CJCP
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2011 Vol.  13 No.  3
Published: 2011-03-15
CLINICAL RESEARCH
EXPERIMENTAL RESEARCH
CLINICAL EXPERIENCE
CASE REPORT
CONFERENCE SUMMARY
REVIEW
CLINICAL RESEARCH
177 ZHANG Xiao-Rui, ZENG Chao-Mei, LIU Jie
Risk factors for preterm birth and complications in 287 late preterm infants
OBJECTIVE: To investigate the risk factors for preterm birth and complications in late preterm infants. METHODS: The clinical data of 287 late preterm infants were retrospectively studied. Two hundred and eighty-eight term infants served as the control group. Logistic regression analysis was used to identify risk factors associated with late preterm birth. The common complications in late preterm infants were investigated. RESULTS: Several significant risk factors for late preterm birth were identified by logistic regression analysis: twin pregnancy, gestational diabetes mellitus, eclampsia or preeclampsia, placenta previa, placental abruption and premature rupture of membranes. The duration of hospitalization in late preterm infants was longer than that in term infants. The complications were common in late preterm infants, with a high prevalence of anemia, aspiration pneumonia, hypoglycemia and intracranial hemorrhage. CONCLUSIONS: The late preterm infants are much more likely to suffer various complications. It is important to reduce the incidence of late preterm births by decreasing perinatal risk factors above mentioned.
2011 Vol. 13 (3): 177-180 [Abstract] ( 6586 ) [HTML 1KB] [PDF 1020KB] ( 2179 )
181 ZHOU Ying, WANG Le, LI Ming-Xia
Serum levels of HIF-1α, ET-1 and Ca2+ in neonates with hypoxic pulmonary hypertension
OBJECTIVE: To study the changes and significance of serum hypoxia-inducible factor-1α (HIF-1α), endothelin-1 (ET-1) and calcium (Ca2+) levels in neonates with hypoxic pulmonary hypertension (HPH). METHODS: Seventy-five neonates with HPH (29 mild, 25 moderate and 21 severe) and 22 hospitalized neonates with non-HPH (control group) were enrolled. Pulmonary artery systolic blood pressure (PASP) was measured by bedside echocardiography within 24 hrs after birth. Serum levels of HIF-1α and ET-1 were measured using ELASA. Serum Ca2+ concentrations were measured with ion selective electrode. RESULTS: Serum levels of HIF-1α and ET-1 in the HPH group increased significantly compared with those in the control group (P<0.01), and were positively related with PASP (Rhif-1α=0.75, P<0.01; Ret-1=0.56，P<0.05). Serum Ca2+ levels in neonates with severe HPH were significantly lower than those in the control group (P<0.05). There were no correlation between serum Ca2+ levels and PASP. CONCLUSIONS: Serum HIF-1α and ET-1 levels are positively related with PASP in neonates with HPH, suggesting that serum HIF-1α and ET-1 may be involved in the occurrence of neonatal HPH. Serum Ca2+ levels are reduced in severe neonates with HPH, suggesting that serum Ca2+ may play a role in the occurrence of severe HPH.
2011 Vol. 13 (3): 181-184 [Abstract] ( 5316 ) [HTML 1KB] [PDF 963KB] ( 1418 )
185 WU Liang-Xia, WU Min, GU Dan-Ping
Pulmonary functions in children with segmental Mycoplasma pneumoniae pneumonia
OBJECTIVE: To investigate the changes of pulmonary functions in children with segmental Mycoplasma pneumoniae pneumonia (SMPP). METHODS: A total of 55 children with SMPP were recruited into this study. Pulmonary functions were measured at both acute and recovery phases, including FVC, FEV1, FEV1/FVC, PEF, FEF25%, FEF50%, FEF75% and FEF25%-75%. RESULTS: FVC, FEV1, FEV1 /FVC, PEF, FEF25%, FEF50%, FEF75%, and FEF25%-75% were reduced in all of the 55 cases at the acute phase. FEF25%, FEF50%, FEF75% and FEF25%-75% decreased more significantly. The indexes above mentioned were improved significantly at the recovery phase compared with the acute phase (P<0.05). During the acute phase FVC and FEV1 decreased more significantly in the group with multiple area lesions than in the group with single area lesions (P<0.05). CONCLUSIONS: Both large and small airway functions are damaged in different degrees in children with SMPP during the acute phase. More cases show restrictive ventilatory disorders and the injury of small airway function is more severe. The pulmonary function is markedly improved at the recovery phase, suggesting that the pulmonary function impairments are reversible. The pulmonary function impairments are more severe in children with multiple area lesions.
2011 Vol. 13 (3): 185-187 [Abstract] ( 5626 ) [HTML 1KB] [PDF 930KB] ( 1484 )
188 KANG Yan-Meng, DING Ming-Jie, HAN Yu-Ling, WANG Shi-Fu, MA Xiang, LI Hua
Th1/Th2 immune response in bronchoalveolar lavage fluid in children with severe Mycoplasma pneumoniae pneumonia
OBJECTIVE: To study the status of Th1/Th2 immune response and the value of the detection of cytokines in bronchoalveolar lavage fluids (BALF) by examining the levels of IFN-γ and IL-4 in BALF and serum in children with severe Mycoplasma pneumonia (MPP). METHODS: The levels of IFN-γ and IL-4 in BALF and serum were measured using ELISA in 25 children with severe MPP, 25 children with mild MPP and 25 children with foreign body in bronchus. RESULTS: The levels of IL-4 and IFN-γ and the IL-4/IFN-γ ratio in BALF in children with severe MPP were significantly higher than those in children with foreign body in bronchus (P<0.01 or <0.05). The serum levels of IL-4 and the IL-4/IFN-γ ratio in children with severe MPP were significantly higher than those in children with foreign body in bronchus (P<0.01) or with mild MPP (P<0.05). The levels of IL-4 and the IL-4/IFN-γ ratio in BALF were significantly higher than in serum (P<0.05). CONCLUSIONS: The data suggest that the imbalance of Th1/Th2 exists in children with severe MPP and it seems to represent a predominant Th2-like cytokine response. The detection of cytokines in BALF appears to be more sensitive than in serum and may be of value in the diagnosis and therapy of MPP.
2011 Vol. 13 (3): 188-190 [Abstract] ( 6947 ) [HTML 1KB] [PDF 940KB] ( 2085 )
191 ZHONG Li-Li, PENG Li, HUANG Han, LIANG Mo, ZHOU Qiong-Hua, ZHANG Bing, LI Yun
Value of fluorescence quantitative PCR assay of bronchoalveolar lavage fluid samples in the diagnosis of Mycoplasma pneumoniae pneumonia in children
OBJECTIVE: To study the value of fluorescence quantitative polymerase chain reaction (FQ-PCR) for detecting Mycoplasma pneumoniae (MP)-DNA in bronchoalveolar lavage fluid (BALF) in the early diagnosis of Mycoplasma pneumoniae pneumonia (MPP). METHODS: FQ-PCR was used to detect MP-DNA in BALF obtained by fiberoptic bronchoscopy from 61 children with MPP, and the sensitivity and the specificity of FQ-PCR were compared with the traditional serological test. RESULTS: The sensitivity and the specificity of BALF FQ-PCR for detecting MP-DNA were 94% and 100% respectively. The accuracy of BALF FQ-PCR assay for detecting MP-DNA was higher than that of the serological test at the early stage of the disease (1-7 days) (P<0.01). In the children with refractory MPP, BALF FQ-PCR assay also showed higher accuracy for detecting MP-DNA than the serological test (P<0.01). The copies of MP-DNA in children with refractory MPP were significant higher than those in children with common MPP (P<0.05). The copies of MP-DNA were positively correlated with CRP values (r=0.845, P<0.01). CONCLUSIONS: FQ-PCR assay of BALF for detecting MP-DNA in BALF is superior to the serological test. It is a reliable method for the early diagnosis of MPP, especially refractory MPP. The copies of MP-DNA can be used as an index for evaluation of the treatment outcome of refractory MPP.
2011 Vol. 13 (3): 191-194 [Abstract] ( 6493 ) [HTML 1KB] [PDF 970KB] ( 1833 )
195 YAO Miao-Miao, WANG Ke-Ming, XU Qun-Ying, WANG Gui-Lan, LIU Xiang-Teng
Etiology and risk factors of infantile wheezing
OBJECTIVE: To study the etiology and risk factors of infantile wheezing. METHODS: The clinical data of 180 infants with wheezing were retrospectively studied. The risk factors for wheezing attacks were investigated by logistic regression analysis. RESULTS: Viral infection (33.3%) was the most common cause for wheezing attacks, followed by asthma (19.4%), parental smoking and special environments (15.6%), gastroesophageal reflux disease (12.8%), premature delivery (7.8%), Mycoplasma infection (6.7%), and bronchopulmonary dysplasia (4.4%). The multivariate logistic regression analysis showed 7 factors that significantly correlated with wheezing attacks: allergic history of parents, sensitization to alimentary or inspiratory allergens, viral or Mycoplasma infection, premature delivery and special environments. CONCLUSIONS: The commonest cause of infantile wheezing is viral infection, followed by asthma. Genetic factors, individual atopic constitution and environmental factors play important roles in wheezing attacks.
2011 Vol. 13 (3): 195-198 [Abstract] ( 6367 ) [HTML 1KB] [PDF 939KB] ( 1733 )
199 WANG Xiu-Fang, HOU Ying-Ying, BI Dan
Expression of serum Clara cell secretory protein 10 in wheezing children under 5 years old
OBJECTIVE: To study the expression of serum Clara cell secretory protein 10 (CC10) and total IgE concentration in wheezing children under 5 years old. METHODS: Fifty-nine children with recurrent wheezing under 5 years old were classified into two groups: wheezing group 1 with atopic high risks (n=33) and wheezing group 2 without atopic high risks (n=26). Twenty-three children without infectious diseases served as a control group. Serum levels of CC10 and IgE were measured using a solid-phase sandwich ELISA. RESULTS: The serum levels of CC10 in wheezing group 1 (3.95±1.26 ng/mL) and wheezing group 2 (5.41±1.64 ng/mL) were significantly lower than those in the control group (8.72±2.23 ng/mL; P<0.01). The wheezing group 1 showed more decreased serum levels of CC10 compared with wheezing group 2 (P<0.05). The serum IgE levels in wheezing group 1 were significantly higher than those in wheezing group 2 and the control group (P<0.05). There were no significant differences in serum IgE levels between the wheezing group 2 and control group. There was a negative correlation between serum levels of CC10 and IgE in wheezing group 1 (r=-0.912, P﹤0.01). CONCLUSIONS: Serum CC10 levels decrease remarkably in wheezing children, and more significant decrease is noted in patients with atopic high risks. Serum CC10 levels are negatively correlated to serum IgE levels in patients with atopic high risks.
2011 Vol. 13 (3): 199-201 [Abstract] ( 5338 ) [HTML 1KB] [PDF 955KB] ( 1071 )
202 LIU Yi-Hong, XIONG Li-Ping, SHEN Hong-Yuan
Changes of red cell immune function and T-lymphocyte subsets in children with bronchiolitis
OBJECTIVE: To study the changes of red cell immune function and T-lymphocyte subsets in children with bronchiolitis and their possible roles in the pathogenesis of bronchiolitis. METHODS: Forty-five children with bronchiolitis and 30 healthy controls were enrolled. Red cell immune complex rosette (RBC-ICR) and red cell C3b receptor rosette (RBC-C3bRR) were detected. The percentages of CD3+, CD4+ and CD8+ cells were assayed by flow cytometry. RESULTS: RBC-C3bRR［(13.6±6.2)% vs (18.0±7.4)%］and the percentage of CD8+ cells［(21.6±4.4)% vs (25.6±5.2) %］ in the bronchiolitis group were lower than those in the control group (P<0.01). The percentage of CD3+ cells ［(59.9±6.7)% vs (52.1±8.3)%］ and CD4+ cells［(53.5±6.2)% vs (46.8±4.9)%］and RBC-ICR［(8.3±3.5)% vs (6.1±2.5)%］in the bronchiolitis group were higher than those in the control group (P<0.01). The percentage of CD4+ cells was positively correlated with RBC-ICR (r=0.63,P<0.05) and negatively correlated with RBC-C3bRR (r=-0.82,P<0.01). CONCLUSIONS: There are dysfunctions of red cell immune and T-lymphocyte subsets in children with brochiolitis, which may play a role in the pathogenesis of brochiolitis.
2011 Vol. 13 (3): 202-204 [Abstract] ( 5510 ) [HTML 1KB] [PDF 929KB] ( 1229 )
205 LI Jin-Yi, JIA Yan-Jie, JIA Yong-Lin, LU Jing-Jing, JING Li-Jun, PENG Tao, LI Jing-Hong
Significance of soluble DLL1 in diagnosis of intracranial infectious diseases in children
OBJECTIVE: To investigate the significance of soluble DLL1 (Delta-like-1) levels of cerebrospinal fluid (CSF) and serum in the diagnosis of intracranial infection in children. METHODS: Fifty children with intracranial infection, including 20 cases of tuberculous meningitis (TM), 20 cases of viral meningitis (VM) and 10 cases of purulent meningitis (PM), and 20 children without intracranial infection (control group) were enrolled. The levels of soluble DLL1 in CSF and serum were measured using ELISA. RESULTS: The level of CSF soluble DLL1 in the TM group was significantly higher than that in the VM, PM and control groups (2.89±1.72 ng/mL vs 0.14±0.14 ng/mL, 0.27±0.21 ng/mL, 0.13±0.12 ng/mL; P<0.01). The level of serum soluble DLL1 in the TM group was also significantly higher than that in the VM, PM and control groups (12.61±6.45 ng/mL vs 2.28±2.27 ng/mL, 2.38±1.79 ng/mL, 2.26±2.10 ng/mL; P<0.01). The levels of soluble DLL1 in the CSF and serum in the VM and PM groups were not significantly different from those in the control group. CONCLUSIONS: Soluble DLL1 as a novel indicator might have potentially important value in the diagnosis of TM.
2011 Vol. 13 (3): 205-207 [Abstract] ( 4956 ) [HTML 1KB] [PDF 991KB] ( 1163 )
208 CHEN Jun, DUO Li-Kun
Changes of high-sensitivity CRP and insulin sensitivity index in children with obstructive sleep apnea hypopnea syndrome
OBJECTIVE: To investigate the levels of highsensitivity CRP (hsCRP) and insulin sensitivity index (ISI) in children with obstructive sleep apnea hypopnea syndrome (OSAHS). METHODS: Twenty-nine children with OSAHS and 22 children with primary snoring (PS) were enrolled. Polysomnography was performed. Body mass index (BMI), hsCRP, serum lipids, fasting plasma glucose (FPG), and insulin (INS) were measured. ISI was calculated. RESULTS: The apnea hypopnea index (AHI) in the OSAHS group was higher than that in the PS group (13.2±9.2 vs 1.2±1.1; P<0.05). The lowest oxygen saturation (LSaO2) in the OSAHS group was lower than that in the PS group ［(78.5±5.4)% vs (87.4±3.7)%; P<0.05］. The values of hsCRP in the OSAHS group was higher than those in the PS group (2.8±2.7 mg/L vs 0.6±0.9 mg/L; P<0.05). There were no significant differences in the ISI and serum lipids between the two groups. The hsCRP level was negatively correlated with LSaO2 in the OSAHS group (r=-0.531, P<0.05). CONCLUSIONS: The hsCRP level increases in children with OSAHS. The increased hsCRP level might be associated with an increased risk of cardiovascular diseases.
2011 Vol. 13 (3): 208-211 [Abstract] ( 5574 ) [HTML 1KB] [PDF 948KB] ( 1455 )
212 DENG Li-Sha, TENG Hui-Min, LI Yan-Shan
Clinical utility of reticulocyte hemoglobin content for the diagnosis of iron deficiency anemia in children
OBJECTIVE: To study the clinical utility of measuring reticulocyte hemoglobin content (CHr) in the diagnosis of iron deficiency anemia (IDA) in children. METHODS: One hundred children with IDA at ages of 1 to 6 years and 50 healthy children were enrolled. Red blood cell parameters, CHr, hemoglobin (Hb), red blood count (RBC) and mean corpusular volume (MCV), were determined using the Blood Cell Analyzer. Serum ferritin (SF) levels were determined using radioimmunoassay double antibody techique. Soluble serum transferrin (sTfR) levels were determined using ELISA. RESULTS: The values of Hb (100±6 g/L vs 126±8 g/L) and CHr (18±5 pg vs 31±3 pg) in the IDA group were significantly lower than normal controls (P<0.01). SF levels (11±4 μg/L) in the IDA group were also lower than normal controls (59±36 μg/L) (P<0.01). In contrast, the values of sTfR in the IDA group were significantly higher than normal controls (4.8±2.1 mg/L vs 1.4±0.6 mg/L; P<0.01). In both groups, there was a positive correlation between the values of CHr and Hb［r=0.540 (control group), r=0.734 (IDA group); P<0.01］. In the IDA group, CHr was positively correlated with SF(r=0.464; P<0.01) and negatively correlated with sTfR(r=-0.450; P<0.01). When the cut-off value of CHr was 27.8 pg, the sensitivity and specificity for the diagnosis of IDA were 88.0% and 90.0%, respectively and the area under the ROC curve was 0.948. CONCLUSIONS: CHr can be used as an index for the diagnosis of IDA in children.
2011 Vol. 13 (3): 212-215 [Abstract] ( 6292 ) [HTML 1KB] [PDF 1062KB] ( 1758 )
216 WEI Bin-Yuan, HUANG Fei, QIN Xiao-Tian, LIANG Qiao-Qi
Treatment of behavioral disorders by risperidone in children with autism
OBJECTIVE: To study the effect of risperidone treatment on behavioral disorders in children with autism. METHODS: Forty children with behavioral disorders (aged from 5 to 12 years) were treated with risperidone for 8 weeks. The behavioral symptoms were evaluated by the Clinical Global Impression (CGI) and the Autism Treatment Evaluation Checklist (ATEC) before and after the treatment. The adverse events related to risperidone treatment were observed. RESULTS: The score of severity of illness and the ATEC total scores were significantly reduced 8 weeks after risperidone treatment. Besides the social intercourse ability, great improvements have been shown on the verbal communication, apperception and behavioural symptoms by the ATEC. No severe adverse events related to risperidone treatment were observed. CONCLUSIONS: Risperidone can significantly improve the behavioral disorders in children with autism and is well-tolerated.
2011 Vol. 13 (3): 216-218 [Abstract] ( 6124 ) [HTML 1KB] [PDF 987KB] ( 1551 )
EXPERIMENTAL RESEARCH
219 LUAN Bin, FENG Xiao-Xia, YANG Yu-Xia, ZHANG Zhi-Ying
Roles of FIZZ1 and NOTCH1 in asthma
OBJECTIVE: To investigate the roles of FIZZ1 and NOTCH1 in the pathogenesis of asthma and the effect of rosiglitazone on airway remodeling. METHODS: Forty-five healthy 6 to 8-week-old Sprague-Dawley rats were randomly divided into a control group and asthma groups with and without rosiglitazone treatment. The paraffin slices of lung tissues were made to assess the histological changes. a-SMA protein, a specific marker of airway remodeling, in lung tissues was measured by immunohistochemistry. FIZZl-mRNA and NOTCH1-mRNA expression in lung tissues was measured by RT-PCR. RESULTS: The characteristic changes of airway remodeling were observed in the untreated asthma group. The histological changes in the airway were less severe in the rosiglitazone treated asthma group. Positive a-SMA staining, FIZZl-mRNA and NOTCH1-mRNA were highly expressed in peribronchial lung sections isolated from the untreated asthma group. Rosiglitazone treatment decreased significantly the expression of a-SMA protein, FIZZl-mRNA and NOTCH1-mRNA compared with the untreated asthma group, but the expression of a-SMA protein, FIZZl-mRNA and NOTCH1-mRNA in the rosiglitazone treated asthma group remained higher than the control group. a-SMA expression was positively correlated with FIZZl-mRNA (r=0.826, P＜0.01) and NOTCH1-mRNA expression (r=0.9, P<0.01). FIZZl-mRNA expression was positively correlated with NOTCH1-mRNA expression (r=0.76, P<0.01). CONCLUSIONS: FIZZl and NOTCH1 may induce an increase in a-SMA expression. FIZZl and NOTCH1 play a critical role in the process of airway remodeling. Rosiglitazone treatment may inhibit airway remodeling in asthmatic rats.
2011 Vol. 13 (3): 219-222 [Abstract] ( 5139 ) [HTML 1KB] [PDF 1352KB] ( 1321 )
223 ZOU Li-Ping, DU Wen-Li, ZHANG Yan, WANG Li-Xia
Expression of perforin and granzyme B in asthmatic rats and intervention of recombinant human growth hormone
OBJECTIVE: To study the expression of perforin and granzyme B (GzmB) in the lungs of asthmatic rats and the effect of recombinant human growth hormone (rhGH) on the expression. METHODS: Thirty Sprague-Dawley male rats were randomly divided into a normal control group and asthma groups with and without rhGH treatment. An asthma model was prepared by repeated sensitization with ovalbumin and aluminium hydroxide. The morphological changes of the airway were observed by hematoxylin and eosin staining. Terminal deoxyribonucleotide transferase-mediated Dutp-bintin (TUNLE) was used to detect the apoptosis of epithelial cells in the airway. RT-PCR was used to detect the mRNA transcripts of perforin and GzmB in the lung tissues. RESULTS: A significantly increased apoptosis rate of airway epithelial cells was noted in the untreated asthma group. The apoptosis rate was significantly ruduced in the rhGH-treated asthma group (P<0.05). Compared with the control group, perforin and GzmB expression in the lungs in the untreated asthma group increased significantly. The rhGH-treated asthma group demonstrated significantly decreased perforin (0.48±0.08 vs 0.63±0.08; P<0.05) and GzmB (0.44±0.13 vs 0.71±0.15; P<0.05) expression in the lungs compared with the untreated asthma group. Both PFP (r=0.800, P<0.05) and GzmB (r=0.806, P<0.01) were positively correlated with the apoptosis rate of airway epithelial cells. CONCLUSIONS: Perforin and GzmB may play important roles in the pathogenesis of asthma. rhGH treatment can inhibit apoptosis of airway epithelial cells and airway remodeling, possibly through a reduction in perforin and GzmB expression.
2011 Vol. 13 (3): 223-226 [Abstract] ( 5324 ) [HTML 1KB] [PDF 1297KB] ( 1140 )
227 GE Li-Sha, LI Chang-Chong, ZHANG Wei-Xi, LIANG Ya-Feng, ZHANG He
Effects of dexamethasone on 15-lipoxygenase expression in lungs of asthmatic rats
OBJECTIVE: 15-lipoxygenase (15-LO) is a prooxidant enzyme which is expressed in asthmatic lungs leading to formation of pro- and anti-inflammatory mediators. Gene expression profiling studies show the association between 15-LO and allergic asthma. This study was designed to observe the expression of 15-LO in lungs of asthmatic rats and examine the effects of dexamethasone on 15-lipoxygenase expression. METHODS: Twenty-seven male Sprague-Dawley (SD) rats were randomly divided into three groups: control, asthma and dexamethasone (DXM) intervention. An asthma model was prepared by sensitization and challenging with ovalbumin. The production of 15-LO in lung tissue homogenates was measured using ELISA.The expression of 15-LO mRNA in lungs was determined by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The levels of 15-LO mRNA and protein in the asthma group (0.51±0.14 and 2080±73 μg/mL, respectively) were lower than those in the control group (0.76±0.15 and 2472±106 μg/mL, respectively; P<0.01). DXM intervention increased significantly the levels of 15-LO mRNA and protein (1.02±0.34 and 2562±218 μg/mL) compared with the asthma group (P<0.01). CONCLUSIONS: The production of 15-LO in lung tissues is reduced in asthmatic rats. DXM can increase the expression of 15-LO in lung tissues and thus might provide anti-inflammatory effects in asthmatic rats.
2011 Vol. 13 (3): 227-230 [Abstract] ( 6268 ) [HTML 1KB] [PDF 1288KB] ( 1266 )
231 WANG Hai-Ping, DENG Xiao-Long, LI Guang-Qian
Effects of edravone on glial fibrillary acidic protein and interleukin-lβ expression and neuronal apoptosis in juvenile rat hippocampus after status convulsion
OBJECTIVE: To study the effects of edaravone on glial fibrillary acidic protein (GFAP) and interleukin-1β (IL-lβ) expression and neuronal apoptosis in the juvenile rat hippocampus after status convulsion (SC). METHODS: One hundred and ninety-five juvenile male Sprague-Dawley (SD) rats were randomly divided into 3 groups: normal saline control and SC with and without edaravone treatment. Each of the 3 groups was further subdivided into subgroups sacrificed at 4, 12, 24, 48 and 72 hrs after SC (n=15). The SC model was prepared using lithium-pilocarpine. The expression of GFAP and IL-lβ protein was detected with immunohistochemistry methods. The neuronal apoptosis was observed by TdT-mediated dUTP nick end labeling (TUNEL). The hippocampal GFAP mRNA expression was detected by RT-PCR. RESULTS: The value of IOD of GFAP and IL-lβ positive cells measured by immunohistochemistry in the untreated SC group increased compared with the control group. Expression of GFAP and IL-lβ protein was significantly reduced in the edaravone treated SC group compared with the untreated SC group. RT-PCR showed the expression trend of GFAP mRNA was similar to that of protein. The TUNEL positive cells in the hippocampus CA1 in the untreated SC group increased significantly 12 hrs after SC and reached a peak at 48 hrs compared with the control group. The intervention with edaravone decreased significantly TUNEL positive cells between 12-48 hrs after SC, but the number of TUNEL positive cells in the intervention group remained significantly greater than in the control group. CONCLUSIONS: The expression of GFAP and IL-lβ in the hippocampus increases after SC in rats. Edaravone may decrease the expression of GFAP and IL-1β and reduce the number of neuronal apoptosis. These results suggest that edaravone may have protective effects against brain damage caused by SC.
2011 Vol. 13 (3): 231-235 [Abstract] ( 5558 ) [HTML 1KB] [PDF 1575KB] ( 1286 )
236 FANG Qiong, CHEN Yan-Hui
Effects of nerve growth factor on neural cells in the epileptiform discharge region of the hippocampus in children with intractable epilepsy
OBJECTIVE: To study the effects of nerve growth factor (NGF) on the astrocytes and neurons in the epileptiform discharge region of the hippocampus in children with intractable epilepsy. METHODS: Acutely dissociated cells from the epileptiform discharge region of the hippocampus were exposed to blank control group and NGF treatment groups (NGF concentrations: 12.5, 50 and 100 ng/mL). Astrocytes and neurons were identified by bisbenzimide staining combined with the specific makers such as GFAP and MAP2. The total number of neural cells was counted under an inversion fluorescence microscope. The percentage of immunostaining positive cells was calculated. RESULTS: After 4 hrs of culture, the percentages of GFAP+ astrocytes and MAP2+ neurons in the NGF treatment groups were higher than those in the blank control group, and increased with increasing NGF concentration (P<0.05). CONCLUSIONS: NGF may have protective effects on GFAP+ astrocytes and MAP2+ neurons in the injured hippocampus of children with epilepsy.
2011 Vol. 13 (3): 236-239 [Abstract] ( 5122 ) [HTML 1KB] [PDF 1155KB] ( 1223 )
240 LI Kun-Xia, LI Ai-Min, ZHANG Ji-Hong
Effects of TrkB-BDNF signal pathway on synthesis and secretion of vascular endothelial growth factor in human neuroblastoma cells
OBJECTIVE: To study the effects of TrkB-BDNF signal pathway on the synthesis and secretion of vascular endothelial growth factor (VEGF) in human neuroblastoma cells (NB). METHODS: TrkB protein expression in SY5Y cells before and after all-trans-retinoicacid (ATRA) treatment was detected by Western blot. P-TrkB protein expression in SY5Y cells before and after the treatment of ATRA along with BDNF was also detected by Western blot. VEGF concentrations in the SY5Y cell culture supernatants were measured using ELISA after the treatment with ATRA, BDNF, tyrosine kinase inhibitor K252a and PI3k inhibitor LY294002. RESULTS: TrkB protein was undetectable in SY5Y cells before ATRA treatment. After the treatment of 1, 10 and 100 nM/L ATRA for five days, TrkB protein was expressed in SY5Y cells and the TrkB protein level increased with the increasing ATRA concentration. P-TrkB protein was not expressed in SY5Y cells treated only with 10 nM/L ATRA, but it was detectable after the treatment of ATRA along with BDNF. VEGF concentrations in the group treated with ATRA+BDNF were significantly higher than those in the untreated control and the ATRA alone treatment groups (P<0.01). VEGF concentrations in the K252a pretreated ATRA+BDNF group were significantly lower than those in the group treated with ATRA+BDNF (P＜0.05). VEGF concentrations in the LY294002 treatment group (ATRA+LY294002+BDNF group) were also significantly lower than those in the group treated with ATRA+BDNF (P＜0.01). CONCLUSIONS: Activation of TrkB-BDNF signal pathway may increase the synthesis and secretion of VEGF in human NB cells. The synthesis and secretion of VEGF can be inhibited by blocking TrkB-BDNF signal pathway with K252a or blocking the TrkB-BDNF downstream signal pathway PI3K/Akt with LY294002.
2011 Vol. 13 (3): 240-243 [Abstract] ( 7898 ) [HTML 1KB] [PDF 956KB] ( 1659 )
244 ZHANG Peng-Bo, LI Wei-Song, GAO Ming, LI Ling, WANG Ni, LEI Shan, LV Hai-Xia , CHEN Xin-Lin, LIU Yong
Culture and identification of neural stem cells from mouse embryos
OBJECTIVE: The purpose of this study was to culture and identify neural stem cells from mouse embryos in vitro using a modified method and provide a basis for further study of the biology of neural stem cells under hypoxia. METHODS: The cells were isolated mechanically from the front cortex of fetal Institute of Cancer Research (ICR) mice on embryonic day 14. They were passaged by mechanical dissociation and enzymatic digestion. The neurospheres were identified by immunofluorescent staining of nestin. Cell differentiation was induced by 1% fetal bovine serum and then the cells were identified by immunohistochemistry of β-tubulin III and GFAP. RESULTS: The cells obtained from the front cortex of fetal ICR mice had the capacity of forming neurospheres which showed nestin immunoreactive positivity. After being induced by 1% fetal bovine serum, the cells were differentiated into β-tubulin III-positive cells and GFAP-positive cells. CONCLUSIONS: Using mechanical dissociation of primary cells and mechanical dissociation with enzymatic digestion of primary cells, the NSCs from the front cortex of mouse embryos can be obtained.
2011 Vol. 13 (3): 244-247 [Abstract] ( 6225 ) [HTML 1KB] [PDF 1158KB] ( 1830 )
248 XU Ya-Li, CHEN Yi-Ji, ZHANG Ya-Wei
Retinoic acid protects neonatal rats from hyperoxia-induced lung injury through down-regulating connective tissue growth factor
OBJECTIVE: To explore the mechanism of retinoic acid (RA)　protection　against hyperoxia-induced lung injury. METHODS: Ninety Sprague-Dawley rats were randomly assigned into three groups (n=30 each): air control group (exposed to air) and hyperoxia groups (exposed to 85% oxygen) with and without RA treatment. The RA-treated hyperoxia group received an intraperitoneal injection of RA (500 μg/kg) daily. Lungs were removed by tnoracotomy 4, 7 and 14 days after exposure. Radical alveolar counts (RAC) were observed by hematoxylin and eosin staining under a light microscope. The mRNA level of CTGF in lungs was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The expression of CTGF protein in lungs was detected by immunohistochemistry. RESULTS: With the prolonged hyperoxia exposure, the lungs developed inflammatory cell infiltration, alveolar structure disorders, a decrease in the number of alveoli, and alveolar interstitial thickening in the hyperoxia groups with and without RA treatment. Pathological changes in the RA-treated hyperoxia group were less severe than the untreated hyperoxia group. The CTGF mRNA and protein expression were up-regulated in the hyperoxia groups with and without RA treatment 7 and 14 days after exposure compared with the air control group. Significantly decreased CTGF mRNA and protein expression were noted in the RA-treated hyperoxia group compared with the untreated hyperoxia group 14 days after exposure. CONCLUSIONS: The expression of CTGF mRNA and protein increases in neonatal rats with hyperoxia-induced lung injury. RA may provide protections against the lung injury possibly through down-regulating CTGF expression.
2011 Vol. 13 (3): 248-251 [Abstract] ( 5193 ) [HTML 1KB] [PDF 1200KB] ( 1083 )
CLINICAL EXPERIENCE
252 ZHOU Zhu-Liang, WANG Jian-Guo, PAN Tao, LI Chang-Chun, YANG Qi, ZHOU Min
Clinical and pathologic features of 33 children with thin basement membrane nephropathy
No abstract available
2011 Vol. 13 (3): 252-254 [Abstract] ( 3819 ) [HTML 1KB] [PDF 927KB] ( 1160 )
255 YANG Xue-Lei, HE Jian-Hua, YANG Xue-Tong
Detection of Rotavirus in 2745 children with diarrhea
No abstract avaibale
2011 Vol. 13 (3): 255-257 [Abstract] ( 3776 ) [HTML 1KB] [PDF 932KB] ( 1304 )
258 ZHAO Yuan, LI Juan, WEI Ke-Lun
Underlying diseases in neonates requiring mechanical ventilation
No abstract available
2011 Vol. 13 (3): 258-260 [Abstract] ( 3615 ) [HTML 1KB] [PDF 935KB] ( 1163 )
CASE REPORT
261 HE Li, XIONG Jie, HU Wan-Ru, ZHANG Qian-Zhong, HU Xiao-Bin, LI Ping
Acute lymphoblastic leukemia complicated by posterior reversible encephalopathy syndrome: a case report
No abstract available
2011 Vol. 13 (3): 261-262 [Abstract] ( 3696 ) [HTML 1KB] [PDF 970KB] ( 1103 )
263 LI Qiu-Ping, WANG Rui-Juan, FENG Zhi-Chun
Endogenous fungal endophthalmitis in a preterm infant
No abstract available
2011 Vol. 13 (3): 263-264 [Abstract] ( 4791 ) [HTML 1KB] [PDF 963KB] ( 1126 )
265 CHEN Mei, LU Li-Li, FENG Zhi-Chun
Recurrent ascites as a presenting manifestation of eosinophilic gastroenteritis: a case report
No abstract available
2011 Vol. 13 (3): 265-266 [Abstract] ( 4318 ) [HTML 1KB] [PDF 923KB] ( 1195 )
267 XIE Lei, SHENG Guang-Yao, WANG Ying-Chao
Systemic lupus erythematosus complicated by hypothyroidism: a case report
No abstract avaibale
2011 Vol. 13 (3): 267-267 [Abstract] ( 3910 ) [HTML 1KB] [PDF 901KB] ( 1267 )
REVIEW
268 LI Wen-Juan, CHEN Hui-Jin
Research progress in ischemia-induced abnormal glutamatergic signaling in immature cerebral white matter
No abstract available
2011 Vol. 13 (3): 268-272 [Abstract] ( 4307 ) [HTML 1KB] [PDF 949KB] ( 1136 )
CONFERENCE SUMMARY
273 MAO Jian, LIN Xin-Zhu
2010 USA Annual Meeting on Hot Topics in Neonatology
No abstract available
2011 Vol. 13 (3): 273-273 [Abstract] ( 3588 ) [HTML 1KB] [PDF 902KB] ( 1172 )
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