meta analysis showed that ambroxol administration significantly reduced the incidence of RDS (OR=0.24, 95%CI: 0.15 - 0.64, P＜0.01), bronchopulmonary dysplasis (BPD, OR=0.41, 95%CI: 0.23 - 0.75, P＜0.01), intraventricular hemorrhage (IVH, OR=0.39, 95%CI:0.24 - 0.64, P＜0.01), patent ductus arteriosus (PDA, OR=0.33, 95%CI: 0.17 - 0.67, P＜0.01) and pulmonary infection (OR=0.24, 95%CI:0.14 - 0.38, P＜0.01). No adverse events related to the ambroxol treatment were reported. CONCLUSIONS: The current evidence shows that early use of ambroxol can reduce the risk of RDS, BPD, IVH, PDA and pulmonary infection in preterm infants.［Chin J Contemp Pediatr, 2010, 12 (11):858-863］
2010 Vol. 12 (11): 858-863 [Abstract] ( 8064 ) [HTML 1KB] [PDF 1328KB] ( 3396 )
864 ZHANG Li-Juan, WANG Bin, LIU Guo-Sheng
Relationship between serum estradiol levels in the early postnatal period and the occurrence of hyaline membrane disease and bronchopulmonary dysplasia in neonates
OBJECTIVE: This study examined the changes of serum levels of estradiol during the early postnatal period in neonates in order to investigate the possible relationship between the serum estradiol levels and the occurrence of pulmonary hyaline membrane disease (HMD) and bronchopulmonary dysplasia (BPD). METHODS: Fifty-nine premature infants with the gestational age between 26 and 32 weeks and 61 full-term infants with the gestational ages between 37 and 42 weeks were enrolled. Serum levels of estradiol were measured on postnatal days 1, 3 and 7. RESULTS: Serum levels of estradiol decreased rapidly after birth in both premature and term infants and there were significant differences among different postnatal ages groups. However, there were no significant differences in the serum estradiol levels between the premature and term groups on postnatal days 1, 3 and 7. Serum estradiol levels measured in premature infants with HMD were not statistically different from those in premature infants without HMD on all time points. Serum estradiol levels in premature infants with BPD were higher than those in premature infants without BPD on postnatal day 3, but there were no noticeable differences on postnatal days 1 and 7. CONCLUSIONS: Serum estradiol levels decline rapidly within 7 days after birth in both premature and term infants. Serum estradiol levels in the early postnatal period are not associated with the occurrence of HMD and BPD, suggesting that serum estradiol in the early postnatal period can not be used as a marker for predicting the development of HMD and BPD.［Chin J Contemp Pediatr, 2010, 12 (11):864-866］
2010 Vol. 12 (11): 864-866 [Abstract] ( 5194 ) [HTML 1KB] [PDF 889KB] ( 1418 )
867 ZHANG Cong, CHENG Hui, LV Jian
Influencing factors of umbilical cord separation in full-term newborns
OBJECTIVE: To investigate the influencing factors for the time of umbilical cord separation in full-term newborns. METHODS: The time of umbilical cord separation was recorded in 337 full-term newborns. Single factor and multifactor unconditioned logistic regression were performed to investigate the influencing factors of umbilical cord separation. Fourteen possible factors associated with the time of umbilical cord separation, including sex, gestational age, body weight, position of umbilical cord ligature, length of umbilical cord stump, umbilical cord diameter, cleanness of umbilical cord paster, hand cleanness of medical staff and family members and umbilical infection, were involved. RESULTS: The single factor correlative analysis demonstrated that the position of umbilical cord ligature, length of umbilical cord stump, umbilical cord diameter, cleanness of umbilical cord paster, and umbilical infection were influencing factors for the time of umbilical cord separation (P<0.05). The multifactor unconditioned logistic regression analysis demonstrated four major influencing factors for umbilical cord separation: position of umbilical cord ligature, length of umbilical cord stump, cleanness of umbilical cord paster, and umbilical infection. CONCLUSIONS: The following factors contribute to early separation of umbilical cord: the proper position of umbilical cord ligature (<0.5 cm to umbilical ring), the umbilical cord stump of <0.5 cm, keeping the umbilical cord paster clean and the prevention of umbilical infection.［Chin J Contemp Pediatr, 2010, 12 (11):867-869］
2010 Vol. 12 (11): 867-869 [Abstract] ( 5878 ) [HTML 1KB] [PDF 884KB] ( 1690 )
870 FAN Jiang-Hua, ZHU Yi-Min, ZHANG Xin-Ping
Correlation of hypoproteinemia with C-reactive protein and procalcitonin in children with sepsis
OBJECTIVE: To investigate the correlation of hypoproteinemia with inflammation parameters C-reactive protein (CRP), procalcitonin (PCT) and WBC in children with sepsis. METHODS: Seventy-three children with sepsis (including 22 severe sepsis) and 40 non-sepsis children (control group) were enrolled. Serum albumin levels were measured on admission. Based on the level of serum albumin, 73 cases of sepsis were classified into three groups: mild hypoproteinemia, severe hypoproteinemia and normal albumin. Blood CRP, PCT and WBC levels were compared in the three groups. The correlation of CRP, PCT and WBC with serum albumin level was evaluated. RESULTS: Serum albumin levels in the sepsis groups (severe or non-severe) were significantly lower than those in the control group (P<0.05), and the severe sepsis group showed more decreased albumin levels compared with the non-severe sepsis group (P<0.05). Blood CRP, PCT and WBC levels in the mild hypoproteinemia group were higher than those in the normal albumin group (P<0.05), and the severe hypoproteinemia group showed more increased blood CRP, PCT and WBC levels compared with the mild hypoproteinemia group (P<0.05). The incidence of multiple organ failure in the severe hypoproteinemia group was significantly higher than that in the normal albumin group (P<0.05). Serum albumin levels were negatively correlated with blood CRP, PCT and WBC levels. CONCLUSIONS: Serum albumin levels decrease in children with sepsis, and the more serious the illness, the lower serum albumin levels, resulting in a worse prognosis. CRP, PCT and WBC are negatively correlated to serum albumin levels in children with sepsis.［Chin J Contemp Pediatr, 2010, 12 (11):870-873］
2010 Vol. 12 (11): 870-873 [Abstract] ( 6217 ) [HTML 1KB] [PDF 909KB] ( 1779 )
874 HAO Jin-Li, WANG Bao-Jin, Jean BAPTISTE
Efficacy of highly active antiretroviral therapy for childhood acquired immunodeficiency syndrome
OBJECTIVE: To investigate the efficacy of highly active antiretroviral therapy (HAART) for acquired immunodeficiency syndrome (AIDS) in children. METHODS: The clinical data of 38 children (2-15 years old) with AIDS from a region of Rwanda and who had received HAART were retrospectively reviewed. RESULTS: All of 13 children with anemia showed improved anemia symptoms after HAART. The hemoglobin contents returned to normal levels in 12 children with mild or moderate anemia. CD4 T lymphocytes increased by 24%-1 181% in 5 out of 6 cases with severe immunodeficiency after HAART. During the HAART, the weight gain averaged 2.3 kg yearly. The growth and development in 5 out of 8 children with delayed growth restored the levels of normal children of the same age after HAART. CONCLUSIONS: HAART can improve the health status in children with AIDS and is effective for childhood AIDS.［Chin J Contemp Pediatr, 2010, 12 (11):874-876］
2010 Vol. 12 (11): 874-876 [Abstract] ( 4733 ) [HTML 1KB] [PDF 883KB] ( 1093 )
EXPERIMENTAL RESEARCH
877 ZHENG Chang-Hua, TONG Xia-Sheng, LI Bin-Fei, ZHANG Wei-Xi, LI Chang-Chong
Expression of Rock2 and TGF-β1 mRNA and glucocorticoid intervention in asthmatic rats
OBJECTIVE: To study the roles of rhoassociated coiled coil forming protein kinase 2 (Rock2) and transforming growth factor-β1 (TGF-β1) mRNA in acute asthma and the effect of glucocorticoid intervention on the Rock2 and TGF-β1 mRNA expression in rats. METHODS: Forty-eight male rats were randomly divided into 4 groups (n=12 each): asthma, control, dexamethasone treated (DXM) and budesonide treated (BUD). Rat model of asthma was prepared by the ovalbumin (OVA) challenge. The animals were sacrificed 24 hrs after the last challenge. The total cell number and differentiation cell number were counted in bronchoalveolar lavage fluid (BALF). The protein expression of Rock2 was ascertained by immunohistochemistry and the mRNA expression of TGF-β1 was ascertained by hybridization in situ. RESULTS: The pathological changes in the BUD and the DXM groups were alleviated when compared with the asthma group. The total cell number and the percentage of eosinophil (EOS), polymorphonuclear leukocytes (PMN) and lymphocytes (Lym) in BALF in the asthma group were significantly higher than those in the control group (P<0.01). The percentage of macrophage (Mф) in the asthma group was significantly lower than that in the control group (P<0.01). The total cell number and the percentage of EOS and Lym in BALF in the DXM and the BUD groups decreased, while the percentage of Mф increased significantly compared with those in the asthma group (P<0.01). The Rock2 and TGF-β1 mRNA expression in lung tissues in the asthma group increased significantly compared with those in the control, BUD and DXM groups, while there were no significant differences in the Rock2 expression and TGF-β1 mRNA expression between the DXM or BUD group and the control group. CONCLUSIONS: The expression of Rock2 and TGF-β1 mRNA in lung tissues is increased in rats with acute asthma. Glucocorticoids can significantly decrease the expression of Rock2 and TGF-β1 in lung tissues, thus alleviates airway inflammation.［Chin J Contemp Pediatr, 2010, 12 (11):877-881］
2010 Vol. 12 (11): 877-881 [Abstract] ( 4839 ) [HTML 1KB] [PDF 1158KB] ( 1412 )
882 SUN Xiao-Jie, YU Li, WANG Xiao-Qin, LIU Zhong-Qiang, WANG Juan, GAO Zheng-Xiang, LIU Han-Min
Effects of endogenous carbon monoxide on gene expression profiles associated with the apoptosis of pulmonary arterial smooth muscle cells
OBJECTIVE: To identify the gene expression profiles associated with the apoptosis of pulmonary arterial smooth muscle cells stimulated by carbon monoxide (CO). METHODS: Primary cultured Sprague-Dawley rat pulmonary arterial smooth muscle cells (PASMC) were stimulated by platelet-derived growth factor (PDGF, 20 ng/mL) and hemin (20 μmol/L). Cells were harvested after 2 hrs and Affymetrix microarrays were used to detect the gene expression profile. RESULTS: Some genes associated with Map2k3 (P38) signal pathway, such as CyclinD1, CyclinH, CyclinL1, MAP2K3, Kras and Nras, were upregulated, but P27 expression was downregulated after PDGF treatment. After endogenous CO treatment, some genes associated with P53 pathway, such as Gadd45α, P21 and Trp53inp1, were upregulated. CONCLUSIONS: P53 pathway probably plays an important role in apoptosis of pulmonary arterial smooth muscle cells treated with endogenous CO.［Chin J Contemp Pediatr, 2010, 12 (11):882-885］
2010 Vol. 12 (11): 882-885 [Abstract] ( 4998 ) [HTML 1KB] [PDF 1016KB] ( 1345 )
886 LUO Qin, GAO Zheng-Xiang, CAO Li-Li, YU Li, WANG Tao, LIU Han-Min
Effect of spleen tyrosine kinase on the proliferation of pulmonary vascular smooth muscle cells in rats
OBJECTIVE: To investigate the role of spleen tyrosine kinase (Syk) in rat pulmonary vascular smooth muscle cells (PVSMCs) proliferation induced by platelet-derived growth factor-BB (PDGF-BB). METHODS: PVSMCs from male Sprague-Dawley rats were cultured in vitro and the cells of passages 3-5 were used in the experiment. PVSMCs were stimulated by PDGF-BB and were treated with three different doses of piceatannol, a Syk selective inhibitor. Cell proliferation was assessed by methyl thiazolyl tetrazolium (MTT) assay. DNA synthesis was measured by 3H-thymidine incorporation (3H-TdR). Cellular cycle was observed by flow cytometry. Syk mRNA and protein expression were detected using real-time quantitative PCR and Western blot, respectively. RESULTS: The expression of Syk protein of PVSMCs was significantly up-regulated following PDGF-BB stimulation. PDGF-BB stimulation dramatically increased PVSMCs proliferation. After piceatannol treatment, both Syk mRNA and protein expression decreased and the proliferation of PVSMCs was inhibited in a dose-dependent manner. CONCLUSIONS: Syk may promote PVSMCs proliferation induced by PDGF-BB.［Chin J Contemp Pediatr, 2010, 12 (11):886-890］
2010 Vol. 12 (11): 886-890 [Abstract] ( 5288 ) [HTML 1KB] [PDF 1108KB] ( 1459 )
891 CHEN Xiao-Qing, WU Sheng-Hua, ZHOU Xiao-Yu
Effects of antenatal administration of dexamethasone and betamethasone on signal transduction of bone morphogenetic protein in the fetal lungs of rats
OBJECTIVE: To study the role of antenatal glucocorticoid (dexamethasone and betamethasone) on bone morphogenetic protein (BMP) signal transduction of the rat fetal lungs. METHODS: Fifteen pregnant rats were randomly divided into five groups: the rats treated with dexamethasone for 1 day (1D-DEX) or 3 days (3D-DEX), with betamethasone for 1 day (1D-BEX) or 3 days (3D-BEX) or with normal saline (control group), followed cesarean section on the 19th day of gestation. The mRNA levels of BMP4, BMPR-II, Smad1 and ATF-2 of fetal rat lungs were ascertained by reverse transcriptase polymerase chain reaction (RT-PCR). The expression of BMP4, BMPR-II, Smad1 and ATF-2 antigen expression in fetal lungs was assessed by immune histochemical staining. The expression of BMP4 and BMPR-II was determined by Western blot. RESULTS: The levels of BMP4, BMPR-II and Smad1 mRNA expression were up-regulated in the 1D-BEX, 3D-BEX and 3D-DEX groups compared with those in the control group (P＜0.05). The immune histochemiscal analysis showed that the expression of BMP4, BMPR-II, Phospho-Smad1 (pSmad1) and ATF-2 in the 1D-BEX, 3D-BEX and 3D-DEX groups was significantly higher than that in the control group (P＜0.01). The results of Western blot demonstrated that the expression of BMP4 and BMPR-II protein increased significantly in the 1D-BEX, 3D-BEX and 3D-DEX groups when compared with the control group (P＜0.01). CONCLUSIONS: Betamethasone and dexamethasone may play important roles in the regulation of BMP signal transduction in the rat fetal lungs. Up-regulation of BMP4, BMPR-II and Smad1 might be one of crucial factors for the glucocorticoid-induced maturity of fetal lungs.［Chin J Contemp Pediatr, 2010, 12 (11):891-896］
2010 Vol. 12 (11): 891-896 [Abstract] ( 4713 ) [HTML 1KB] [PDF 1704KB] ( 1430 )
897 LI Zhong-Xia, JIA Xiu-Hong, LI Jian-Chang, HAN Lin
Effects of bone marrow stromal cells and VLA-4 antibody on apoptosis of childhood leukemia cells
OBJECTIVE: To study the protective effect of bone marrow stromal cells (BMSCs) upon childhood leukemia cells and the influence of VLA-4 antibody in vitro on leukemia cell apoptosis. METHODS: BMSCs from children with acute leukemia-were isolated by human lymphocyte separation medium. BMSCs (adherent) and leukemia cells (suspended) were cultured in vitro. This study included four groups: leukemia cells alone (control), leukemia cells+BMSCs, leukemia cells+BMSCs supernatant and leukemia cells+BMSCs+VLA-4 antibody. The apoptosis rate of leukemia cells in the four groups was determined by Annexin Ⅴ-FITC double-labeled flow cytometry. The expression of survivin and bcl-2 genes in leukemia cells was ascertained by RT-PCR. RESULTS: The apoptosis rate of leukemia cells in the leukemia cells+BMSCs and the leukemia cells+BMSCs supernatant groups was lower than that in the control group (P<0.05). Compared with the leukemia cells+BMSCs and the leukemia cells+BMSCs supernatant groups, the apoptosis rate of leukemia cells in the VLA-4 antibody group increased significantly (P<0.05). In the VLA-4 antibody group, the apoptosis rate of leukemia cells increased with prolonged culture time. There were significant differences in the apoptosis rate between 12 hrs and 24 hrs after VLA-4 antibody treatment (P<0.01). The expression of survivin and bcl-2 genes in leukemia cells from the VLA-4 antibody groups was reduced compared with that from the leukemia cells+BMSCs and the leukemia cells+BMSCs supernatant groups (P<0.05). CONCLUSIONS: BMSCs play protective roles on leukemia cells. VLA-4 antibody can block the adhesion between BMSCs and leukemia cells and promote leukemia cell apoptosis.［Chin J Contemp Pediatr, 2010, 12 (11):897-901］
2010 Vol. 12 (11): 897-901 [Abstract] ( 5107 ) [HTML 1KB] [PDF 1146KB] ( 1276 )
902 TONG Hai-Xia, LU Chun-Wei, WANG Li-Wei, WANG Qiu-Shi, ZHANG Ji-Hong
Role of caspase-8 in TRAIL-induced apoptosis of neuroblastoma cell lines
OBJECTIVE: The aim of this study was to investigate whether the induction of caspase-8 by γ-interferon (IFNγ) renders neuroblastoma (NB) cells sensitive to tumor necrosis factor related apoptosis inducing ligand(TRAIL). METHODS: Caspase-8 mRNA expression was determined by RT-PCR. The effects of IFNγ, TRAIL, IFNγ +TRAIL and caspase-8 inhibitor+ TRAIL on the growth and apoptosis of NB cells were detected with the methods of reduction rate of Alamar Blue assay and flow cytometry. The relative caspase-8 activity was measured with colorimetric assay. RESULTS: Caspase-8 expression was detectable in CHP212 cells which were sensitive to TRAIL, with an increased expression after treatment with IFNγ. Caspase-8 was undetectable in SH-SY5Y(SY5Y) cells which were resistant to TRAIL, but an increased expression of caspase-8 mRNA was found after treatment with IFNγ. Moreover, TRAIL combined with IFNγ induced apoptosis in SY5Y cells. The relative caspase-8 activity of CHP212 cells increased with the prolonged TRAIL action time. The relative caspase-8 activity of SY5Y cells in the IFNγ+TRAIL group was significantly higher than those of the control, IFNγ, TRAIL and inhibitor groups. CONCLUSIONS: NB cells expressing caspase-8 are sensitive to TRAIL. TRAIL induces apoptosis in NB cells with an increase of relative caspase-8 activity.［Chin J Contemp Pediatr, 2010, 12 (11):902-907］
2010 Vol. 12 (11): 902-907 [Abstract] ( 5091 ) [HTML 1KB] [PDF 1272KB] ( 1353 )
912 WANG Li-Min, CHI Ying-Jiao, WANG Li-Na, NIE Lei, ZOU Yan-Hong, ZHAO Ta-Na, LI Chun-Yu, CHEN Mei, HUO Ming-Xia
Expression of interleukin-6 in rat model of doxorubicin-induced nephropathy
OBJECTIVE: The pathogenesis of minimal change nephrotic syndrome (MCNS) remains unclear. This study aimed to investigate the expression of interleukin-6 (IL-6) in rats with doxorubicin-induced nephropathy and its possible roles in the pathogenesis of MCNS. METHODS: Eighty-three male Wistar rats were randomly assigned into a control group (n=32) and a nephropathy group (n=51). Nephropathy was induced by a single tail vein injection of doxorubicin (5 mg/kg). The control group was injected with normal saline. Twenty-four-hour urinary protein excretion was measured 7, 14, 28 and 42 days after doxorubicin injection. IL-6 expression in urine and renal tissues was determined using ELISA 7, 14, 28 and 42 days after doxorubicin injection. RESULTS: The urinary protein excretion increased significantly in the nephropathy group 7, 14, 28 and 42 days after doxorubicin injection compared with that in the control group (P<0.01). IL-6 expression in urine and renal tissues increased significantly 7, 14, 28 and 42 days after doxorubicin injection compared with that in the control group (P<0.01). IL-6 expression in urine and renal tissues was positively correlated with 24-hour urinary protein excretion in the nephropathy group (r=0.794, P<0.01; r= 0.870, P<0.01). IL-6 expression in urine was positively correlated with that in renal tissues (r=0.739, P<0.01). CONCLUSIONS: IL-6 expression in the urine and renal tissues is increased in MCNS rats. IL-6 might play an important role in the pathogenesis of MCNS.［Chin J Contemp Pediatr, 2010, 12 (11):912-914］
2010 Vol. 12 (11): 912-914 [Abstract] ( 4845 ) [HTML 1KB] [PDF 947KB] ( 1389 )
EXPERT LECTURE
908 CHEN Xiao-Cong, HUANG Shao-Ping, WANG Xue-Ying
Effect of astrocytes with different degrees of proliferation on multidrug resistance gene expression in rats with epilepsy
OBJECTIVE: To study the relationship of activated astrocytes and multidrug resistance gene (MDR) expression in rats with epilepsy. METHODS: Astrocytes of neonatal Sprague-Dawley rats were separated and cultured. The cultured cells of passage 3 were activated by TNF-α for 2, 24 or 48 hrs. The culture media of cells with different degrees of proliferation were infused to the lateral cerebral ventricle of rats with epilepsy. The expression of MDR in the brain tissue was ascertained by PCR, immunocytochemistry and Western blot. RESULTS: After 2 hrs of TNF-α stimulation, astrocytes began to proliferate, and reached a peak at 24 hrs. The expression of MDR in the brain tissue increased after infusion of culture medium of proliferated astrocytes in the TNF stimulation group compared with that in the control group without TNF stimulation. The level of MDR expression in the TNF stimulation group was positively correlated with the degrees of cell proliferation. CONCLUSIONS: Proliferation of astrocytes can increase the expression of MDR in rats with epilepsy and is probably involved in the development of refractory epilepsy.［Chin J Contemp Pediatr, 2010, 12 (11):908-911］
2010 Vol. 12 (11): 908-911 [Abstract] ( 5219 ) [HTML 1KB] [PDF 1183KB] ( 1343 )
CLINICAL EXPERIENCE
915 YOU Jie-Yu, ZHAO Hong-Mei, OU-YANG Wen-Xian, TANG Shuo, LIU Li, DUAN Bo-Ping
Anorectal motility in children with functional constipation
No abstract available
2010 Vol. 12 (11): 915-917 [Abstract] ( 4067 ) [HTML 1KB] [PDF 884KB] ( 1688 )
918 XIE Li-Juan, XIA Zheng-Rong, ZHANG Zhong-Yang, CHEN Guan-Yi, ZHU Jian-Xin
Application of susceptibility weighted imaging in brain injuries of preterm infants
No abstract available
2010 Vol. 12 (11): 918-921 [Abstract] ( 3639 ) [HTML 1KB] [PDF 1237KB] ( 1518 )
922 ZHANG Qian, CHENG Xin-Ru, SHENG Guang-Yao
Serum NT-proBNP levels in neonates with severe asphyxia and the effects of nalmefene on the NT-proBNP levels
No abstract available
2010 Vol. 12 (11): 922-923 [Abstract] ( 4742 ) [HTML 1KB] [PDF 875KB] ( 1459 )
CASE REPORT
924 XU Wei-Zhi, YANG Chun-Xiao
Prune Belly syndrome complicated by pectus excavatum, spleen and left kidney congenital absence and situs inversus viscerum: a case report
No abstract available
2010 Vol. 12 (11): 924-924 [Abstract] ( 3858 ) [HTML 1KB] [PDF 860KB] ( 1303 )
925 XU Yi-Ping, HUANG Ke
Infantile cortical hyperostosis: report of two cases
No abstract available
2010 Vol. 12 (11): 925-926 [Abstract] ( 3623 ) [HTML 1KB] [PDF 918KB] ( 1468 )
927 CHENG Liang, CHEN Chun-Yuan, GU Yong-Hong, WU Zhi-Xiang
Disseminated histoplasmosis in a boy presenting fever and jaundice as first manifestations
No abstract available
2010 Vol. 12 (11): 927-928 [Abstract] ( 3630 ) [HTML 1KB] [PDF 1085KB] ( 1151 )
929 CHEN Wei, ZHANG Qin, LIAO Jian-Xiang
Type 1 diabetes mellitus in both a boy and his younger sister
No abstract available
2010 Vol. 12 (11): 929-929 [Abstract] ( 3757 ) [HTML 1KB] [PDF 860KB] ( 1327 )
REVIEW
930 XU Xue-Feng, DU Li-Zhong
Role of reactive oxygen species in persistent pulmonary hypertension of the newborn
No abstract available
2010 Vol. 12 (11): 930-933 [Abstract] ( 3854 ) [HTML 1KB] [PDF 899KB] ( 1249 )
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